EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.241 | DNA and amino acid sequence determination and analysis, Bx7 maps to the cluster of Bx genes on the short arm of chromosome 4, Bx7 is located 9 cM distal to the marker adh2 at position 47, phylogenetic analysis, expression as His-tagged enzyme in Escherichia coli | Zea mays |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.241 | additional information | 1 mM Mg2+, 1 mM EDTA, 5% ethanol, or 6% DMSO have no effect on BX7 activity | Zea mays |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.11.59 | 0.07 | - |
2-oxoglutarate | in 100 mM potassium phosphate buffer (pH 7.0), at 30°C | Zea mays | |
1.14.11.59 | 0.373 | - |
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside | in 100 mM potassium phosphate buffer (pH 7.0), at 30°C | Zea mays | |
2.1.1.241 | additional information | - |
additional information | steady-state kinetic constants of BX7 | Zea mays |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.11.59 | cytoplasm | - |
Zea mays | 5737 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.241 | additional information | Mg2+ is not required for catalysis | Zea mays |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.241 | 40000 | - |
x * 40000, native enzyme, SDS-PAGE | Zea mays |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.241 | S-adenosyl-L-methionine + (2R)-4,7-dihydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside | Zea mays | - |
S-adenosyl-L-homocysteine + (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.11.59 | Zea mays | - |
- |
- |
2.1.1.241 | Zea mays | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.241 | recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Zea mays |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.11.59 | seedling | highest expression | Zea mays | - |
2.1.1.241 | seedling | Bx7 is highest expressed in seedling tissue | Zea mays | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.11.59 | (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + 2-oxoglutarate + O2 | - |
Zea mays | (2R)-4,7-dihydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + succinate + CO2 + H2O | - |
? | |
1.14.11.59 | additional information | 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one is not hydroxylated by the enzyme | Zea mays | ? | - |
? | |
2.1.1.241 | additional information | the only substance that serves as a substrate for the recombinant Bx7 is TRIBOA-Glc, neither the aglucone TRIBOA nor well-known substrates of plant O-methyltransferases are efficiently converted by the enzyme. In case of quercetin, a minor activity is detected. BX7 has no catalytic activity toward phenylpropanoids and flavonoids, e.g. apigenin is no substrate | Zea mays | ? | - |
? | |
2.1.1.241 | S-adenosyl-L-methionine + (2R)-4,7-dihydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside | - |
Zea mays | S-adenosyl-L-homocysteine + (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside | - |
? | |
2.1.1.241 | S-adenosyl-L-methionine + (2R)-4,7-dihydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside | conversion of (2R)-2,4,7-trihydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside, TRIBOA-Glc, to DIMBOA-Glc | Zea mays | S-adenosyl-L-homocysteine + (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside | i.e. DIMBOA-Glc | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.241 | ? | x * 40000, native enzyme, SDS-PAGE | Zea mays |
2.1.1.241 | More | peptide mapping by mass spectrometry | Zea mays |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.11.59 | benzoxazinless6 | - |
Zea mays |
1.14.11.59 | BX6 | - |
Zea mays |
1.14.11.59 | DIBOA-Glc dioxygenase | - |
Zea mays |
2.1.1.241 | BENZOXAZINLESS7 | - |
Zea mays |
2.1.1.241 | BX7 | - |
Zea mays |
2.1.1.241 | TRIBOA-OMT | - |
Zea mays |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.11.59 | 2.1 | - |
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside | in 100 mM potassium phosphate buffer (pH 7.0), at 30°C | Zea mays |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.11.59 | 6 | - |
- |
Zea mays |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.241 | S-adenosyl-L-methionine | - |
Zea mays |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.1.1.241 | metabolism | Bx7 catalyzes the last step in benzoxazinoid biosynthesis of maize | Zea mays |
2.1.1.241 | physiological function | Bx7 is required for biosynthesis of the benzoxazinoid pesticide 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one, i.e. DIMBOA, that is synthesized in seedlings and stored as glucoside in maize | Zea mays |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.11.59 | 5.6 | - |
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside | in 100 mM potassium phosphate buffer (pH 7.0), at 30°C | Zea mays |