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Literature summary extracted from

  • Esser, D.; Kouril, T.; Talfournier, F.; Polkowska, J.; Schrader, T.; Bräsen, C.; Siebers, B.
    Unraveling the function of paralogs of the aldehyde dehydrogenase super family from Sulfolobus solfataricus (2013), Extremophiles, 17, 205-216.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.16 expressed in Escherichia coli BL21 (DE3)-RIL cells Saccharolobus solfataricus
1.2.1.16 expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL cells Escherichia coli
1.2.1.24 expression in Escherichia coli Saccharolobus solfataricus
1.2.1.27 expression in Escherichia coli Saccharolobus solfataricus
1.2.1.27 SSO1218 expressed in Escherichia coli BL21 (DE3) RIL via isopropyl-b-D-thiogalactopyranoside induction Saccharolobus solfataricus
1.2.1.79 expression in Escherichia coli Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.24 2-oxoglutaric semialdehyde substrate inhibition Saccharolobus solfataricus
1.2.1.24 NAD+ substrate inhibition Saccharolobus solfataricus
1.2.1.24 succinate semialdehyde substrate inhibition Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.16 0.0011
-
 Succinic semialdehyde with NAD+ as cosubstrate, at pH 6.5 and 70°C Saccharolobus solfataricus
1.2.1.16 0.004
-
 Succinic semialdehyde with NADP+ as cosubstrate, at pH 6.5 and 70°C Saccharolobus solfataricus
1.2.1.16 0.01
-
 NADP+ at pH 6.5 and 70°C Saccharolobus solfataricus
1.2.1.16 0.03
-
 Succinic semialdehyde in 0.1 M HEPES/KOH (pH 6.5), at 70°C Escherichia coli
1.2.1.16 0.1
-
 2-oxoglutarate semialdehyde with NAD+ as cosubstrate, at pH 6.5 and 70°C Saccharolobus solfataricus
1.2.1.16 0.2
-
 2-oxoglutarate semialdehyde with NADP+ as cosubstrate, at pH 6.5 and 70°C Saccharolobus solfataricus
1.2.1.16 0.3
-
 2-oxoglutarate semialdehyde in 0.1 M HEPES/KOH (pH 6.5), at 70°C Escherichia coli
1.2.1.16 9.1
-
 NAD+ in 0.1 M HEPES/KOH (pH 6.5), at 70°C Escherichia coli
1.2.1.16 11.1
-
 NAD+ at pH 6.5 and 70°C Saccharolobus solfataricus
1.2.1.24 0.03
-
 succinate semialdehyde pH 6.5, 70°C, cofactor NAD+ Saccharolobus solfataricus
1.2.1.24 0.3
-
 2,5-dioxopentanoate pH 6.5, 70°C, cofactor NAD+ Saccharolobus solfataricus
1.2.1.24 9.1
-
 NAD+ pH 6.5, 70°C Saccharolobus solfataricus
1.2.1.79 0.001
-
 succinate semialdehyde pH 6.5, 70°C, cofactor NAD+ Saccharolobus solfataricus
1.2.1.79 0.004
-
 succinate semialdehyde pH 6.5, 70°C, cofactor NADP+ Saccharolobus solfataricus
1.2.1.79 0.01
-
 NADP+ pH 6.5, 70°C Saccharolobus solfataricus
1.2.1.79 0.1
-
 2,5-dioxopentanoate pH 6.5, 70°C, cofactor NAD+ Saccharolobus solfataricus
1.2.1.79 0.2
-
 2,5-dioxopentanoate pH 6.5, 70°C, cofactor NADP+ Saccharolobus solfataricus
1.2.1.79 11.1
-
 NAD+ pH 6.5, 70°C Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.16 51600
-
-
 Saccharolobus solfataricus
1.2.1.16 51600
-
 2 * 51600, SDS-PAGE Escherichia coli
1.2.1.16 114000
-
 gel filtration Escherichia coli
1.2.1.24 50000
-
 3 * 50000, SDS-PAGE Saccharolobus solfataricus
1.2.1.24 51500
-
 3 * 51500, calculated from sequence Saccharolobus solfataricus
1.2.1.24 157000
-
 gel filtration Saccharolobus solfataricus
1.2.1.27 50000
-
 SDS-PAGE Saccharolobus solfataricus
1.2.1.27 54600
-
 calculated from sequence Saccharolobus solfataricus
1.2.1.27 130000
-
 native molecular weight using gel filtration Saccharolobus solfataricus
1.2.1.79 50000
-
 2 * 50000, SDS-PAGE Saccharolobus solfataricus
1.2.1.79 51600
-
 2 * 51600, calculated from sequence Saccharolobus solfataricus
1.2.1.79 114000
-
 gel filtration Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.16 2-oxoglutarate semialdehyde + NAD+ + H2O Escherichia coli
-
 2-oxoglutarate + NADH + H+
-
 ?
