EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.165 | expressed in Escherichia coli BL21(DE3)-RIL cells | Saccharolobus solfataricus |
2.7.1.165 | expression in Escherichia coli | Saccharolobus solfataricus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.165 | ATP | weak substrate inhibition | Saccharolobus solfataricus | |
2.7.1.165 | D-glycerate | substrate inhibition; substrate inhibition at glycerate concentrations higher than 2 mM at 80°C, above 2 mM at 70°C and 5 mM at 65°C | Saccharolobus solfataricus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.165 | Mg2+ | dependent on | Saccharolobus solfataricus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.165 | 44400 | - |
2 * 44400, calculated from amino acid sequence | Saccharolobus solfataricus |
2.7.1.165 | 44400 | - |
2 * 44400, calculated from sequence | Saccharolobus solfataricus |
2.7.1.165 | 49000 | - |
2 * 49000, SDS-PAGE | Saccharolobus solfataricus |
2.7.1.165 | 90000 | - |
gel filtration | Saccharolobus solfataricus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.165 | ATP + D-glycerate | Saccharolobus solfataricus | a key enzyme in the nonphosphorylative Entner-Doudoroff pathway in archaea. The glycerate kinase, with its unusual regulatory properties, seems to play a major role in controlling the flux between the glycolytic nonphosphorylative Entner-Doudoroff and the glycolytic/gluconeogenetic semiphosphorylative Entner-Doudoroff pathway | ADP + 2-phospho-D-glycerate | - |
? | |
2.7.1.165 | ATP + D-glycerate | Saccharolobus solfataricus P2 | a key enzyme in the nonphosphorylative Entner-Doudoroff pathway in archaea. The glycerate kinase, with its unusual regulatory properties, seems to play a major role in controlling the flux between the glycolytic nonphosphorylative Entner-Doudoroff and the glycolytic/gluconeogenetic semiphosphorylative Entner-Doudoroff pathway | ADP + 2-phospho-D-glycerate | - |
? | |
2.7.1.178 | ATP + 2-dehydro-3-deoxy-D-gluconate | Saccharolobus solfataricus | a key enzyme in the semiphosphorylative Entner-Doudoroff pathway in archaea | ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? | |
2.7.1.178 | ATP + 2-dehydro-3-deoxy-D-gluconate | Saccharolobus solfataricus | - |
ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate | - |
? | |
2.7.1.178 | ATP + 2-dehydro-3-deoxy-D-gluconate | Saccharolobus solfataricus P2 | a key enzyme in the semiphosphorylative Entner-Doudoroff pathway in archaea | ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? | |
2.7.1.178 | ATP + 2-dehydro-3-deoxy-D-gluconate | Saccharolobus solfataricus P2 | - |
ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.165 | Saccharolobus solfataricus | Q7LXP1 | - |
- |
2.7.1.165 | Saccharolobus solfataricus P2 | Q7LXP1 | - |
- |
2.7.1.178 | Saccharolobus solfataricus | - |
- |
- |
2.7.1.178 | Saccharolobus solfataricus | Q97U29 | - |
- |
2.7.1.178 | Saccharolobus solfataricus P2 | - |
- |
- |
2.7.1.178 | Saccharolobus solfataricus P2 | Q97U29 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.165 | - |
Saccharolobus solfataricus |
2.7.1.165 | UNO Q-12 column chromatography and Superdex 200 gel filtration | Saccharolobus solfataricus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.165 | ATP + D-glycerate | specific substrate | Saccharolobus solfataricus | ADP + 2-phospho-D-glycerate | - |
? | |
2.7.1.165 | ATP + D-glycerate | a key enzyme in the nonphosphorylative Entner-Doudoroff pathway in archaea. The glycerate kinase, with its unusual regulatory properties, seems to play a major role in controlling the flux between the glycolytic nonphosphorylative Entner-Doudoroff and the glycolytic/gluconeogenetic semiphosphorylative Entner-Doudoroff pathway | Saccharolobus solfataricus | ADP + 2-phospho-D-glycerate | - |
? | |
2.7.1.165 | ATP + D-glycerate | the enzyme is specific for D-glycerate. The enzyme shows cooperativity by D-glycerate and ATP. A good fit achieved from the kinetic model to the experimental data suggests a mechanism where two glycerate molecules bind to glycerate kinase and are converted to product, while a third binding site appears to be inhibitory. The model suggests that the inhibition appears to be due to the formation of an additional complex with very low activity at 80°C | Saccharolobus solfataricus | ADP + 2-phospho-D-glycerate | - |
? | |
2.7.1.165 | ATP + D-glycerate | specific substrate | Saccharolobus solfataricus P2 | ADP + 2-phospho-D-glycerate | - |
? | |
2.7.1.165 | ATP + D-glycerate | a key enzyme in the nonphosphorylative Entner-Doudoroff pathway in archaea. The glycerate kinase, with its unusual regulatory properties, seems to play a major role in controlling the flux between the glycolytic nonphosphorylative Entner-Doudoroff and the glycolytic/gluconeogenetic semiphosphorylative Entner-Doudoroff pathway | Saccharolobus solfataricus P2 | ADP + 2-phospho-D-glycerate | - |
? | |
