EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.20 | synthesis | the enzyme can be potentially useful for starch hydrolysis as well for the novel synthesis of oligosaccharides in industry | Sulfurisphaera tokodaii |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.20 | expressed as a fusion protein in Escherichia coli | Sulfurisphaera tokodaii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.20 | 2.33 | - |
maltopentaose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
3.2.1.20 | 2.78 | - |
maltotetraose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
3.2.1.20 | 3.11 | - |
maltotriose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
3.2.1.20 | 3.18 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
3.2.1.20 | 4.4 | - |
maltohexaose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
3.2.1.20 | 5.09 | - |
maltoheptaose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
3.2.1.20 | 5.78 | - |
panose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
3.2.1.20 | 10.9 | - |
maltose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
3.2.1.20 | 17.3 | - |
isomaltose | pH 4.0, 80°C | Sulfurisphaera tokodaii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 70500 | - |
x * 70500, SDS-PAGE | Sulfurisphaera tokodaii |
3.2.1.20 | 75600 | - |
x * 75600, calculated from sequence | Sulfurisphaera tokodaii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.20 | Sulfurisphaera tokodaii | F9VPH0 | - |
- |
3.2.1.20 | Sulfurisphaera tokodaii 7 | F9VPH0 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.20 | - |
Sulfurisphaera tokodaii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 12.7 | - |
pH 4.0, 80°C | Sulfurisphaera tokodaii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.20 | 4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Sulfurisphaera tokodaii | 4-nitrophenol + alpha-D-glucopyranose | - |
? | |
3.2.1.20 | isomaltose + H2O | - |
Sulfurisphaera tokodaii | ? | - |
? | |
3.2.1.20 | maltoheptaose + H2O | - |
Sulfurisphaera tokodaii | ? | - |
? | |
3.2.1.20 | maltoheptaose + H2O | - |
Sulfurisphaera tokodaii 7 | ? | - |
? | |
3.2.1.20 | maltohexaose + H2O | - |
Sulfurisphaera tokodaii | ? | - |
? | |
3.2.1.20 | maltohexaose + H2O | - |
Sulfurisphaera tokodaii 7 | ? | - |
? | |
3.2.1.20 | maltopentaose + H2O | - |
Sulfurisphaera tokodaii | ? | - |
? | |
3.2.1.20 | maltopentaose + H2O | - |
Sulfurisphaera tokodaii 7 | ? | - |
? | |
3.2.1.20 | maltose + H2O | - |
Sulfurisphaera tokodaii | 2 D-glucose | - |
? | |
3.2.1.20 | maltotetraose + H2O | - |
Sulfurisphaera tokodaii | ? | - |
? | |
3.2.1.20 | maltotetraose + H2O | - |
Sulfurisphaera tokodaii 7 | ? | - |
? | |
3.2.1.20 | maltotriose + H2O | - |
Sulfurisphaera tokodaii | ? | - |
? | |
3.2.1.20 | additional information | exclusively hydrolyzes alpha-1,4-glycosidic linkages of oligosaccharides in an exo-type manner, with highest catalytic efficiency toward maltotriose. The enzyme also displays transglycosylation activity, converting maltose to isomaltose, panose, maltotriose, isomaltotriose, etc. | Sulfurisphaera tokodaii | ? | - |
? | |
3.2.1.20 | additional information | exclusively hydrolyzes alpha-1,4-glycosidic linkages of oligosaccharides in an exo-type manner, with highest catalytic efficiency toward maltotriose. The enzyme also displays transglycosylation activity, converting maltose to isomaltose, panose, maltotriose, isomaltotriose, etc. | Sulfurisphaera tokodaii 7 | ? | - |
? | |
3.2.1.20 | panose + H2O | - |
Sulfurisphaera tokodaii | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.20 | ? | x * 70500, SDS-PAGE | Sulfurisphaera tokodaii |
3.2.1.20 | ? | x * 75600, calculated from sequence | Sulfurisphaera tokodaii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.20 | st2525 | - |
Sulfurisphaera tokodaii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 80 | - |
assay at | Sulfurisphaera tokodaii |
3.2.1.20 | 95 | - |
- |
Sulfurisphaera tokodaii |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 75 | 110 | 75°C: about 50% of maximal activity, 110°C: about 75% of maximal activity | Sulfurisphaera tokodaii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 95 | - |
half-life: 144 min | Sulfurisphaera tokodaii |
3.2.1.20 | 100 | - |
half-life: 40.1 min | Sulfurisphaera tokodaii |
3.2.1.20 | 105 | - |
half-life: 7.75 min | Sulfurisphaera tokodaii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.20 | 0.0175 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
3.2.1.20 | 0.02 | - |
maltopentaose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
3.2.1.20 | 0.02 | - |
maltohexaose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
3.2.1.20 | 0.02 | - |
maltoheptaose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
3.2.1.20 | 0.02 | - |
isomaltose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
3.2.1.20 | 0.02 | - |
panose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
3.2.1.20 | 0.03 | - |
maltotetraose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
3.2.1.20 | 0.04 | - |
maltotriose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
3.2.1.20 | 0.11 | - |
maltose | pH 4.0, 80°C | Sulfurisphaera tokodaii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 4 | - |
- |
Sulfurisphaera tokodaii |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 3.5 | 5 | pH 3.5: about 95% of maximal activity, pH 5.0: about 70% of maximal activity, potassium phosphate buffer | Sulfurisphaera tokodaii |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 4 | 11 | 4°C, 30 min, the enzyme retains at least 85% of its maximal activity | Sulfurisphaera tokodaii |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.20 | 0.0013 | - |
isomaltose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
3.2.1.20 | 0.0034 | - |
panose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
3.2.1.20 | 0.0038 | - |
maltoheptaose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
3.2.1.20 | 0.0052 | - |
maltohexaose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
3.2.1.20 | 0.0055 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
3.2.1.20 | 0.0097 | - |
maltose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
3.2.1.20 | 0.0098 | - |
maltopentaose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
3.2.1.20 | 0.0113 | - |
maltotetraose | pH 4.0, 80°C | Sulfurisphaera tokodaii | |
3.2.1.20 | 0.0136 | - |
maltotriose | pH 4.0, 80°C | Sulfurisphaera tokodaii |