1.2.1.16 2-oxoglutarate semialdehyde + NADP+ + H2O Escherichia coli
-
 2-oxoglutarate + NADPH + H+
-
 ?
1.2.1.16 acetaldehyde + NADP+ + H2O Escherichia coli
-
 succinate + NADPH + H+
-
 ?
1.2.1.16 additional information Escherichia coli almost no activity with DL-glyceraldehyde, DL-glyceraldehyde 3-phosphate, and glycoaldehyde ?
-
 ?
1.2.1.16 succinic semialdehyde + NAD+ + H2O Escherichia coli
-
 succinate + NADH + H+
-
 ?
1.2.1.27 2-methyl-3-oxopropanoate + CoA + H2O + NAD+ Saccharolobus solfataricus
-
 propanoyl-CoA + HCO3- + NADH
-
 ?
1.2.1.27 2-methyl-3-oxopropanoate + CoA + H2O + NAD+ Saccharolobus solfataricus P2
-
 propanoyl-CoA + HCO3- + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.16 Escherichia coli
-
-
-
1.2.1.16 Saccharolobus solfataricus
-
-
-
1.2.1.24 Saccharolobus solfataricus Q97XS9
-
-
1.2.1.27 Saccharolobus solfataricus Q97YT9
-
-
1.2.1.27 Saccharolobus solfataricus P2 Q97YT9
-
-
1.2.1.79 Saccharolobus solfataricus Q97XA5
-
-
1.2.1.79 Saccharolobus solfataricus P2 Q97XA5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.16 UNO Q-12 column chromatography Escherichia coli
1.2.1.16 UNO Q-12 column chromatography and Superdex 200 gel filtration Saccharolobus solfataricus
1.2.1.24
-
 Saccharolobus solfataricus
1.2.1.27
-
 Saccharolobus solfataricus
1.2.1.27 to apparent homogeneity using heat precipitation at 80°C for 20 min, ion exchange chromatography and gel filtration Saccharolobus solfataricus
1.2.1.79
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.16 2-oxoglutarate semialdehyde + NAD+ + H2O
-
 Escherichia coli 2-oxoglutarate + NADH + H+
-
 ?
1.2.1.16 2-oxoglutarate semialdehyde + NAD+ + H2O
-
 Saccharolobus solfataricus 2-oxoglutarate + NADH + H+
-
 ?
1.2.1.16 2-oxoglutarate semialdehyde + NADP+ + H2O
-
 Escherichia coli 2-oxoglutarate + NADPH + H+
-
 ?
1.2.1.16 2-oxoglutarate semialdehyde + NADP+ + H2O
-
 Saccharolobus solfataricus 2-oxoglutarate + NADPH + H+
-
 ?
1.2.1.16 acetaldehyde + NADP+ + H2O
-
 Escherichia coli succinate + NADPH + H+
-
 ?
1.2.1.16 additional information almost no activity with DL-glyceraldehyde, DL-glyceraldehyde 3-phosphate, and glycoaldehyde Escherichia coli ?
-
 ?
1.2.1.16 additional information almost no activity with D,L-glyceraldehyde, D,L-glyceraldehyde-3-phosphate, and D,L-glycolaldehyde Saccharolobus solfataricus ?
-
 ?
1.2.1.16 succinic semialdehyde + NAD+ + H2O
-
 Escherichia coli succinate + NADH + H+
-
 ?
1.2.1.16 succinic semialdehyde + NAD+ + H2O
-
 Saccharolobus solfataricus succinate + NADH + H+
-
 ?
1.2.1.16 succinic semialdehyde + NADP+ + H2O
-
 Saccharolobus solfataricus succinate + NADPH + H+
-
 ?
1.2.1.24 2,5-dioxopentanoate + NAD+ + H2O no activity in presence of NADP+ Saccharolobus solfataricus ?
-
 ?
1.2.1.24 additional information formaldehyde, betaine aldehyde and benzaldehyde can not serve as substrates Saccharolobus solfataricus ?
-
 ?
1.2.1.24 succinate semialdehyde + NAD+ + H2O no activity in presence of NADP+ Saccharolobus solfataricus succinate + NADH + 2 H+
-
 ?
