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Literature summary extracted from

  • Shinmura, K.; Muraki, N.; Yoshida, A.; Hase, T.; Kurisu, G.
    Crystallization and preliminary X-ray studies of an electron-transfer complex of ferredoxin and ferredoxin-dependent glutamate synthase from the cyanobacterium Leptolyngbya boryana (2012), Acta Crystallogr. Sect. F, 68, 324-327.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.7.1 expressed in Escherichia coli JM105 cells Leptolyngbya boryana

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.7.1 hanging drop vapor diffusion method, using 22-23% (w/v) PEG 3350, 170 mM sodium acetate trihydrate pH 7.0, 85 mM Tris-HCl pH 8.2, 15% (v/v) glycerol Leptolyngbya boryana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.7.1 16000
-
 gel filtration Leptolyngbya boryana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.7.1 L-glutamine + 2-oxoglutarate + reduced ferredoxin Leptolyngbya boryana
-
 L-glutamate + oxidized ferredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.7.1 Leptolyngbya boryana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.7.1 ferredoxin-immobilized Sepharose column chromatography Leptolyngbya boryana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.7.1 L-glutamine + 2-oxoglutarate + reduced ferredoxin
-
 Leptolyngbya boryana L-glutamate + oxidized ferredoxin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.7.1 Fd-GOGAT
-
 Leptolyngbya boryana
1.4.7.1 ferredoxin-dependent glutamate synthase
-
 Leptolyngbya boryana
1.4.7.1 glutamine 2-oxoglutarate amidotransferase
-
 Leptolyngbya boryana

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.7.1 FMN
-
 Leptolyngbya boryana
1.4.7.1 [3Fe-4S]-center
-
 Leptolyngbya boryana