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Literature summary extracted from

  • Girfoglio, M.; Rossi, M.; Cannio, R.
    Cellulose degradation by Sulfolobus solfataricus requires a cell-anchored endo-beta-1-4-glucanase (2012), J. Bacteriol., 194, 5091-5100.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 cloning and expression of the sso1354 gene in different hosts, Escherichia coli and Sulfolobus solfataricus itself Saccharolobus solfataricus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.4 extracellular
-
 Saccharolobus solfataricus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 35154
-
 x * 35154, enzyme recombinantly expressed in Escherichia coli, calculated from sequence Saccharolobus solfataricus
3.2.1.4 35500
-
 x * 35500, enzyme recombinantly expressed in Escherichia coli, SDS-PAGE Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Saccharolobus solfataricus Q97YG7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 additional information
-
 pH 5.0, specific activity of the enzyme recombinantly expressed in Sulfolobus solfataricus is about 200fold higher compared to the specific activity of the enzyme recombinantly expressed in Escherichia coli Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethyl-cellulose + H2O
-
 Saccharolobus solfataricus ?
-
 ?
3.2.1.4 cellulose + H2O
-
 Saccharolobus solfataricus ?
-
 ?
3.2.1.4 curdlan + H2O activity is 26% compared to activity with carboxymethyl-cellulose Saccharolobus solfataricus ?
-
 ?
3.2.1.4 debranched arabinan activity is 25% compared to activity with carboxymethyl-cellulose Saccharolobus solfataricus ?
-
 ?
3.2.1.4 lichenan + H2O activity is 120% compared to activity with carboxymethyl-cellulose Saccharolobus solfataricus ?
-
 ?
3.2.1.4 additional information no hydrolysis of p-nitrophenyl-beta-D-cellobioside and p-nitrophenyl-beta-D-cellotrioside, no exocellulase activity Saccharolobus solfataricus ?
-
 ?
3.2.1.4 oat spelt xylan + H2O activity is 25% compared to activity with carboxymethyl-cellulose Saccharolobus solfataricus ?
-
 ?
3.2.1.4 pachyman + H2O activity is 22% compared to activity with carboxymethyl-cellulose Saccharolobus solfataricus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 35154, enzyme recombinantly expressed in Escherichia coli, calculated from sequence Saccharolobus solfataricus
3.2.1.4 ? x * 35500, enzyme recombinantly expressed in Escherichia coli, SDS-PAGE Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 SSO1354 enzyme
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 90
-
 enzyme recombinantly expressed in Sulfolobus solfataricus Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 90
-
 half-life: 180 min Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 4
-
 recombinant enzyme from Sulfolobus solfataricus Saccharolobus solfataricus

General Information

EC Number General Information Comment Organism
3.2.1.4 physiological function the enzyme is responsible for the main cellulase activity of Sulfolobus solfataricus Saccharolobus solfataricus