1.2.1.27 2-methyl-3-oxopropanoate + CoA + H2O + NAD+
-
 Saccharolobus solfataricus propanoyl-CoA + HCO3- + NADH
-
 ?
1.2.1.27 2-methyl-3-oxopropanoate + CoA + H2O + NAD+
-
 Saccharolobus solfataricus P2 propanoyl-CoA + HCO3- + NADH
-
 ?
1.2.1.27 malonate semialdehyde + CoA + H2O + NAD+
-
 Saccharolobus solfataricus acetyl-CoA + CO2 + NADH
-
 ?
1.2.1.27 malonate semialdehyde + CoA + H2O + NAD+
-
 Saccharolobus solfataricus P2 acetyl-CoA + CO2 + NADH
-
 ?
1.2.1.27 malonate semialdehyde + CoA + NAD+
-
 Saccharolobus solfataricus acetyl-CoA + CO2 + NADH
-
 ?
1.2.1.27 malonate semialdehyde + CoA + NAD+
-
 Saccharolobus solfataricus P2 acetyl-CoA + CO2 + NADH
-
 ?
1.2.1.27 methylmalonate semialdehyde + CoA + H2O + NAD+
-
 Saccharolobus solfataricus propanoyl-CoA + HCO3- + NADH
-
 ?
1.2.1.27 additional information no activity is observed for the substrates D,L-glyceraldehyde, D,L-glyceraldehyde 3-phosphate and D,L-glycolaldehyde neither with NAD+ nor with NADP+ as cosubstrate Saccharolobus solfataricus ?
-
 ?
1.2.1.27 additional information no activity detected in the presence of NADP+ as cosubstrate, no activity with D,L-glyceraldehyde, D,L-glyceraldehyde-3-phosphate and D,L-glycolaldehyde - neither with NAD+ nor with NADP+ as cosubstrate Saccharolobus solfataricus ?
-
 ?
1.2.1.27 additional information no activity is observed for the substrates D,L-glyceraldehyde, D,L-glyceraldehyde 3-phosphate and D,L-glycolaldehyde neither with NAD+ nor with NADP+ as cosubstrate Saccharolobus solfataricus P2 ?
-
 ?
1.2.1.27 additional information no activity detected in the presence of NADP+ as cosubstrate, no activity with D,L-glyceraldehyde, D,L-glyceraldehyde-3-phosphate and D,L-glycolaldehyde - neither with NAD+ nor with NADP+ as cosubstrate Saccharolobus solfataricus P2 ?
-
 ?
1.2.1.27 propionaldehyde + CoA + H2O + NAD+ no physiological substrate Saccharolobus solfataricus ?
-
 ?
1.2.1.27 propionaldehyde + CoA + NAD+ highest activity, no physiological substrate for MSDH, it only forms the same thiopropionyl enzyme intermediate as methylmalonate semialdehyde and malonate semialdehyde Saccharolobus solfataricus ? + NADH
-
 ?
1.2.1.79 2,5-dioxopentanoate + NAD+ + H2O
-
 Saccharolobus solfataricus ?
-
 ?
1.2.1.79 2,5-dioxopentanoate + NAD+ + H2O
-
 Saccharolobus solfataricus P2 ?
-
 ?
1.2.1.79 2,5-dioxopentanoate + NADP+ + H2O
-
 Saccharolobus solfataricus ?
-
 ?
1.2.1.79 2,5-dioxopentanoate + NADP+ + H2O
-
 Saccharolobus solfataricus P2 ?
-
 ?
1.2.1.79 succinate semialdehyde + NAD+ + H2O kcat/Km for NADP+ is 250fold higher compared to kcat/Km for NAD+ Saccharolobus solfataricus succinate + NADH + 2 H+
-
 ?
1.2.1.79 succinate semialdehyde + NAD+ + H2O kcat/Km for NADP+ is 250fold higher compared to kcat/Km for NAD+ Saccharolobus solfataricus P2 succinate + NADH + 2 H+
-
 ?
1.2.1.79 succinate semialdehyde + NADP+ + H2O kcat/Km for NADP+ is 250fold higher compared to kcat/Km for NAD+ Saccharolobus solfataricus succinate + NADPH + 2 H+
-
 ?
