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Literature summary extracted from

  • Mehere, P.; Han, Q.; Lemkul, J.A.; Vavricka, C.J.; Robinson, H.; Bevan, D.R.; Li, J.
    Tyrosine aminotransferase: biochemical and structural properties and molecular dynamics simulations (2010), Protein Cell, 1, 1023-1032.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.5 expressed in Escherichia coli Mus musculus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.5 through macromolecular crystallography the mTAT crystal structure is determined at 2.9 Å resolution. The crystal structure reveal the interaction between the pyridoxal-5'-phosphate cofactor and the enzyme, as well as the formation of a disulphide bond Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.5 0.7
-
4-hydroxyphenylpyruvate pH 7.0, 38°C Mus musculus
2.6.1.5 0.9
-
phenylpyruvate pH 7.0, 38°C Mus musculus
2.6.1.5 1.8
-
2-oxoglutarate pH 7.0, 38°C Mus musculus
2.6.1.5 1.8
-
L-tyrosine pH 7.0, 38°C Mus musculus
2.6.1.5 4.9
-
L-glutamate pH 7.0, 38°C Mus musculus
2.6.1.5 7.3
-
2-Oxohexanoate pH 7.0, 38°C Mus musculus
2.6.1.5 11.4
-
L-phenylalanine pH 7.0, 38°C Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.5 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.5 using affinity, Source-Q and gel-filtration chromatography Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.5 L-glutamate + 4-hydroxyphenylpyruvate
-
Mus musculus L-tyrosine + 2-oxoglutarate
-
?
2.6.1.5 L-phenylalanine + 2-oxoglutarate
-
Mus musculus 2-oxo-3-phenylpropanoate + L-glutamate
-
?
2.6.1.5 L-tyrosine + 2-oxoglutarate
-
Mus musculus 3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
?
2.6.1.5 L-tyrosine + 2-oxohexanoate
-
Mus musculus 3-(4-hydroxyphenyl)-2-oxopropanoate + (2S)-2-aminohexanoate
-
?
2.6.1.5 L-tyrosine + phenylpyruvate
-
Mus musculus 3-(4-hydroxyphenyl)-2-oxopropanoate + L-phenylalanine
-
?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.5 mTAT
-
Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.5 38
-
assay at Mus musculus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.6.1.5 50 70 highest activity Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.5 1.83
-
2-Oxohexanoate pH 7.0, 38°C Mus musculus
2.6.1.5 2.66
-
phenylpyruvate pH 7.0, 38°C Mus musculus
2.6.1.5 9.33
-
L-glutamate pH 7.0, 38°C Mus musculus
2.6.1.5 15.33
-
L-phenylalanine pH 7.0, 38°C Mus musculus
2.6.1.5 15.83
-
4-hydroxyphenylpyruvate pH 7.0, 38°C Mus musculus
2.6.1.5 19.33
-
L-tyrosine pH 7.0, 38°C Mus musculus
2.6.1.5 25.66
-
2-oxoglutarate pH 7.0, 38°C Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.5 7
-
-
Mus musculus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.6.1.5 0.33
-
2-Oxohexanoate pH 7.0, 38°C Mus musculus
2.6.1.5 1.33
-
L-phenylalanine pH 7.0, 38°C Mus musculus
2.6.1.5 2
-
L-glutamate pH 7.0, 38°C Mus musculus
2.6.1.5 3
-
phenylpyruvate pH 7.0, 38°C Mus musculus
2.6.1.5 10.66
-
L-tyrosine pH 7.0, 38°C Mus musculus
2.6.1.5 14.16
-
2-oxoglutarate pH 7.0, 38°C Mus musculus
2.6.1.5 22.66
-
4-hydroxyphenylpyruvate pH 7.0, 38°C Mus musculus