EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.2.1.B26 | structures, at approximately 2 A resolution, of the enzyme in complex with both covalently (derived from 2-fluoro-glycosides) and noncovalently (hydroximolactam) bound inhibitors | Saccharolobus solfataricus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.B26 | D-galactohydroximolactam | - |
Saccharolobus solfataricus | |
3.2.1.B26 | D-glucohydroximolactam | - |
Saccharolobus solfataricus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.B26 | 0.16 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.5, 37°C | Saccharolobus solfataricus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.B26 | 56690 | - |
quadrupole time-of-flight electrospray mass spectrometry | Saccharolobus solfataricus |
3.2.1.B26 | 56692 | - |
x * 56692, calculated from sequence | Saccharolobus solfataricus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.B26 | Saccharolobus solfataricus | P22498 | - |
- |
3.2.1.B26 | Saccharolobus solfataricus P2 | P22498 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.B26 | - |
Saccharolobus solfataricus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.B26 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + D-glucopyranose | - |
? | |
3.2.1.B26 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Saccharolobus solfataricus P2 | 4-nitrophenol + D-glucopyranose | - |
? | |
3.2.1.B26 | additional information | the enzyme has broad specificity, accommodating both gluco- and galacto-configured substrates | Saccharolobus solfataricus | ? | - |
? | |
3.2.1.B26 | additional information | the enzyme has broad specificity, accommodating both gluco- and galacto-configured substrates | Saccharolobus solfataricus P2 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.B26 | ? | x * 56692, calculated from sequence | Saccharolobus solfataricus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.B26 | GH1 beta-glycosidase | - |
Saccharolobus solfataricus |
3.2.1.B26 | Ssbeta-Glc1 | - |
Saccharolobus solfataricus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.B26 | 37 | - |
assay at | Saccharolobus solfataricus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.B26 | 4.2 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.5, 37°C | Saccharolobus solfataricus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.B26 | 6.5 | - |
assay at | Saccharolobus solfataricus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.B26 | 0.001 | - |
D-glucohydroximolactam | pH 6.5, 37°C | Saccharolobus solfataricus | |
3.2.1.B26 | 0.0011 | - |
D-galactohydroximolactam | pH 6.5, 37°C | Saccharolobus solfataricus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.B26 | 26.25 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.5, 37°C | Saccharolobus solfataricus |