1.2.1.79 succinate semialdehyde + NADP+ + H2O kcat/Km for NADP+ is 250fold higher compared to kcat/Km for NAD+ Saccharolobus solfataricus P2 succinate + NADPH + 2 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.16 ? x * 51600, SDS-PAGE Saccharolobus solfataricus
1.2.1.16 homodimer 2 * 51600, SDS-PAGE Escherichia coli
1.2.1.24 homotrimer 3 * 50000, SDS-PAGE Saccharolobus solfataricus
1.2.1.24 homotrimer 3 * 51500, calculated from sequence Saccharolobus solfataricus
1.2.1.27 homodimer 2 * 50000 Saccharolobus solfataricus
1.2.1.79 homodimer 2 * 50000, SDS-PAGE Saccharolobus solfataricus
1.2.1.79 homodimer 2 * 51600, calculated from sequence Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
1.2.1.16 ALDH
-
 Saccharolobus solfataricus
1.2.1.16 gapN-2
-
 Escherichia coli
1.2.1.16 gapN-2
-
 Saccharolobus solfataricus
1.2.1.16 SSADH-II
-
 Escherichia coli
1.2.1.16 SSADH-II
-
 Saccharolobus solfataricus
1.2.1.16 SSO1842
-
 Escherichia coli
1.2.1.16 SSO1842
-
 Saccharolobus solfataricus
1.2.1.16 succinic semialdehyde dehydrogenase
-
 Saccharolobus solfataricus
1.2.1.16 succinic semialdehyde:NAD(P)+ oxidoreductase
-
 Saccharolobus solfataricus
1.2.1.24 SSADH-I
-
 Saccharolobus solfataricus
1.2.1.24 SSO1629 ordered locus name Saccharolobus solfataricus
1.2.1.27 methylmalonate semialdehyde dehydrogenase
-
 Saccharolobus solfataricus
1.2.1.27 MSDH
-
 Saccharolobus solfataricus
1.2.1.27 SSO1218
-
 Saccharolobus solfataricus
1.2.1.79 SSADH-II
-
 Saccharolobus solfataricus
1.2.1.79 SSO1842 ordered locus name Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.24 70
-
 assay at Saccharolobus solfataricus
1.2.1.79 70
-
 assay at Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.16 7.1
-
 2-oxoglutarate semialdehyde in 0.1 M HEPES/KOH (pH 6.5), at 70°C Escherichia coli
1.2.1.16 16.5
-
 NADP+ at pH 6.5 and 70°C Saccharolobus solfataricus
1.2.1.16 16.8
-
 Succinic semialdehyde with NAD+ as cosubstrate, at pH 6.5 and 70°C Saccharolobus solfataricus
1.2.1.16 18.4
-
 Succinic semialdehyde with NADP+ as cosubstrate, at pH 6.5 and 70°C Saccharolobus solfataricus
1.2.1.16 21.2
-
 2-oxoglutarate semialdehyde with NADP+ as cosubstrate, at pH 6.5 and 70°C Saccharolobus solfataricus
1.2.1.16 22.6
-
 Succinic semialdehyde in 0.1 M HEPES/KOH (pH 6.5), at 70°C Escherichia coli
1.2.1.16 27.3
-
 2-oxoglutarate semialdehyde with NAD+ as cosubstrate, at pH 6.5 and 70°C Saccharolobus solfataricus
1.2.1.16 69
-
 NAD+ in 0.1 M HEPES/KOH (pH 6.5), at 70°C Escherichia coli
1.2.1.16 136.3
-
 NAD+ at pH 6.5 and 70°C Saccharolobus solfataricus
1.2.1.24 7.1
-
 2,5-dioxopentanoate pH 6.5, 70°C, cofactor NAD+ Saccharolobus solfataricus
1.2.1.24 22.6
-
 succinate semialdehyde pH 6.5, 70°C, cofactor NAD+ Saccharolobus solfataricus
1.2.1.24 69
-
 NAD+ pH 6.5, 70°C Saccharolobus solfataricus
1.2.1.27 0.003
-
 methylmalonate semialdehyde 30°C, pH 8.2 Saccharolobus solfataricus
1.2.1.27 0.003
-
 2-Methyl-3-oxopropanoate pH 8.2, 30°C Saccharolobus solfataricus
1.2.1.27 0.02
-
 malonate semialdehyde 30°C, pH 8.2 Saccharolobus solfataricus
1.2.1.27 0.02
-
 malonate semialdehyde pH 8.2, 30°C Saccharolobus solfataricus
1.2.1.27 0.03
-
 propionaldehyde 30°C, pH 8.2 Saccharolobus solfataricus
1.2.1.27 0.03
-
 propionaldehyde pH 8.2, 30°C Saccharolobus solfataricus
1.2.1.79 16.5
-
 NADP+ pH 6.5, 70°C Saccharolobus solfataricus
