EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.7.7 | Dpo4 fused to a C-terminal His6 tag is expressed in Escherichia coli strain BL21(DE3) | Saccharolobus solfataricus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.7 | MgCl2 | optimal concentration: 5 mM | Saccharolobus solfataricus | |
2.7.7.7 | NaCl | optimal concentration: 0-75 mM | Saccharolobus solfataricus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.7 | Saccharolobus solfataricus | Q97W02 | - |
- |
2.7.7.7 | Saccharolobus solfataricus P2 | Q97W02 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.7 | - |
Saccharolobus solfataricus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.7 | deoxynucleoside triphosphate + DNAn | the fidelity of Dpo4 is in the range of 0.001-0.0001. The ground-state binding affinity of correct nucleotides is 10-50-fold weaker than those of replicative DNA polymerases. The affinity of incorrect nucleotides for Dpo4 is about 2-10-fold weaker than that of correct nucleotides. The mismatched dCTP has an affinity similar to that of the matched nucleotides when it is incorporated against a pyrimidine template base flanked by a 5'-template guanine. The mismatch incorporation rates, regardless of the 5'-template base, are about 2-3 orders of magnitude slower than the incorporation rates for matched nucleotides, which is the predominant contribution to the fidelity of Dpo4 | Saccharolobus solfataricus | diphosphate + DNAn+1 | - |
? | |
2.7.7.7 | deoxynucleoside triphosphate + DNAn | the fidelity of Dpo4 is in the range of 0.001-0.0001. The ground-state binding affinity of correct nucleotides is 10-50-fold weaker than those of replicative DNA polymerases. The affinity of incorrect nucleotides for Dpo4 is about 2-10-fold weaker than that of correct nucleotides. The mismatched dCTP has an affinity similar to that of the matched nucleotides when it is incorporated against a pyrimidine template base flanked by a 5'-template guanine. The mismatch incorporation rates, regardless of the 5'-template base, are about 2-3 orders of magnitude slower than the incorporation rates for matched nucleotides, which is the predominant contribution to the fidelity of Dpo4 | Saccharolobus solfataricus P2 | diphosphate + DNAn+1 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.7.7 | DNA polymerase IV | - |
Saccharolobus solfataricus |
2.7.7.7 | Dpo4 | - |
Saccharolobus solfataricus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.7 | 7.5 | - |
- |
Saccharolobus solfataricus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.7 | 7 | 9 | pH 7.0: about 50% of maximal activity, pH 9.0: about 60% of maximal activity | Saccharolobus solfataricus |