Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Berk, H.; Thauer, R.K.
    Function of coenzyme F420-dependent NADP reductase in methanogenic archaea containing an NADP-dependent alcohol dehydrogenase (1997), Arch. Microbiol., 168, 396-402.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.40 0.04
-
 reduced coenzyme F420 pH 8.0, 30°C Methanogenium organophilum
1.5.1.40 0.05
-
 NADPH pH 6.0, 30°C Methanogenium organophilum
1.5.1.40 0.1
-
 NADP+ pH 8.0, 30°C Methanogenium organophilum
1.5.1.40 0.1
-
 oxidized coenzyme F420 pH 6.0, 30°C Methanogenium organophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.40 28000
-
 x * 28000, SDS-PAGE Methanogenium organophilum
1.5.1.40 90000
-
 gradient gel electrophoresis under nondenaturating conditions Methanogenium organophilum

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.40 Methanobacterium palustre
-
-
-
1.5.1.40 Methanobacterium palustre DSM 3108
-
-
-
1.5.1.40 Methanoculleus thermophilus
-
-
-
1.5.1.40 Methanoculleus thermophilus DSM 3915
-
-
-
1.5.1.40 Methanofollis liminatans
-
-
-
1.5.1.40 Methanofollis liminatans DSM 4140
-
-
-
1.5.1.40 Methanogenium organophilum P80951
-
-
1.5.1.40 Methanogenium organophilum DSM 3596 P80951
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.5.1.40 stable under oxic conditions Methanogenium organophilum

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.40
-
 Methanogenium organophilum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.40 0.04
-
 pH 8.0, 55°C, cell extract Methanoculleus thermophilus
1.5.1.40 0.06
-
 pH 8.0, 37°C, cell extract Methanofollis liminatans
1.5.1.40 0.3
-
 pH 8.0, 37°C, cell extract Methanobacterium palustre
1.5.1.40 15.1
-
 pH 8.0, 30°C, cell extract Methanogenium organophilum
1.5.1.40 998
-
 pH 8.0, 30°C, purified enzyme Methanogenium organophilum

Storage Stability

EC Number Storage Stability Organism
1.5.1.40 4°C or -20°C, enzyme solution is stable for several weeks Methanogenium organophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.40 reduced coenzyme F420 + NADP+
-
 Methanoculleus thermophilus oxidized coenzyme F420 + NADPH + H+
-
 r
1.5.1.40 reduced coenzyme F420 + NADP+
-
 Methanofollis liminatans oxidized coenzyme F420 + NADPH + H+
-
 r
1.5.1.40 reduced coenzyme F420 + NADP+
-
 Methanobacterium palustre oxidized coenzyme F420 + NADPH + H+
-
 r
1.5.1.40 reduced coenzyme F420 + NADP+ the enzyme exhibits a sequential kinetic mechanism Methanogenium organophilum oxidized coenzyme F420 + NADPH + H+
-
 r
1.5.1.40 reduced coenzyme F420 + NADP+
-
 Methanofollis liminatans DSM 4140 oxidized coenzyme F420 + NADPH + H+
-
 r
1.5.1.40 reduced coenzyme F420 + NADP+ the enzyme exhibits a sequential kinetic mechanism Methanogenium organophilum DSM 3596 oxidized coenzyme F420 + NADPH + H+
-
 r
1.5.1.40 reduced coenzyme F420 + NADP+
-
 Methanobacterium palustre DSM 3108 oxidized coenzyme F420 + NADPH + H+
-
 r
1.5.1.40 reduced coenzyme F420 + NADP+
-
 Methanoculleus thermophilus DSM 3915 oxidized coenzyme F420 + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.5.1.40 ? x * 28000, SDS-PAGE Methanogenium organophilum

Synonyms

EC Number Synonyms Comment Organism
1.5.1.40 F420-dependent NADP reductase
-
 Methanoculleus thermophilus
1.5.1.40 F420-dependent NADP reductase
-
 Methanofollis liminatans
1.5.1.40 F420-dependent NADP reductase
-
 Methanobacterium palustre
1.5.1.40 F420-dependent NADP reductase
-
 Methanogenium organophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.40 34
-
-
 Methanogenium organophilum
1.5.1.40 37
-
 assay at Methanofollis liminatans
1.5.1.40 37
-
 assay at Methanobacterium palustre
1.5.1.40 55
-
 assay at Methanoculleus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.40 5.5
-
 pH optimum for reduction of oxidized coenzyme F420 with NADPH Methanogenium organophilum
1.5.1.40 9.2
-
 pH optimum for reduction of NADP+ with reduced coenzyme F420 Methanogenium organophilum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.40 7.5 10 pH 7.5: about 50% of maximal activity, pH 10.0: about 90% of maximal activity, reduction of NADP+ with reduced coenzyme F420 Methanogenium organophilum