Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Knaevelsrud, I.; Ruoff, P.; Anensen, H.; Klungland, A.; Bjelland, S.; Birkeland, N.K.
    Excision of uracil from DNA by the hyperthermophilic Afung protein is dependent on the opposite base and stimulated by heat-induced transition to a more open structure (2001), Mutat. Res., 487, 173-190.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.27 overexpression in Escherichia coli Archaeoglobus fulgidus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.27 4-hydroxymercuribenzoate 1 mM, 94% inhibition Archaeoglobus fulgidus
3.2.2.27 Uracil 5 mM, 51% inhibition Archaeoglobus fulgidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.27 0.000536
-
 uracil-mismatched single-stranded DNA pH 7.5, 95°C Archaeoglobus fulgidus
3.2.2.27 0.00134
-
 uracil-mismatched double-stranded DNA pH 7.5, 70°C Archaeoglobus fulgidus
3.2.2.27 0.00151
-
 uracil-mismatched double-stranded DNA pH 7.5, 80°C Archaeoglobus fulgidus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.27 27000
-
 gel filtration Archaeoglobus fulgidus

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.27 Archaeoglobus fulgidus O28007
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.27
-
 Archaeoglobus fulgidus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.27 0.009
-
 pH 7.5, 80°C Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.27 additional information the protein is devoid of methyl purine and formamidopyrimidine DNA glycosylase activity, respectively Archaeoglobus fulgidus ?
-
 ?
3.2.2.27 uracil-mismatched double-stranded DNA + H2O the enzyme exhibits opposite base-dependent excision of uracil in order of decreasing efficiency: uridine, U:T, U:C, U:G, U:A Archaeoglobus fulgidus uracil + double-stranded DNA with abasic site
-
 ?
3.2.2.27 uracil-mismatched single-stranded DNA + H2O
-
 Archaeoglobus fulgidus uracil + single-stranded DNA with abasic site
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.27 Afung protein
-
 Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.27 80 90
-
 Archaeoglobus fulgidus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.2.27 20 50 fluorescence and activity measurements following incubation at different temperatures suggest the following model describing structure-activity relationships: at temperatures from 20 to 50°C the protein exists as a compact protein exhibiting low enzyme activity, whereas at temperatures above 50°C, the Afung conformation opens up, which is associated with the acquisition of high enzyme activity Archaeoglobus fulgidus
3.2.2.27 65 100 65°C: about 55% of maximal activity, 100°C: about 50% of maximal activity Archaeoglobus fulgidus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.2.27 70
-
 20 min, enzyme in crude protein extract is stable Archaeoglobus fulgidus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.2.27 additional information
-
 additional information kinetic parameters determined for Afung protein suggest a significantly lower level of enzymatic uracil release in Aeroglobus fulgidus as compared to the mesophilic Escherichia coli Archaeoglobus fulgidus
3.2.2.27 0.0175
-
 uracil-mismatched double-stranded DNA pH 7.5, 70°C Archaeoglobus fulgidus
3.2.2.27 0.02
-
 uracil-mismatched double-stranded DNA pH 7.5, 80°C Archaeoglobus fulgidus
3.2.2.27 0.083
-
 uracil-mismatched single-stranded DNA pH 7.5, 95°C Archaeoglobus fulgidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.27 4.8
-
-
 Archaeoglobus fulgidus
3.2.2.27 7.5
-
 assay at Archaeoglobus fulgidus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.2.27 4 7 pH 4.0: about 25% of maximal activity, pH 7.0: about 25% of maximal activity Archaeoglobus fulgidus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.2.27 Archaeoglobus fulgidus isoelectric focusing
-
 5.7

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.2.27 13.1
-
 uracil-mismatched double-stranded DNA pH 7.5, 70°C Archaeoglobus fulgidus
3.2.2.27 13.2
-
 uracil-mismatched double-stranded DNA pH 7.5, 80°C Archaeoglobus fulgidus
3.2.2.27 154.9
-
 uracil-mismatched single-stranded DNA pH 7.5, 95°C Archaeoglobus fulgidus