Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kim, S.R.; Kwee, N.R.; Kim, H.; Jin, Y.S.
    Feasibility of xylose fermentation by engineered Saccharomyces cerevisiae overexpressing endogenous aldose reductase (GRE3), xylitol dehydrogenase (XYL2) and xylulokinase (XYL3) from Scheffersomyces stipitis (2013), FEMS Yeast Res., 13, 312-321.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.9 expressed in Saccharomyces cerevisiae strain DX23 Scheffersomyces stipitis
1.1.1.9 recombinant overexpression in Saccharomyces cerevisiae strain D452-2, coexpression with Saccharomyces cerevisiae enzymes/genes xylulokinase XKS1, and xylose reductase XYL1 or XYL3, aldose reductase GRE3. Xylose fermentation efficiency of a strain overexpressing GRE3 is dramatically increased by high expression levels of XYL2 Scheffersomyces stipitis
2.7.1.17 expressed in Saccharomyces cerevisiae strain DX23 Scheffersomyces stipitis

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.9 additional information engineered Saccharomyces cerevisiae strain DGX23 overexpressing aldose reductase GRE3, xylitol dehydrogenase XYL2, and xylulokinase XYL3 can ferment xylose as well as a mixture of glucose and xylose with higher ethanol yields and productivities than those of an isogenic strain overexpressing xylose reductase XYL1, xylitol dehydrogenase XYL2, and xylulokinase XYL3 under oxygen-limited conditions. Optimized expression levels of GRE3, XYL2, and XYL3 can overcome redox imbalance during xylose fermentation by engineered Saccharomyces cerevisiae under oxygen-limited conditions, anaerobic xylose fermentation Scheffersomyces stipitis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.9 xylitol + NAD+ Scheffersomyces stipitis
-
 D-xylulose + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.9 Scheffersomyces stipitis P22144
-
-
1.1.1.9 Scheffersomyces stipitis P22144 i.e Pichia stipitis
-
2.7.1.17 Scheffersomyces stipitis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.9 xylitol + NAD+
-
 Scheffersomyces stipitis D-xylulose + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.9 XYL2
-
 Scheffersomyces stipitis
1.1.1.9 xylitol dehydrogenase
-
 Scheffersomyces stipitis
2.7.1.17 XYL3
-
 Scheffersomyces stipitis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.9 NAD+
-
 Scheffersomyces stipitis
1.1.1.9 NAD+ specific for Scheffersomyces stipitis

General Information

EC Number General Information Comment Organism
2.7.1.17 physiological function engineered Saccharomyces cerevisiae overexpressing GRE3, XYL2, and XYL3 can ferment xylose as well as a mixture of glucose and xylose with higher ethanol yields and productivities than those of an isogenic strain overexpressing XYL1, XYL2, and XYL3 under oxygen-limited conditions Scheffersomyces stipitis