1.2.1.79 16.8
-
 succinate semialdehyde pH 6.5, 70°C, cofactor NAD+ Saccharolobus solfataricus
1.2.1.79 18.4
-
 succinate semialdehyde pH 6.5, 70°C, cofactor NADP+ Saccharolobus solfataricus
1.2.1.79 21.2
-
 2,5-dioxopentanoate pH 6.5, 70°C, cofactor NADP+ Saccharolobus solfataricus
1.2.1.79 27.3
-
 2,5-dioxopentanoate pH 6.5, 70°C, cofactor NAD+ Saccharolobus solfataricus
1.2.1.79 136.3
-
 NAD+ pH 6.5, 70°C Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.24 6.5
-
 assay at Saccharolobus solfataricus
1.2.1.79 6.5
-
 assay at Saccharolobus solfataricus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.16 NAD+
-
 Escherichia coli
1.2.1.16 NAD+
-
 Saccharolobus solfataricus
1.2.1.16 NADP+
-
 Saccharolobus solfataricus
1.2.1.16 NADP+ preferred cofactor Escherichia coli
1.2.1.24 NAD+ no activity in presence of NADP+ Saccharolobus solfataricus
1.2.1.27 CoA enzyme is strictly CoA-dependent Saccharolobus solfataricus
1.2.1.27 NAD+ no activity in the presence of NADP+ as cosubstrate Saccharolobus solfataricus
1.2.1.27 NAD+ cosubstrate Saccharolobus solfataricus
1.2.1.79 NAD+ kcat/Km for NADP+ is 250fold higher compared to kcat/Km for NAD+ Saccharolobus solfataricus
1.2.1.79 NADP+ kcat/Km for NADP+ is 250fold higher compared to kcat/Km for NAD+ Saccharolobus solfataricus

General Information

EC Number General Information Comment Organism
1.2.1.27 metabolism for MSDH, a major function in the degradation of branched chain amino acids is proposed which is supported by the high sequence homology with characterized MSDHs from bacteria Saccharolobus solfataricus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.1.16 12.3
-
 NAD+ in 0.1 M HEPES/KOH (pH 6.5), at 70°C Escherichia coli
1.2.1.16 12.3
-
 NAD+ at pH 6.5 and 70°C Saccharolobus solfataricus
1.2.1.16 134.1
-
 2-oxoglutarate semialdehyde in 0.1 M HEPES/KOH (pH 6.5), at 70°C Escherichia coli
1.2.1.16 134.1
-
 2-oxoglutarate semialdehyde with NADP+ as cosubstrate, at pH 6.5 and 70°C Saccharolobus solfataricus
1.2.1.16 215.3
-
 2-oxoglutarate semialdehyde with NAD+ as cosubstrate, at pH 6.5 and 70°C Saccharolobus solfataricus
1.2.1.16 1789
-
 Succinic semialdehyde with NAD+ as cosubstrate, at pH 6.5 and 70°C Saccharolobus solfataricus
1.2.1.16 3066
-
 NADP+ in 0.1 M HEPES/KOH (pH 6.5), at 70°C Escherichia coli
1.2.1.16 3454
-
 Succinic semialdehyde in 0.1 M HEPES/KOH (pH 6.5), at 70°C Escherichia coli
1.2.1.16 3454
-
 Succinic semialdehyde with NADP+ as cosubstrate, at pH 6.5 and 70°C Saccharolobus solfataricus
1.2.1.16 30660
-
 NADP+ at pH 6.5 and 70°C Saccharolobus solfataricus
1.2.1.24 7.6
-
 NAD+ pH 6.5, 70°C Saccharolobus solfataricus
1.2.1.24 21.4
-
 2,5-dioxopentanoate pH 6.5, 70°C, cofactor NAD+ Saccharolobus solfataricus
1.2.1.24 842
-
 succinate semialdehyde pH 6.5, 70°C, cofactor NAD+ Saccharolobus solfataricus
1.2.1.79 12.3
-
 NAD+ pH 6.5, 70°C Saccharolobus solfataricus
1.2.1.79 134.1
-
 2,5-dioxopentanoate pH 6.5, 70°C, cofactor NADP+ Saccharolobus solfataricus
1.2.1.79 215.3
-
 2,5-dioxopentanoate pH 6.5, 70°C, cofactor NAD+ Saccharolobus solfataricus
1.2.1.79 1789
-
 succinate semialdehyde pH 6.5, 70°C, cofactor NAD+ Saccharolobus solfataricus
1.2.1.79 3066
-
 NADP+ pH 6.5, 70°C Saccharolobus solfataricus
1.2.1.79 3454
-
 succinate semialdehyde pH 6.5, 70°C, cofactor NADP+ Saccharolobus solfataricus