EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.11.40 | expression in Escherichia coli | Streptomyces hygroscopicus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.11.40 | Fe2+ | required for activity | Streptomyces hygroscopicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.11.40 | 38600 | - |
x * 38600, protein with an N-terminal His6 tag, calculated from sequence | Streptomyces hygroscopicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.11.40 | L-enduracididine + 2-oxoglutarate + O2 | Streptomyces hygroscopicus | biosynthesis of the nonproteinogenic amino acid beta-hydroxy-enduracididine, a component of the mannopeptimycins (cyclic glycopeptide antibiotics), produced by Streptomyces hygroscopicus NRRL 30439 | (3S)-3-hydroxy-L-enduracididine + succinate + CO2 | - |
? | |
1.14.11.40 | L-enduracididine + 2-oxoglutarate + O2 | Streptomyces hygroscopicus NRRL 30439 | biosynthesis of the nonproteinogenic amino acid beta-hydroxy-enduracididine, a component of the mannopeptimycins (cyclic glycopeptide antibiotics), produced by Streptomyces hygroscopicus NRRL 30439 | (3S)-3-hydroxy-L-enduracididine + succinate + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.11.40 | Streptomyces hygroscopicus | - |
- |
- |
1.14.11.40 | Streptomyces hygroscopicus NRRL 30439 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.11.40 | - |
Streptomyces hygroscopicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.11.40 | L-enduracididine + 2-oxoglutarate + O2 | biosynthesis of the nonproteinogenic amino acid beta-hydroxy-enduracididine, a component of the mannopeptimycins (cyclic glycopeptide antibiotics), produced by Streptomyces hygroscopicus NRRL 30439 | Streptomyces hygroscopicus | (3S)-3-hydroxy-L-enduracididine + succinate + CO2 | - |
? | |
1.14.11.40 | L-enduracididine + 2-oxoglutarate + O2 | MppO specifically hydroxylates L-enduracididine | Streptomyces hygroscopicus | (3S)-3-hydroxy-L-enduracididine + succinate + CO2 | - |
? | |
1.14.11.40 | L-enduracididine + 2-oxoglutarate + O2 | biosynthesis of the nonproteinogenic amino acid beta-hydroxy-enduracididine, a component of the mannopeptimycins (cyclic glycopeptide antibiotics), produced by Streptomyces hygroscopicus NRRL 30439 | Streptomyces hygroscopicus NRRL 30439 | (3S)-3-hydroxy-L-enduracididine + succinate + CO2 | - |
? | |
1.14.11.40 | L-enduracididine + 2-oxoglutarate + O2 | MppO specifically hydroxylates L-enduracididine | Streptomyces hygroscopicus NRRL 30439 | (3S)-3-hydroxy-L-enduracididine + succinate + CO2 | - |
? | |
1.14.11.40 | additional information | no activity with L-arginine or dideoxy-mannopeptimycin-gamma | Streptomyces hygroscopicus | ? | - |
? | |
1.14.11.40 | additional information | no activity with L-arginine or dideoxy-mannopeptimycin-gamma | Streptomyces hygroscopicus NRRL 30439 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.11.40 | ? | x * 38600, protein with an N-terminal His6 tag, calculated from sequence | Streptomyces hygroscopicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.11.40 | MppO | - |
Streptomyces hygroscopicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.11.40 | 30 | - |
assay at | Streptomyces hygroscopicus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.11.40 | malfunction | the role of MppO in beta-hydroxyenduracididine biosynthesis is confirmed through inactivation of mppO, which yields a strain that produces dideoxy-mannopeptimycins. MppO is responsible for introducing the beta-hydroxy group of both the D- and L-beta-hydroxyenduracididine residues | Streptomyces hygroscopicus |
1.14.11.40 | physiological function | biosynthesis of the nonproteinogenic amino acid beta-hydroxy-enduracididine, a component of the mannopeptimycins (cyclic glycopeptide antibiotics), produced by Streptomyces hygroscopicus NRRL 30439 | Streptomyces hygroscopicus |