EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.2.54 | expression in Saccharomyces cerevisiae as a histidine-tagged protein | Trichoderma reesei |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.2.54 | D-glyceraldehyde | inhibits reverse reaction with pyruvate and L-glyceraldehyde | Trichoderma reesei |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.2.54 | 0.5 | - |
pyruvate | pH 7.0, 30°C | Trichoderma reesei | |
4.1.2.54 | 1.2 | - |
L-glyceraldehyde | pH 7.0, 30°C | Trichoderma reesei | |
4.1.2.54 | 3.5 | - |
2-dehydro-3-deoxy-L-galactonate | pH 7.0, 30°C | Trichoderma reesei |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.2.54 | 33407 | - |
x * 33407, calculated from sequence | Trichoderma reesei |
4.1.2.54 | 37000 | - |
x * 37000, SDS-PAGE | Trichoderma reesei |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.2.54 | 2-dehydro-3-deoxy-L-galactonate | Trichoderma reesei | the enzyme is part of the fungal pathway of D-galacturonate degradation | pyruvate + L-glyceraldehyde | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.2.54 | Trichoderma reesei | A6Y9S5 | i.e. Trichoderma reesei | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.2.54 | - |
Trichoderma reesei |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.1.2.54 | mycelium | the L-threo-3-deoxy-hexulosonate aldolase activity is induced during growth on D-galacturonate | Trichoderma reesei | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.2.54 | 2-dehydro-3-deoxy-L-galactonate | the enzyme is part of the fungal pathway of D-galacturonate degradation | Trichoderma reesei | pyruvate + L-glyceraldehyde | - |
r | |
4.1.2.54 | 2-dehydro-3-deoxy-L-galactonate | the reverse reaction is significantly stronger with L-glyceraldehyde as a substrate than with D-glyceraldehyde. No activity with D-threo-3-deoxy-hexulosonate | Trichoderma reesei | pyruvate + L-glyceraldehyde | - |
r | |
4.1.2.54 | additional information | the reaction with D-glyceraldehyde, dihydroxyacetone, diacetyl, acetone, glyoxal, acetaldehyde or formaldehyde plus pyruvate is below the detection limit | Trichoderma reesei | ? | - |
? | |
4.1.2.54 | pyruvate + glycolaldehyde | - |
Trichoderma reesei | ? | - |
? | |
4.1.2.54 | pyruvate + L-glyceraldehyde | - |
Trichoderma reesei | 2-dehydro-3-deoxy-L-galactonate | - |
r | |
4.1.2.54 | pyruvate + methylglyoxal | - |
Trichoderma reesei | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.2.54 | ? | x * 37000, SDS-PAGE | Trichoderma reesei |
4.1.2.54 | ? | x * 33407, calculated from sequence | Trichoderma reesei |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.2.54 | LGA1 | - |
Trichoderma reesei |
EC Number | Organism | Comment | Expression |
---|---|---|---|
4.1.2.54 | Trichoderma reesei | induced in Hypocrea jecorina during growth on D-galacturonate | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.1.2.54 | malfunction | the deletion of the gene results in a strain unable to grow on D-galacturonate and accumulating L-threo-3-deoxy-hexulosonate | Trichoderma reesei |
4.1.2.54 | physiological function | the enzyme is induced during growth on D-galacturonate | Trichoderma reesei |