2.7.1.165 | ATP + D-glycerate | the enzyme is specific for D-glycerate. The enzyme shows cooperativity by D-glycerate and ATP. A good fit achieved from the kinetic model to the experimental data suggests a mechanism where two glycerate molecules bind to glycerate kinase and are converted to product, while a third binding site appears to be inhibitory. The model suggests that the inhibition appears to be due to the formation of an additional complex with very low activity at 80°C | Saccharolobus solfataricus P2 | ADP + 2-phospho-D-glycerate | - |
? | |
2.7.1.178 | ATP + 2-dehydro-3-deoxy-D-gluconate | - |
Saccharolobus solfataricus | ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? | |
2.7.1.178 | ATP + 2-dehydro-3-deoxy-D-gluconate | a key enzyme in the semiphosphorylative Entner-Doudoroff pathway in archaea | Saccharolobus solfataricus | ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? | |
2.7.1.178 | ATP + 2-dehydro-3-deoxy-D-gluconate | - |
Saccharolobus solfataricus P2 | ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? | |
2.7.1.178 | ATP + 2-dehydro-3-deoxy-D-gluconate | a key enzyme in the semiphosphorylative Entner-Doudoroff pathway in archaea | Saccharolobus solfataricus P2 | ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? | |
2.7.1.178 | ATP + 2-dehydro-3-deoxy-D-gluconate | - |
Saccharolobus solfataricus | ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate | - |
? | |
2.7.1.178 | ATP + 2-dehydro-3-deoxy-D-gluconate | - |
Saccharolobus solfataricus P2 | ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.165 | homodimer | 2 * 49000, SDS-PAGE | Saccharolobus solfataricus |
2.7.1.165 | homodimer | 2 * 44400, calculated from amino acid sequence | Saccharolobus solfataricus |
2.7.1.165 | homodimer | 2 * 44400, calculated from sequence | Saccharolobus solfataricus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.165 | class II glycerate kinase | - |
Saccharolobus solfataricus |
2.7.1.165 | glycerate kinase | - |
Saccharolobus solfataricus |
2.7.1.165 | Sso-GK | - |
Saccharolobus solfataricus |
2.7.1.165 | SSO0666 | - |
Saccharolobus solfataricus |
2.7.1.178 | 2-keto-3-deoxygluconate kinase | - |
Saccharolobus solfataricus |
2.7.1.178 | KDGK | - |
Saccharolobus solfataricus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.165 | 80 | - |
- |
Saccharolobus solfataricus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.165 | 24.5 | - |
D-glycerate | in 100 mM HEPES/KOH, pH 7.0, at 37°C | Saccharolobus solfataricus | |
2.7.1.165 | 24.5 | - |
D-glycerate | pH 6.5, 80°C, Hill equation | Saccharolobus solfataricus | |
2.7.1.165 | 28.8 | - |
ATP | in 100 mM HEPES/KOH, pH 7.0, at 37°C | Saccharolobus solfataricus | |
2.7.1.165 | 28.8 | - |
ATP | pH 6.5, 80°C, MichaelisMenten equation | Saccharolobus solfataricus | |
2.7.1.165 | 35.1 | - |
ATP | pH 6.5, 80°C, substrate-inhibition model | Saccharolobus solfataricus | |
2.7.1.165 | 533.3 | - |
D-glycerate | pH 6.5, 80°C, kinetic model | Saccharolobus solfataricus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.165 | 0.1 | - |
D-glycerate | in 100 mM HEPES/KOH, pH 7.0, at 37°C | Saccharolobus solfataricus | |
2.7.1.165 | 0.1 | - |
D-glycerate | pH 6.5, 80°C | Saccharolobus solfataricus | |
2.7.1.165 | 61 | - |
ATP | in 100 mM HEPES/KOH, pH 7.0, at 37°C | Saccharolobus solfataricus | |
2.7.1.165 | 61 | - |
ATP | pH 6.5, 80°C | Saccharolobus solfataricus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.1.45 | malfunction | deletion of the semiphosphorylative Entner-Doudoroff pathway key enzyme 2-keto-3-deoxygluconate kinase in Sulfolobus solfataricus PBL2025 results in a similar growth phenotype on glucose as substrate compared with the wild-type. In contrast, the mutant shows strongly increased concentrations of nonphosphorylative-Entner-Doudoroff intermediates whereas the hexose and pentose phosphates as well as trehalose are decreased. The results indicate (a) that the nonphosphorylative pathway is able to compensate for the missing semiphosphorylative Entner-Doudoroff branch in glucose catabolism, (b) that in addition to its catabolic function the semiphosphorylative Entner-Doudoroff pathway has an additional although not essential role in providing sugar phosphates for anabolism/gluconeogenesis and (c) that glycerate kinase, with its unusual regulatory properties, seems to play a major role in controlling the flux between the glycolytic nonphosphorylative Entner-Doudoroff and the glycolytic/gluconeogenetic semiphosphorylative Entner-Doudoroff pathway | Saccharolobus solfataricus |
2.7.1.165 | metabolism | the enzyme plays a major role in controlling the flux between the glycolytic non-phosphorylative-Entner-Doudoroff and the glycolytic/gluconeogenetic semiphosphorylative Entner-Doudoroff pathway | Saccharolobus solfataricus |
2.7.1.178 | malfunction | a 2-keto-3-deoxygluconate kinase deletion strain reveals no difference in glycolytic growth compared with the reference strain | Saccharolobus solfataricus |