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Literature summary extracted from

  • Zhao, N.; Ferrer, J.L.; Ross, J.; Guan, J.; Yang, Y.; Pichersky, E.; Noel, J.P.; Chen, F.
    Structural, biochemical, and phylogenetic analyses suggest that indole-3-acetic acid methyltransferase is an evolutionarily ancient member of the SABATH family (2008), Plant Physiol., 146, 455-467.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.278 expression in Escherichia coli Oryza sativa Japonica Group

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.278 the crystal structure of the enzyme is determined and refined to 2.75 A resolution Arabidopsis thaliana
2.1.1.278 the crystal structure of the enzyme is determined and refined to 2.75 A resolution Oryza sativa Japonica Group

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.278 Ca2+ 5 mM, 15% inhibition Oryza sativa Japonica Group
2.1.1.278 Cu2+ 5 mM, more than 95% inhibition Oryza sativa Japonica Group
2.1.1.278 Fe2+ 5 mM, more than 95% inhibition Oryza sativa Japonica Group
2.1.1.278 Fe3+ 5 mM, more than 95% inhibition Oryza sativa Japonica Group
2.1.1.278 Mg2+ 5 mM, 15% inhibition Oryza sativa Japonica Group
2.1.1.278 Mn2+ 5 mM, more than 95% inhibition Oryza sativa Japonica Group
2.1.1.278 Zn2+ 5 mM, more than 95% inhibition Oryza sativa Japonica Group

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.278 0.0073
-
 S-adenosyl-L-methionine pH 7.5, 25°C Oryza sativa Japonica Group
2.1.1.278 0.0179
-
 (indol-3-yl)acetate pH 7.5, 25°C Oryza sativa Japonica Group

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.278 K+ 5 mM, more than 1.5fold stimulation Oryza sativa Japonica Group
2.1.1.278 Na+ 5 mM, more than 1.5fold stimulation Oryza sativa Japonica Group
2.1.1.278 NH4+ 5 mM, more than 1.5fold stimulation Oryza sativa Japonica Group

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.278 43800
-
 x * 43800, calculated from sequence Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.278 S-adenosyl-L-methionine + (indol-3-yl)acetate Arabidopsis thaliana the enzyme modulates homeostasis of (indol-3-yl)acetate in plant tissues through methylation of the free carboxyl group of (indol-3-yl)acetate S-adenosyl-L-homocysteine + methyl (indol-3-yl)acetate
-
 ?
2.1.1.278 S-adenosyl-L-methionine + (indol-3-yl)acetate Oryza sativa Japonica Group the enzyme modulates homeostasis of (indol-3-yl)acetate in plant tissues through methylation of the free carboxyl group of (indol-3-yl)acetate S-adenosyl-L-homocysteine + methyl (indol-3-yl)acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.278 Arabidopsis thaliana Q9FLN8
-
-
2.1.1.278 Oryza sativa Japonica Group Q0J998
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.278
-
 Oryza sativa Japonica Group

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.278 additional information no activity with: salicylate, jasmonic acid, farnesoic acid and gibberellic acid Oryza sativa Japonica Group ?
-
 ?
2.1.1.278 S-adenosyl-L-methionine + (indol-3-yl)acetate
-
 Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl (indol-3-yl)acetate
-
 ?
2.1.1.278 S-adenosyl-L-methionine + (indol-3-yl)acetate
-
 Oryza sativa Japonica Group S-adenosyl-L-homocysteine + methyl (indol-3-yl)acetate
-
 ?
2.1.1.278 S-adenosyl-L-methionine + (indol-3-yl)acetate the enzyme modulates homeostasis of (indol-3-yl)acetate in plant tissues through methylation of the free carboxyl group of (indol-3-yl)acetate Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl (indol-3-yl)acetate
-
 ?
2.1.1.278 S-adenosyl-L-methionine + (indol-3-yl)acetate the enzyme modulates homeostasis of (indol-3-yl)acetate in plant tissues through methylation of the free carboxyl group of (indol-3-yl)acetate Oryza sativa Japonica Group S-adenosyl-L-homocysteine + methyl (indol-3-yl)acetate
-
 ?
2.1.1.278 S-adenosyl-L-methionine + (indol-3-yl)butyrate at 2% of the catalytic activity compared to (indol-3-yl)acetate Oryza sativa Japonica Group S-adenosyl-L-homocysteine + methyl (indol-3-yl)butyrate
-
 ?
2.1.1.278 S-adenosyl-L-methionine + dichlorophenoxyacetic acid at 5% of the catalytic activity compared to (indol-3-yl)acetate Oryza sativa Japonica Group S-adenosyl-L-homocysteine + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.278 ? x * 43800, calculated from sequence Oryza sativa Japonica Group

Synonyms

EC Number Synonyms Comment Organism
2.1.1.278 AtIAMT1
-
 Arabidopsis thaliana
2.1.1.278 IAA methyltransferase
-
 Arabidopsis thaliana
2.1.1.278 IAA methyltransferase
-
 Oryza sativa Japonica Group
2.1.1.278 indole-3-acetic acid methyltransferase
-
 Arabidopsis thaliana
2.1.1.278 indole-3-acetic acid methyltransferase
-
 Oryza sativa Japonica Group
2.1.1.278 OsIAMT1
-
 Oryza sativa Japonica Group
2.1.1.278 OsSABATH4
-
 Oryza sativa Japonica Group

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.278 0.025
-
 S-adenosyl-L-methionine pH 7.5, 25°C Oryza sativa Japonica Group
2.1.1.278 0.025
-
 (indol-3-yl)acetate pH 7.5, 25°C Oryza sativa Japonica Group

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.278 7.5
-
-
 Oryza sativa Japonica Group

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.278 6.5 9 pH 6.5: 20% of maximal activity, pH 9.0: 30% of maximal activity Oryza sativa Japonica Group