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Literature summary extracted from
- Cloning, expression and characterization of a eukaryotic cycloalkanone monooxygenase from Cylindrocarpon radicicola ATCC 11011 (2012), Appl. Microbiol. Biotechnol., 94, 705-717.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.13.22 | expression in Escherichia coli | Ilyonectria destructans |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.22 | 0.007 | - |
Cyclobutanone | pH 8.0, 25°C | Ilyonectria destructans |  |
| 1.14.13.22 | 0.04 | - |
cyclohexanone | pH 8.0, 25°C | Ilyonectria destructans | |
| 1.14.13.22 | 0.12 | - |
2-hydroxycyclobutanone | pH 8.0, 25°C | Ilyonectria destructans | |
| 1.14.13.22 | 0.33 | - |
2-phenylcyclohexanone | pH 8.0, 25°C | Ilyonectria destructans | |
| 1.14.13.22 | 0.88 | - |
Cycloheptanone | pH 8.0, 25°C | Ilyonectria destructans | |
| 1.14.13.22 | 1.03 | - |
Cyclooctanone | pH 8.0, 25°C | Ilyonectria destructans | |
| 1.14.13.22 | 4.66 | - |
Cyclopentanone | pH 8.0, 25°C | Ilyonectria destructans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.22 | 54000 | - |
1 * 61900, calculated, 1 * 54000, SDS-PAGE | Ilyonectria destructans |
| 1.14.13.22 | 61900 | - |
1 * 61900, calculated, 1 * 54000, SDS-PAGE | Ilyonectria destructans |
| 1.14.13.22 | 76000 | - |
gel filtration | Ilyonectria destructans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.22 | Ilyonectria destructans | G8H1L8 | - |
- |
| 1.14.13.22 | Ilyonectria destructans ATCC 11011 | G8H1L8 | - |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.22 | 6.8 | - |
substrate cyclohexanone, pH 8.0, 25°C | Ilyonectria destructans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.22 | (1R)-bicyclo[2.2.1]heptane-2,5-dione + NADPH + O2 | 74.4% of the activity with cyclohexanone | Ilyonectria destructans | ? + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 2-hydroxycyclobutanone + NADPH + H+ + O2 | 67.3% of the activity with cyclohexanone | Ilyonectria destructans | 1-oxa-2-oxo-3-hydroxycyclopentane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 2-methyl-cyclohexanone + NADPH + H+ + O2 | 79.2% of the activity with cyclohexanone | Ilyonectria destructans | 1-oxa-2-oxo-3-methylcycloheptane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 2-methyl-cyclohexanone + NADPH + H+ + O2 | 79.2% of the activity with cyclohexanone | Ilyonectria destructans ATCC 11011 | 1-oxa-2-oxo-3-methylcycloheptane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 2-methyl-cyclopentanone + NADPH + H+ + O2 | 60.5% of the activity with cyclohexanone | Ilyonectria destructans | 1-oxa-2-oxo-3-methylcyclohexane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 2-methyl-cyclopentanone + NADPH + H+ + O2 | 60.5% of the activity with cyclohexanone | Ilyonectria destructans ATCC 11011 | 1-oxa-2-oxo-3-methylcyclohexane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | 2-phenylcyclohexanone + NADPH + H+ + O2 | 11% of the activity with cyclohexanone | Ilyonectria destructans | 1-oxa-2-oxo-3-phenylcycloheptane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | bicyclo[3.2.0]hept-2-en-6-one + NADPH + H+ + O2 | 63% of the activity with cyclohexanone | Ilyonectria destructans | 3,3a,6,6a-tetrahydro-1H-cyclopenta[c]furan-1-one + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclobutanone + NADPH + H+ + O2 | 86.9% of the activity with cyclohexanone | Ilyonectria destructans | 1-oxa-2-oxo-cyclopentane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclobutanone + NADPH + H+ + O2 | 86.9% of the activity with cyclohexanone | Ilyonectria destructans ATCC 11011 | 1-oxa-2-oxo-cyclopentane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclobutyl methyl ketone + NADPH + O2 | 17.5% of the activity with cyclohexanone | Ilyonectria destructans | ? + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cycloheptanone + NADPH + H+ + O2 | 23.5% of the activity with cyclohexanone | Ilyonectria destructans | 1-oxa-2-oxo-cyclooctane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cycloheptanone + NADPH + H+ + O2 | 23.5% of the activity with cyclohexanone | Ilyonectria destructans ATCC 11011 | 1-oxa-2-oxo-cyclooctane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + H+ + O2 | best substrate | Ilyonectria destructans | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexanone + NADPH + H+ + O2 | best substrate | Ilyonectria destructans ATCC 11011 | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclohexyl methyl ketone + NADPH + O2 | 15.7% of the activity with cyclohexanone | Ilyonectria destructans | ? + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclooctanone + NADPH + H+ + O2 | 3.1% of the activity with cyclohexanone | Ilyonectria destructans | 1-oxa-2-oxo-cyclononane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | cyclopentanone + NADPH + H+ + O2 | 11.3% of the activity with cyclohexanone | Ilyonectria destructans | 1-oxa-2-oxo-cyclohexane + NADP+ + H2O | - |
? | |
| 1.14.13.22 | norcamphor + NADPH + O2 | 74.8% of the activity with cyclohexanone | Ilyonectria destructans | ? + NADP+ + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.13.22 | monomer | 1 * 61900, calculated, 1 * 54000, SDS-PAGE | Ilyonectria destructans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.13.22 | CAMO | - |
Ilyonectria destructans |
| 1.14.13.22 | cycloalkaone monooxygenase | - |
Ilyonectria destructans |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.22 | 0.68 | - |
Cyclooctanone | pH 8.0, 25°C | Ilyonectria destructans | |
| 1.14.13.22 | 0.73 | - |
2-phenylcyclohexanone | pH 8.0, 25°C | Ilyonectria destructans | |
| 1.14.13.22 | 4.3 | - |
Cycloheptanone | pH 8.0, 25°C | Ilyonectria destructans | |
| 1.14.13.22 | 4.5 | - |
2-hydroxycyclobutanone | pH 8.0, 25°C | Ilyonectria destructans | |
| 1.14.13.22 | 5.9 | - |
Cyclopentanone | pH 8.0, 25°C | Ilyonectria destructans | |
| 1.14.13.22 | 6.8 | - |
Cyclobutanone | pH 8.0, 25°C | Ilyonectria destructans | |
| 1.14.13.22 | 9.8 | - |
cyclohexanone | pH 8.0, 25°C | Ilyonectria destructans | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.22 | additional information | no cofactor: NADH | Ilyonectria destructans | |
| 1.14.13.22 | NADPH | - |
Ilyonectria destructans | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.22 | 0.7 | - |
Cyclooctanone | pH 8.0, 25°C | Ilyonectria destructans | |
| 1.14.13.22 | 1.3 | - |
Cyclopentanone | pH 8.0, 25°C | Ilyonectria destructans | |
| 1.14.13.22 | 2.3 | - |
2-phenylcyclohexanone | pH 8.0, 25°C | Ilyonectria destructans | |
| 1.14.13.22 | 4.9 | - |
Cycloheptanone | pH 8.0, 25°C | Ilyonectria destructans | |
| 1.14.13.22 | 38 | - |
2-hydroxycyclobutanone | pH 8.0, 25°C | Ilyonectria destructans | |
| 1.14.13.22 | 238 | - |
cyclohexanone | pH 8.0, 25°C | Ilyonectria destructans | |
| 1.14.13.22 | 920 | - |
Cyclobutanone | pH 8.0, 25°C | Ilyonectria destructans | |
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