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Literature summary extracted from

  • Fiala, K.A.; Suo, Z.
    Pre-steady-state kinetic studies of the fidelity of Sulfolobus solfataricus P2 DNA polymerase IV (2004), Biochemistry, 43, 2106-2115.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.7 Dpo4 fused to a C-terminal His6 tag is expressed in Escherichia coli strain BL21(DE3) Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.7 MgCl2 optimal concentration: 5 mM Saccharolobus solfataricus
2.7.7.7 NaCl optimal concentration: 0-75 mM Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Saccharolobus solfataricus Q97W02
-
-
2.7.7.7 Saccharolobus solfataricus P2 Q97W02
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.7
-
Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn the fidelity of Dpo4 is in the range of 0.001-0.0001. The ground-state binding affinity of correct nucleotides is 10-50-fold weaker than those of replicative DNA polymerases. The affinity of incorrect nucleotides for Dpo4 is about 2-10-fold weaker than that of correct nucleotides. The mismatched dCTP has an affinity similar to that of the matched nucleotides when it is incorporated against a pyrimidine template base flanked by a 5'-template guanine. The mismatch incorporation rates, regardless of the 5'-template base, are about 2-3 orders of magnitude slower than the incorporation rates for matched nucleotides, which is the predominant contribution to the fidelity of Dpo4 Saccharolobus solfataricus diphosphate + DNAn+1
-
?
2.7.7.7 deoxynucleoside triphosphate + DNAn the fidelity of Dpo4 is in the range of 0.001-0.0001. The ground-state binding affinity of correct nucleotides is 10-50-fold weaker than those of replicative DNA polymerases. The affinity of incorrect nucleotides for Dpo4 is about 2-10-fold weaker than that of correct nucleotides. The mismatched dCTP has an affinity similar to that of the matched nucleotides when it is incorporated against a pyrimidine template base flanked by a 5'-template guanine. The mismatch incorporation rates, regardless of the 5'-template base, are about 2-3 orders of magnitude slower than the incorporation rates for matched nucleotides, which is the predominant contribution to the fidelity of Dpo4 Saccharolobus solfataricus P2 diphosphate + DNAn+1
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.7 DNA polymerase IV
-
Saccharolobus solfataricus
2.7.7.7 Dpo4
-
Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.7 7.5
-
-
Saccharolobus solfataricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.7 7 9 pH 7.0: about 50% of maximal activity, pH 9.0: about 60% of maximal activity Saccharolobus solfataricus