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Literature summary extracted from

  • Mandal, S.; Bose, P.; Dawar, S.; Rajarani, A.; Santha, I.
    Kinetic and expression profiling of cytosolic pyruvate kinase enzyme during seed development of Indian mustard (Brassica juncea) (2011), J. Plant Biochem. Biotechnol., 20, 102-109.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.40 expression profiling by RT-PCR techniques Brassica juncea

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 3-phosphoglycerate 8% inhibition at 5 mM Brassica juncea
2.7.1.40 AMP 11% inhibition at 5 mM Brassica juncea
2.7.1.40 ATP 48% inhibition at 5 mM, significant allosteric effector of the partially purified enzyme Brassica juncea
2.7.1.40 citrate 60% inhibition at 5 mM, significant allosteric effector of the partially purified enzyme Brassica juncea
2.7.1.40 isocitrate 30% inhibition at 5 mM Brassica juncea
2.7.1.40 additional information poor inhibition of glucose 1-phosphate and fumaric acid at 5 mM Brassica juncea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.40 additional information
-
 additional information kinetics, in presence of saturated substrate concentration, the enzyme exhibits hyperbolic kinetics for both ADP and PEP Brassica juncea
2.7.1.40 0.0965
-
 ADP pH 7.0, 25°C Brassica juncea
2.7.1.40 0.1785
-
 phosphoenolpyruvate pH 7.0, 25°C Brassica juncea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.40 chloroplast plastidial isozyme Brassica juncea 9507
-
2.7.1.40 cytoplasm cytosolic isozyme Brassica juncea 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.40 Mg2+ required Brassica juncea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.40 ATP + pyruvate Brassica juncea
-
 ADP + phosphoenolpyruvate
-
 r
2.7.1.40 ATP + pyruvate Brassica juncea Pusa Bold
-
 ADP + phosphoenolpyruvate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Brassica juncea
-
-
-
2.7.1.40 Brassica juncea Pusa Bold
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40 native cytosolic isozyme 12.3fold from seeds by anion exchange and hydroxyapatite chromatography, inactivation of plastidial isoform by heating the enzyme extract at 60°C for 3 min Brassica juncea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.40 flower
-
 Brassica juncea
-
2.7.1.40 leaf
-
 Brassica juncea
-
2.7.1.40 seed expression profiling of cytosolic pyruvate kinase enzyme during seed development, enzyme activity is highest during middle stage, overview Brassica juncea
-
2.7.1.40 seedling highest activity Brassica juncea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.40 690.7
-
 partially purified cytosolic isozyme, pH 7.0, 25°C Brassica juncea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ATP + pyruvate
-
 Brassica juncea ADP + phosphoenolpyruvate
-
 r
2.7.1.40 ATP + pyruvate
-
 Brassica juncea Pusa Bold ADP + phosphoenolpyruvate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.7.1.40 PYK
-
 Brassica juncea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.40 25
-
 assay at Brassica juncea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.40 60
-
 3 min, pH 7.0, plastidial isoform, inactivation Brassica juncea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.40 7
-
 narrow optimum, cytosolic isozyme Brassica juncea
2.7.1.40 8
-
 plastidial isozyme Brassica juncea

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.40 ATP
-
 Brassica juncea

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.1.40 3.64
-
 pH 7.0, 25°C Brassica juncea citrate
2.7.1.40 5
-
 pH 7.0, 25°C Brassica juncea ATP

General Information

EC Number General Information Comment Organism
2.7.1.40 metabolism pyruvate kinase is a crucial regulatory enzyme involved in glycolysis Brassica juncea