BRENDA - Enzyme Database

Genetic modification of Bacillus subtilis for production of D-chiro-inositol, an investigational drug candidate for treatment of type 2 diabetes and polycystic ovary syndrome

Yoshida, K.; Yamaguchi, M.; Morinaga, T.; Ikeuchi, M.; Kinehara, M.; Ashida, H.; Appl. Environ. Microbiol. 72, 1310-1315 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.369
expression in Escherichia coli as a C-terminal His6 tag fusion protein
Bacillus subtilis
5.3.99.11
expressed in Escherichia coli BL21(DE3) cells
Bacillus subtilis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.369
1D-chiro-inositol + NAD+
Bacillus subtilis
the enzyme is part of a myo-inositol/D-chiro-inositol degradation pathway leading to acetyl-CoA
2D-2,3,5/4,6-pentahydroxycyclohexanone + NADH + H+
-
-
r
1.1.1.369
1D-chiro-inositol + NAD+
Bacillus subtilis 168
the enzyme is part of a myo-inositol/D-chiro-inositol degradation pathway leading to acetyl-CoA
2D-2,3,5/4,6-pentahydroxycyclohexanone + NADH + H+
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.369
Bacillus subtilis
P26935
-
-
1.1.1.369
Bacillus subtilis 168
P26935
-
-
5.3.99.11
Bacillus subtilis
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.369
purified as a C-terminal His6 tag fusion protein
Bacillus subtilis
5.3.99.11
-
Bacillus subtilis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.369
1D-chiro-inositol + NAD+
the enzyme is part of a myo-inositol/D-chiro-inositol degradation pathway leading to acetyl-CoA
723995
Bacillus subtilis
2D-2,3,5/4,6-pentahydroxycyclohexanone + NADH + H+
-
-
-
r
1.1.1.369
1D-chiro-inositol + NAD+
1D-chiro-inositol i.e. 1,2,4/3,5,6-cyclohexane-1,2,3,4,5,6-hexol
723995
Bacillus subtilis
2D-2,3,5/4,6-pentahydroxycyclohexanone + NADH + H+
-
-
-
r
1.1.1.369
1D-chiro-inositol + NAD+
the enzyme is part of a myo-inositol/D-chiro-inositol degradation pathway leading to acetyl-CoA
723995
Bacillus subtilis 168
2D-2,3,5/4,6-pentahydroxycyclohexanone + NADH + H+
-
-
-
r
1.1.1.369
1D-chiro-inositol + NAD+
1D-chiro-inositol i.e. 1,2,4/3,5,6-cyclohexane-1,2,3,4,5,6-hexol
723995
Bacillus subtilis 168
2D-2,3,5/4,6-pentahydroxycyclohexanone + NADH + H+
-
-
-
r
5.3.99.11
2-oxo-myo-inositol
-
723995
Bacillus subtilis
1-oxo-D-chiroinositol
-
-
-
r
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.369
expression in Escherichia coli as a C-terminal His6 tag fusion protein
Bacillus subtilis
5.3.99.11
expressed in Escherichia coli BL21(DE3) cells
Bacillus subtilis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.369
1D-chiro-inositol + NAD+
Bacillus subtilis
the enzyme is part of a myo-inositol/D-chiro-inositol degradation pathway leading to acetyl-CoA
2D-2,3,5/4,6-pentahydroxycyclohexanone + NADH + H+
-
-
r
1.1.1.369
1D-chiro-inositol + NAD+
Bacillus subtilis 168
the enzyme is part of a myo-inositol/D-chiro-inositol degradation pathway leading to acetyl-CoA
2D-2,3,5/4,6-pentahydroxycyclohexanone + NADH + H+
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.369
purified as a C-terminal His6 tag fusion protein
Bacillus subtilis
5.3.99.11
-
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.369
1D-chiro-inositol + NAD+
the enzyme is part of a myo-inositol/D-chiro-inositol degradation pathway leading to acetyl-CoA
723995
Bacillus subtilis
2D-2,3,5/4,6-pentahydroxycyclohexanone + NADH + H+
-
-
-
r
1.1.1.369
1D-chiro-inositol + NAD+
1D-chiro-inositol i.e. 1,2,4/3,5,6-cyclohexane-1,2,3,4,5,6-hexol
723995
Bacillus subtilis
2D-2,3,5/4,6-pentahydroxycyclohexanone + NADH + H+
-
-
-
r
1.1.1.369
1D-chiro-inositol + NAD+
the enzyme is part of a myo-inositol/D-chiro-inositol degradation pathway leading to acetyl-CoA
723995
Bacillus subtilis 168
2D-2,3,5/4,6-pentahydroxycyclohexanone + NADH + H+
-
-
-
r
1.1.1.369
1D-chiro-inositol + NAD+
1D-chiro-inositol i.e. 1,2,4/3,5,6-cyclohexane-1,2,3,4,5,6-hexol
723995
Bacillus subtilis 168
2D-2,3,5/4,6-pentahydroxycyclohexanone + NADH + H+
-
-
-
r
5.3.99.11
2-oxo-myo-inositol
-
723995
Bacillus subtilis
1-oxo-D-chiroinositol
-
-
-
r