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Literature summary extracted from

  • Kuorelahti, S.; Kalkkinen, N.; Penttilä, M.; Londesborough, J.; Richard, P.
    Identification in the mold Hypocrea jecorina of the first fungal D-galacturonic acid reductase (2005), Biochemistry, 44, 11234-11240.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.365 expressed in Saccharomyces cerevisiae strain CEN.PK2-1B Trichoderma reesei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.365 0.001
-
 NADP+ in 100 mM sodium phosphate (pH 7.0), at 22°C Trichoderma reesei
1.1.1.365 0.03
-
 NADPH in 100 mM sodium phosphate (pH 7.0), at 22°C Trichoderma reesei
1.1.1.365 4
-
 L-galactonate in 100 mM sodium phosphate (pH 7.0), at 22°C Trichoderma reesei
1.1.1.365 6
-
 DL-glyceraldehyde in 100 mM sodium phosphate (pH 7.0), at 22°C Trichoderma reesei
1.1.1.365 6
-
 D-galacturonate in 100 mM sodium phosphate (pH 7.0), at 22°C Trichoderma reesei
1.1.1.365 11
-
 D-glucuronic acid in 100 mM sodium phosphate (pH 7.0), at 22°C Trichoderma reesei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.365 40000
-
 x * 40000, SDS-PAGE Trichoderma reesei

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.365 Trichoderma reesei
-
-
-
1.1.1.365 Trichoderma reesei RUT C-30
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.365 Fractogel TSK DEAE-650 column chromatography Trichoderma reesei

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.365 culture condition:D-galacturonate-grown cell
-
 Trichoderma reesei
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.365 D-galacturonate + NADPH + H+
-
 Trichoderma reesei L-galactonate + NADP+
-
 r
1.1.1.365 D-galacturonate + NADPH + H+
-
 Trichoderma reesei RUT C-30 L-galactonate + NADP+
-
 r
1.1.1.365 D-glucuronic acid + NADPH + H+
-
 Trichoderma reesei ?
-
 ?
1.1.1.365 D-glucuronic acid + NADPH + H+
-
 Trichoderma reesei RUT C-30 ?
-
 ?
1.1.1.365 DL-glyceraldehyde + NADPH + H+
-
 Trichoderma reesei ?
-
 ?
1.1.1.365 DL-glyceraldehyde + NADPH + H+
-
 Trichoderma reesei RUT C-30 ?
-
 ?
1.1.1.365 L-galactonate + NADP+
-
 Trichoderma reesei D-galacturonate + NADPH + H+
-
 r
1.1.1.365 L-galactonate + NADP+
-
 Trichoderma reesei RUT C-30 D-galacturonate + NADPH + H+
-
 r
1.1.1.365 additional information no activity with NADH and D-glucose, D-fructose, D-xylose, D-galactose, L-arabinose, or D-mannose. No backward reaction with L-gulonic acid, glycerol, D-arabitol, L-arabitol, xylitol, galactitol, or ribitol Trichoderma reesei ?
-
 ?
1.1.1.365 additional information no activity with NADH and D-glucose, D-fructose, D-xylose, D-galactose, L-arabinose, or D-mannose. No backward reaction with L-gulonic acid, glycerol, D-arabitol, L-arabitol, xylitol, galactitol, or ribitol Trichoderma reesei RUT C-30 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.365 ? x * 40000, SDS-PAGE Trichoderma reesei

Synonyms

EC Number Synonyms Comment Organism
1.1.1.365 D-galacturonic acid reductase
-
 Trichoderma reesei

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.365 NADPH dependent on Trichoderma reesei