EC Number | Application | Comment | Organism |
---|---|---|---|
2.6.1.48 | synthesis | production of 5-aminovalerate and glutarate in Escherichia coli. Endogenous over-production of the precursor, lysine, is first achieved through metabolic deregulation of its biosynthesis pathway by introducing feedback resistant mutants of aspartate kinase III and dihydrodipicolinate synthase. Further disruption of native lysine decarboxylase activity limits cadaverine by-product formation. Co-expression of lysine monooxygenase and 5-aminovaleramide amidohydrolase then results in the production of 0.86 g/l 5-aminovalerate in 48 h. The additional co-expression of glutaric semialdehyde dehydrogenase and 5-aminovalerate aminotransferase leads to the production of 0.82 g/l glutarate under the same conditions. Yields on glucose are 71 and 68 mmol/mol for 5-aminovalerate and glutarate, respectively | Pseudomonas putida |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.1.20 | expressed in Escherichia coli BW25113(DE3) cells | Pseudomonas putida |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.6.1.48 | additional information | production of 5-aminovalerate and glutarate in Escherichia coli. Endogenous over-production of the precursor, lysine, is first achieved through metabolic deregulation of its biosynthesis pathway by introducing feedback resistant mutants of aspartate kinase III and dihydrodipicolinate synthase. Further disruption of native lysine decarboxylase activity limits cadaverine by-product formation. Co-expression of lysine monooxygenase and 5-aminovaleramide amidohydrolase then results in the production of 0.86 g/l 5-aminovalerate in 48 h. The additional co-expression of glutaric semialdehyde dehydrogenase and 5-aminovalerate aminotransferase leads to the production of 0.82 g/l glutarate under the same conditions. Yields on glucose are 71 and 68 mmol/mol for 5-aminovalerate and glutarate, respectively | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.20 | glutarate semialdehyde + NADP+ + H2O | Pseudomonas putida | - |
glutaric acid + NADPH + H+ | - |
? | |
1.2.1.20 | glutarate semialdehyde + NADP+ + H2O | Pseudomonas putida KT 2240 | - |
glutaric acid + NADPH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.20 | Pseudomonas putida | - |
- |
- |
1.2.1.20 | Pseudomonas putida KT 2240 | - |
- |
- |
2.6.1.48 | Pseudomonas putida | - |
- |
- |
2.6.1.48 | Pseudomonas putida KT 2240 | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.20 | glutarate semialdehyde + NADP+ + H2O | - |
Pseudomonas putida | glutaric acid + NADPH + H+ | - |
? | |
1.2.1.20 | glutarate semialdehyde + NADP+ + H2O | - |
Pseudomonas putida KT 2240 | glutaric acid + NADPH + H+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.20 | davDT davDT | - |
Pseudomonas putida |
1.2.1.20 | glutarate semialdehyde dehydrogenase | - |
Pseudomonas putida |