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Literature summary extracted from

  • Mahmud, A.; Hattori, K.; Hongwen, C.; Kitamoto, N.; Suzuki, T.; Nakamura, K.; Takamizawa, K.
    NAD+-dependent xylitol dehydrogenase (xdhA) and L-arabitol-4-dehydrogenase (ladA) deletion mutants of Aspergillus oryzae for improved xylitol production (2013), Biotechnol. Lett., 35, 769-777.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.9 gene xdhA Aspergillus oryzae
1.1.1.12 gene ladA Aspergillus oryzae

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.9 additional information generation of xdhA and ladA deletion mutants and double-deletion mutant, that show decreased dehydrogenase activities and increased xylitol production from D-xylose compared to the KBN616 wild-type strain, overview Aspergillus oryzae
1.1.1.9 additional information construction of deletion mutants of xdhA and ladA-xdhA, to generate mutants with decreased dehydrogenase activities and increased xylitol production. Genomic DNA from Aspergillus oryzae strain KBN616 is used as the template for amplification of the three xdhA and ladA inserts. Activities of xylitol dehydrogenase and xylose reductase in the mutant strains, phenotype, overview Aspergillus oryzae
1.1.1.12 additional information construction of deletion mutants of ladA and ladA-xdhA, to generate mutants with decreased dehydrogenase activities and increased xylitol production. Genomic DNA from Aspergillus oryzae strain KBN616 is used as the template for amplification of the three xdhA and ladA inserts. Activities of xylitol dehydrogenase and xylose reductase in the mutant strains, phenotype, overview Aspergillus oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.9 xylitol + NAD+ Aspergillus oryzae
-
 D-xylulose + NADH + H+
-
 r
1.1.1.9 xylitol + NAD+ Aspergillus oryzae
-
 D-xylulose + NADH + H+
-
 ?
1.1.1.9 xylitol + NAD+ Aspergillus oryzae KBN616
-
 D-xylulose + NADH + H+
-
 r
1.1.1.9 xylitol + NAD+ Aspergillus oryzae KBN616
-
 D-xylulose + NADH + H+
-
 ?
1.1.1.12 L-arabinitol + NAD+ Aspergillus oryzae
-
 L-xylulose + NADH + H+
-
 ?
1.1.1.12 L-arabinitol + NAD+ Aspergillus oryzae KBN616
-
 L-xylulose + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.9 Aspergillus oryzae
-
 gene xdhA
-
1.1.1.9 Aspergillus oryzae Q86ZV0 and uracil auxotroph derivative strain P4
-
1.1.1.9 Aspergillus oryzae KBN616
-
 gene xdhA
-
1.1.1.9 Aspergillus oryzae KBN616 Q86ZV0 and uracil auxotroph derivative strain P4
-
1.1.1.12 Aspergillus oryzae Q763T4 and uracil auxotroph derivative strain P4
-
1.1.1.12 Aspergillus oryzae KBN616 Q763T4 and uracil auxotroph derivative strain P4
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.9 xylitol + NAD+
-
 Aspergillus oryzae D-xylulose + NADH + H+
-
 r
1.1.1.9 xylitol + NAD+
-
 Aspergillus oryzae D-xylulose + NADH + H+
-
 ?
1.1.1.9 xylitol + NAD+
-
 Aspergillus oryzae KBN616 D-xylulose + NADH + H+
-
 r
1.1.1.9 xylitol + NAD+
-
 Aspergillus oryzae KBN616 D-xylulose + NADH + H+
-
 ?
1.1.1.12 L-arabinitol + NAD+
-
 Aspergillus oryzae L-xylulose + NADH + H+
-
 ?
1.1.1.12 L-arabinitol + NAD+
-
 Aspergillus oryzae KBN616 L-xylulose + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.9 D-xylulose reductase A
-
 Aspergillus oryzae
1.1.1.9 XDH
-
 Aspergillus oryzae
1.1.1.9 xdhA
-
 Aspergillus oryzae
1.1.1.9 xylitol dehydrogenase
-
 Aspergillus oryzae
1.1.1.12 L-arabitol dehydrogenase
-
 Aspergillus oryzae
1.1.1.12 LADA
-
 Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.9 25
-
 assay at Aspergillus oryzae
1.1.1.9 30
-
 assay at Aspergillus oryzae
1.1.1.12 25
-
 assay at Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.9 7
-
 assay at Aspergillus oryzae
1.1.1.9 7.5
-
 assay at Aspergillus oryzae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.9 NAD+
-
 Aspergillus oryzae
1.1.1.9 NADH
-
 Aspergillus oryzae
1.1.1.12 NAD+
-
 Aspergillus oryzae

General Information

EC Number General Information Comment Organism
1.1.1.9 malfunction deletin of gene xdhA and gene ladA and or both lead mutants with decreased dehydrogenase activities and increased xylitol production, overview Aspergillus oryzae
1.1.1.9 additional information xylitol production of wild-type and mutant strains, overview Aspergillus oryzae
1.1.1.12 additional information xylitol production of wild-type and mutant strains, overview Aspergillus oryzae