EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.365 | expressed in Escherichia coli DH5alpha cells | Aspergillus niger |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.365 | 0.036 | - |
NADPH | with D-galacturonate as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger | |
1.1.1.365 | 0.175 | - |
D-galacturonate | with NADPH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger | |
1.1.1.365 | 0.326 | - |
NADH | with D-galacturonate as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger | |
1.1.1.365 | 7.11 | - |
D-galacturonate | with NADH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.365 | 44000 | - |
x * 44000, His6-tagged enzyme, SDS-PAGE | Aspergillus niger |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.365 | D-galacturonate + NADPH + H+ | Aspergillus niger | - |
L-galactonate + NADP+ | - |
? | |
1.1.1.365 | D-galacturonate + NADPH + H+ | Aspergillus niger CBS 120.49 | - |
L-galactonate + NADP+ | - |
? | |
1.1.1.372 | L-glyceraldehyde + NADPH + H+ | Aspergillus niger | - |
glycerol + NADP+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.365 | Aspergillus niger | - |
- |
- |
1.1.1.365 | Aspergillus niger CBS 120.49 | - |
- |
- |
1.1.1.372 | Aspergillus niger | - |
- |
- |
4.1.2.54 | Aspergillus niger | A8DRH7 | - |
- |
4.1.2.54 | Aspergillus niger CBS 120.49 | A8DRH7 | - |
- |
4.1.2.54 | Trichoderma reesei | A6Y9S5 | - |
- |
4.1.2.54 | Trichoderma reesei CBS 383.78 (QM6a) | A6Y9S5 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.365 | mycelium | - |
Aspergillus niger | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.365 | 0.12 | - |
with NAD+ and L-galactonate as substrates, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger |
1.1.1.365 | 0.17 | - |
with NADH and D-glucuronate as substrates, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger |
1.1.1.365 | 0.21 | - |
with NADP+ and L-galactonate as substrates, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger |
1.1.1.365 | 3.9 | - |
with NADPH and D-glucuronate as substrates, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger |
1.1.1.365 | 6.9 | - |
with NADH and D-galacturonate as substrates, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger |
1.1.1.365 | 8.7 | - |
with NADPH and D-galacturonate as substrates, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.365 | D-galacturonate + NADH + H+ | - |
Aspergillus niger | L-galactonate + NAD+ | - |
r | |
1.1.1.365 | D-galacturonate + NADH + H+ | - |
Aspergillus niger CBS 120.49 | L-galactonate + NAD+ | - |
r | |
1.1.1.365 | D-galacturonate + NADPH + H+ | - |
Aspergillus niger | L-galactonate + NADP+ | - |
? | |
1.1.1.365 | D-galacturonate + NADPH + H+ | - |
Aspergillus niger | L-galactonate + NADP+ | - |
r | |
1.1.1.365 | D-galacturonate + NADPH + H+ | - |
Aspergillus niger CBS 120.49 | L-galactonate + NADP+ | - |
? | |
1.1.1.365 | D-galacturonate + NADPH + H+ | - |
Aspergillus niger CBS 120.49 | L-galactonate + NADP+ | - |
r | |
1.1.1.365 | D-glucuronate + NADH + H+ | - |
Aspergillus niger | ? | - |
? | |
1.1.1.365 | D-glucuronate + NADH + H+ | - |
Aspergillus niger CBS 120.49 | ? | - |
? | |
1.1.1.365 | D-glucuronate + NADPH + H+ | - |
Aspergillus niger | ? | - |
? | |
1.1.1.365 | D-glucuronate + NADPH + H+ | - |
Aspergillus niger CBS 120.49 | ? | - |
? | |
1.1.1.365 | L-galactonate + NAD+ | - |
Aspergillus niger | D-galacturonate + NADH + H+ | - |
r | |
1.1.1.365 | L-galactonate + NADP+ | - |
Aspergillus niger | D-galacturonate + NADPH + H+ | - |
r | |
1.1.1.372 | L-glyceraldehyde + NADPH + H+ | - |
Aspergillus niger | glycerol + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.365 | ? | x * 44000, His6-tagged enzyme, SDS-PAGE | Aspergillus niger |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.365 | gaaA | - |
Aspergillus niger |
1.1.1.372 | gaaD | - |
Aspergillus niger |
1.1.1.372 | L-glyceraldehyde reductase | - |
Aspergillus niger |
4.1.2.54 | GAAC | - |
Aspergillus niger |
4.1.2.54 | Kdg1 | - |
Trichoderma reesei |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.365 | 0.08 | - |
L-galactonate | with NAD+ as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger | |
1.1.1.365 | 0.13 | - |
D-glucuronate | with NADH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger | |
1.1.1.365 | 0.16 | - |
L-galactonate | with NADP+ as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger | |
1.1.1.365 | 3.11 | - |
D-glucuronate | with NADPH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger | |
1.1.1.365 | 4.98 | - |
NADH | with D-galacturonate as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger | |
1.1.1.365 | 5.45 | - |
D-galacturonate | with NADH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger | |
1.1.1.365 | 6.88 | - |
D-galacturonate | with NADPH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger | |
1.1.1.365 | 7.11 | - |
NADPH | with D-galacturonate as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.365 | NADH | - |
Aspergillus niger | |
1.1.1.365 | NADPH | preferred cofactor | Aspergillus niger | |
1.1.1.372 | NADPH | - |
Aspergillus niger |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.365 | 0.767 | - |
D-galacturonate | with NADH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger | |
1.1.1.365 | 15.72 | - |
NADH | with D-galacturonate as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger | |
1.1.1.365 | 39.29 | - |
D-galacturonate | with NADPH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger | |
1.1.1.365 | 197.2 | - |
NADPH | with D-galacturonate as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger |