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Literature summary extracted from
- Hydrogen production from pyruvate by enzymes purified from the hyperthermophilic archaeon, Pyrococcus furiosus: A key role for NADPH (1994), FEMS Microbiol. Lett., 122, 245-250.
No PubMed abstract available
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.12.98.4 | 0.04 | - |
NADP+ | at pH 8.0, 80°C | Pyrococcus furiosus |  |
| 1.12.98.4 | 0.2 | - |
NADPH | at pH 8.0, 80°C | Pyrococcus furiosus | |
| 1.12.98.4 | 3 | - |
NADH | at pH 8.0, 80°C | Pyrococcus furiosus | |
| 1.12.98.4 | 5 | - |
methyl viologen | at pH 8.0, 80°C | Pyrococcus furiosus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.12.98.4 | Ni2+ | contains nickel | Pyrococcus furiosus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.12.98.4 | Pyrococcus furiosus | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.12.98.4 | H2 + methyl viologen | - |
Pyrococcus furiosus | H2S + reduced methyl viologen | - |
r | |
| 1.12.98.4 | H2 + NADP+ | - |
Pyrococcus furiosus | H2S + NADPH + H+ | - |
r | |
| 1.12.98.4 | H2S + NADH + H+ | NADH is a very inefficient electron carrier for sulfhydrogenase | Pyrococcus furiosus | H2 + NAD+ | - |
r | |
| 1.12.98.4 | H2S + NADPH + H+ | - |
Pyrococcus furiosus | H2 + NADP+ | - |
r | |
| 1.12.98.4 | additional information | ferredoxin is not an effective electron donor for Pyrococcus furiosus sulfhydrogenase. NAD+ is not reduced | Pyrococcus furiosus | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.12.98.4 | SH2ase | - |
Pyrococcus furiosus |
| 1.12.98.4 | sulhydrogenase | bifunctional emzyme | Pyrococcus furiosus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.12.98.4 | 90 | - |
optimum temperature is at least 90°C | Pyrococcus furiosus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.12.98.4 | 90 | - |
activity decreases with increasing temperatures above 90°C | Pyrococcus furiosus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.12.98.4 | 8 | - |
about pH 8.0 | Pyrococcus furiosus |
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Release 2023.1 (January 2023)
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