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Literature summary extracted from

  • Meyer, M.; Schweiger, P.; Deppenmeier, U.
    Effects of membrane-bound glucose dehydrogenase overproduction on the respiratory chain of Gluconobacter oxydans (2013), Appl. Microbiol. Biotechnol., 97, 3457-3466.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.5.2 gene gox0265 or mgdh, overexpression of Strep-tagged membrane-bound glucose dehydrogenase from vector pBBR1p452 in membranes of an enzyme-deficient Gluconobacter oxydans strain, that shows 9fold higher enzyme expression level and 5fold increased mGDH activity, and 3fold increased oxygen consumption rates, compared to the wild-type strain, gluconate production and analysis of whole cells, overview Gluconobacter oxydans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.2 5.9
-
 D-glucose recombinant Strep-tagged enzyme, pH 6.0, 25°C Gluconobacter oxydans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.2 membrane the enzyme is a membrane integral quinoprotein Gluconobacter oxydans 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.2 89000
-
 x * 89000, recombinant Strep-tagged enzyme, SDS-PAGE Gluconobacter oxydans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.5.2 D-glucose + ubiquinone Gluconobacter oxydans
-
 D-glucono-1,5-lactone + ubiquinol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.2 Gluconobacter oxydans
-
 gene gox0265 or mgdh
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.2 recombinant Strep-tagged membrane-bound glucose dehydrogenase from from membranes of the expressing Gluconobacter oxydans strain by streptactin affinity chromatography to homogeneity Gluconobacter oxydans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.5.2 additional information
-
 gluconate production and analysis of whole cells Gluconobacter oxydans
1.1.5.2 150
-
 purified recombinant Strep-tagged enzyme, pH 6.0, 25°C Gluconobacter oxydans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.2 2-deoxy-D-glucose + ubiquinone
-
 Gluconobacter oxydans 2-deoxy-D-glucono-1,5-lactone + ubiquinol
-
 ?
1.1.5.2 D-allose + ubiquinone
-
 Gluconobacter oxydans ? + ubiquinol
-
 ?
1.1.5.2 D-fucose + ubiquinone
-
 Gluconobacter oxydans ? + ubiquinol
-
 ?
1.1.5.2 D-glucose + ubiquinone
-
 Gluconobacter oxydans D-glucono-1,5-lactone + ubiquinol
-
 ?
1.1.5.2 D-glucose + ubiquinone preferred substrate, assayed with the electron acceptors phenazine methosulfate and dichlorphenolindophenol Gluconobacter oxydans D-glucono-1,5-lactone + ubiquinol
-
 ?
1.1.5.2 D-xylose + ubiquinone
-
 Gluconobacter oxydans D-xylono-1,5-lactone + ubiquinol
-
 ?
1.1.5.2 additional information the enzyme catalyzes only oxidation of D-aldoses and aldose derivatives Gluconobacter oxydans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.5.2 ? x * 89000, recombinant Strep-tagged enzyme, SDS-PAGE Gluconobacter oxydans

Synonyms

EC Number Synonyms Comment Organism
1.1.5.2 membrane-bound PQQ-dependent glucose dehydrogenase
-
 Gluconobacter oxydans
1.1.5.2 mGDH
-
 Gluconobacter oxydans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.5.2 25
-
 assay at Gluconobacter oxydans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.2 6
-
-
 Gluconobacter oxydans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.2 pyrroloquinoline quinone
-
 Gluconobacter oxydans