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Literature summary extracted from

  • Lindenkamp, N.; Schuermann, M.; Steinbuechel, A.
    A propionate CoA-transferase of Ralstonia eutropha H16 with broad substrate specificity catalyzing the CoA thioester formation of various carboxylic acids (2012), Appl. Microbiol. Biotechnol., 97, 7699-7709.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.3.1 expressed in Escherichia coli BL21(DE3) cells Cupriavidus necator

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.3.1 57000
-
 4 * 57000, calculated from amino acid sequence Cupriavidus necator
2.8.3.1 64000
-
 4 * 64000, SDS-PAGE Cupriavidus necator
2.8.3.1 228000
-
 gel filtration Cupriavidus necator

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.3.1 acetate + propanoyl-CoA Cupriavidus necator
-
 acetyl-CoA + propanoate
-
 ?
2.8.3.1 acetate + propanoyl-CoA Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
 acetyl-CoA + propanoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.1 Cupriavidus necator
-
-
-
2.8.3.1 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.3.1 Ni Sepharose column chromatography Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.1 3-hydroxybutyrate + acetyl-CoA 75% activity compared to propionate Cupriavidus necator acetate + 3-hydroxybutanoyl-CoA
-
 ?
2.8.3.1 3-hydroxybutyrate + acetyl-CoA 75% activity compared to propionate Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 acetate + 3-hydroxybutanoyl-CoA
-
 ?
2.8.3.1 3-hydroxypropanoate + acetyl-CoA 80% activity compared to propionate Cupriavidus necator acetate + 3-hydroxypropanoyl-CoA
-
 ?
2.8.3.1 3-hydroxypropanoate + acetyl-CoA 80% activity compared to propionate Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 acetate + 3-hydroxypropanoyl-CoA
-
 ?
2.8.3.1 4-hydroxybutyrate + acetyl-CoA 19% activity compared to propionate Cupriavidus necator acetate + 4-hydroxybutanoyl-CoA
-
 ?
2.8.3.1 4-hydroxybutyrate + acetyl-CoA 19% activity compared to propionate Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 acetate + 4-hydroxybutanoyl-CoA
-
 ?
2.8.3.1 acetate + propanoyl-CoA
-
 Cupriavidus necator acetyl-CoA + propanoate
-
 ?
2.8.3.1 acetate + propanoyl-CoA
-
 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 acetyl-CoA + propanoate
-
 ?
2.8.3.1 acrylate + acrylyl-CoA 69% activity compared to propionate Cupriavidus necator acetate + propionyl-CoA
-
 ?
2.8.3.1 butyrate + acetyl-CoA 28% activity compared to propionate Cupriavidus necator acetate + butyryl-CoA
-
 ?
2.8.3.1 crotonate + acetyl-CoA 28% activity compared to propionate Cupriavidus necator acetate + crotonyl-CoA
-
 ?
2.8.3.1 lactate + acetyl-CoA 36% activity compared to propionate Cupriavidus necator acetate + lactoyl-CoA
-
 ?
2.8.3.1 propionate + acetyl-CoA propionate is the best substrate, 100% activity Cupriavidus necator acetate + propionyl-CoA
-
 ?
2.8.3.1 succinate + acetyl-CoA 48% activity compared to propionate Cupriavidus necator acetate + succinyl-CoA
-
 ?
2.8.3.1 succinate + acetyl-CoA 48% activity compared to propionate Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 acetate + succinyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.3.1 homotetramer 4 * 64000, SDS-PAGE Cupriavidus necator
2.8.3.1 homotetramer 4 * 57000, calculated from amino acid sequence Cupriavidus necator

Synonyms

EC Number Synonyms Comment Organism
2.8.3.1 PCT
-
 Cupriavidus necator