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Literature summary extracted from

  • Stokke, R.; Karlstroem, M.; Yang, N.; Leiros, I.; Ladenstein, R.; Birkeland, N.K.; Steen, I.H.
    Thermal stability of isocitrate dehydrogenase from Archaeoglobus fulgidus studied by crystal structure analysis and engineering of chimers (2007), Extremophiles, 11, 481-493.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.42 hanging drop vapour diffusion technique with reservoir solution consisting of 0.6 M ZnSO4 and 0.1 M Na cacodylate, pH 6.3 Archaeoglobus fulgidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.42 additional information
-
 additional information Km-values of wild-type and chimeric enzymes Escherichia coli
1.1.1.42 additional information
-
 additional information Km-values of wild-type and chimeric enzymes Archaeoglobus fulgidus
1.1.1.42 0.0165
-
 NADP+ 60°C, pH 8.0 Archaeoglobus fulgidus
1.1.1.42 0.0392
-
 NADP+ 40°C, pH 8.0 Escherichia coli
1.1.1.42 0.0405
-
 isocitrate 40°C, pH 8.0 Escherichia coli
1.1.1.42 0.332
-
 isocitrate 60°C, pH 8.0 Archaeoglobus fulgidus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.42 Zn2+ a zinc ion is tightly bound to Asp301, Asp305, Asp277 in the active site of subunit A and subunit B Archaeoglobus fulgidus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.42 45800
-
-
 Escherichia coli
1.1.1.42 45800
-
-
 Archaeoglobus fulgidus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Archaeoglobus fulgidus O29610
-
-
1.1.1.42 Escherichia coli P08200
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.42
-
 Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.42 isocitrate + NADP+
-
 Escherichia coli 2-oxoglutarate + CO2 + NADPH + H+
-
 ?
1.1.1.42 isocitrate + NADP+
-
 Archaeoglobus fulgidus 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.42 AfIDH
-
 Archaeoglobus fulgidus
1.1.1.42 EcIDH
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.42 40
-
 assay at Escherichia coli
1.1.1.42 50
-
-
 Escherichia coli
1.1.1.42 60
-
 assay at Archaeoglobus fulgidus
1.1.1.42 90
-
 about Archaeoglobus fulgidus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.42 additional information
-
 thermal stability of wild-type and chimeric enzymes Escherichia coli
1.1.1.42 additional information
-
 thermal stability of wild-type and chimeric enzymes Archaeoglobus fulgidus
1.1.1.42 50
-
 t1/2: 24.2 min Escherichia coli
1.1.1.42 65
-
 t1/2: 167 min Archaeoglobus fulgidus
1.1.1.42 70
-
 t1/2: 106 min Archaeoglobus fulgidus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.42 additional information
-
 additional information turnover numbers of wild-type and chimeric enzymes Escherichia coli
1.1.1.42 additional information
-
 additional information turnover numbers of wild-type and chimeric enzymes Archaeoglobus fulgidus
1.1.1.42 88.1
-
 NADP+ 40°C, pH 8.0 Escherichia coli
1.1.1.42 106.4
-
 isocitrate 40°C, pH 8.0 Escherichia coli
1.1.1.42 219
-
 NADP+ 60°C, pH 8.0 Archaeoglobus fulgidus
1.1.1.42 255
-
 isocitrate 60°C, pH 8.0 Archaeoglobus fulgidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.42 8
-
 assay at Escherichia coli
1.1.1.42 8
-
 assay at Archaeoglobus fulgidus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.42 additional information
-
 additional information turnover numbers of wild-type and chimeric enzymes Escherichia coli
1.1.1.42 additional information
-
 additional information turnover numbers of wild-type and chimeric enzymes Archaeoglobus fulgidus
1.1.1.42 700
-
 isocitrate 60°C, pH 8.0 Archaeoglobus fulgidus
1.1.1.42 2200
-
 NADP+ 40°C, pH 8.0 Escherichia coli
1.1.1.42 2600
-
 isocitrate 40°C, pH 8.0 Escherichia coli
1.1.1.42 13300
-
 NADP+ 60°C, pH 8.0 Archaeoglobus fulgidus