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Literature summary extracted from

  • Alvey, R.M.; Biswas, A.; Schluchter, W.M.; Bryant, D.A.
    Attachment of noncognate chromophores to CpcA of Synechocystis sp. PCC 6803 and Synechococcus sp. PCC 7002 by heterologous expression in Escherichia coli (2011), Biochemistry, 50, 4890-4902.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.7.4 recombinant functional expression of HT-HY2 in Escherichia coli with production of phytochromobilin, functional co-expression with cyanobacterial heme oxygenase, and the phycocyanin alpha-subunit, CpcA, from Synechocystis sp. PCC 6803 or Synechococcus sp. PCC 7002, and with the phycocyanin alpha-subunit phycocyanobilin lyase, CpcE/CpcF, or the phycoerythrocyanin alpha-subunit phycocyanobilin isomerizing lyase, PecE/PecF, from Noctoc sp. PCC 7120. Production levels of fluorescent pigments and chromophore analysis, overview Arabidopsis thaliana
1.3.7.5 recombinant functional expression of PcyA in Escherichia coli with production of phycocyanobilin, functional co-expression with cyanobacterial heme oxygenase, and the phycocyanin alpha-subunit, CpcA, from Synechocystis sp. PCC 6803 or Synechococcus sp. PCC 7002, and with the phycocyanin alpha-subunit phycocyanobilin lyase, CpcE/CpcF, or the phycoerythrocyanin alpha-subunit phycocyanobilin isomerizing lyase, PecE/PecF, from Noctoc sp. PCC 7120. Production levels of fluorescent pigments and chromophore analysis, overview Synechococcus sp.
1.3.7.6 gene pebS, production of His-tagged phycocyanin alpha-subunit-(3Z)-phycoerythrobilin using the alternative FDBR, phycoerythrobilin synthase, PebS, overview. Recombinant functional expression of PebS in Escherichia coli, co-expression with phycocyanin alpha-subunit, CpcA, from Synechocystis sp. PCC 6803 or Synechococcus sp. PCC 7002, cyanobacterial heme oxygenase, and with the phycocyanin alpha-subunit phycocyanobilin lyase, CpcE/CpcF, or the phycoerythrocyanin alpha-subunit phycocyanobilin isomerizing lyase, PecE/PecF, from Noctoc sp. PCC 7120. Production levels of fluorescent pigments and chromophore analysis, overview Prochlorococcus phage P-SSM4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.7.4 (3Z)-phytochromobilin + oxidized ferredoxin Arabidopsis thaliana
-
 biliverdin IXalpha + reduced ferredoxin
-
 ?
1.3.7.5 biliverdin Ixalpha + reduced ferredoxin Synechococcus sp.
-
 (3Z)-phycocyanobilin + oxidized ferredoxin
-
 ?
1.3.7.5 biliverdin Ixalpha + reduced ferredoxin Synechococcus sp. 7002
-
 (3Z)-phycocyanobilin + oxidized ferredoxin
-
 ?
1.3.7.6 biliverdin IXalpha + 2 reduced ferredoxin Prochlorococcus phage P-SSM4
-
 (3Z)-phycoerythrobilin + 2 oxidized ferredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.7.4 Arabidopsis thaliana
-
-
-
1.3.7.5 Synechococcus sp.
-
-
-
1.3.7.5 Synechococcus sp. 7002
-
-
-
1.3.7.6 Prochlorococcus phage P-SSM4
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.7.4 (3Z)-phytochromobilin + oxidized ferredoxin
-
 Arabidopsis thaliana biliverdin IXalpha + reduced ferredoxin
-
 ?
1.3.7.5 biliverdin Ixalpha + reduced ferredoxin
-
 Synechococcus sp. (3Z)-phycocyanobilin + oxidized ferredoxin
-
 ?
1.3.7.5 biliverdin Ixalpha + reduced ferredoxin
-
 Synechococcus sp. 7002 (3Z)-phycocyanobilin + oxidized ferredoxin
-
 ?
1.3.7.6 biliverdin IXalpha + 2 reduced ferredoxin
-
 Prochlorococcus phage P-SSM4 (3Z)-phycoerythrobilin + 2 oxidized ferredoxin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.7.4 3Z-phytochromobilin:ferredoxin oxidoreductase
-
 Arabidopsis thaliana
1.3.7.4 HT-HY2
-
 Arabidopsis thaliana
1.3.7.4 HY2
-
 Arabidopsis thaliana
1.3.7.5 3Z-phycocyanobilin:ferredoxin oxidoreductase
-
 Synechococcus sp.
1.3.7.5 PcyA
-
 Synechococcus sp.
1.3.7.6 PebS
-
 Prochlorococcus phage P-SSM4
1.3.7.6 phycoerythrobilin synthase
-
 Prochlorococcus phage P-SSM4

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.7.4 Ferredoxin
-
 Arabidopsis thaliana
1.3.7.5 Ferredoxin
-
 Synechococcus sp.
1.3.7.6 Ferredoxin
-
 Prochlorococcus phage P-SSM4

General Information

EC Number General Information Comment Organism
1.3.7.4 physiological function plants utilize 3Z-phytochromobilin:ferredoxin oxidoreductase to reduce the diene system of the A ring of biliverdin IXalpha to form PthetaB Arabidopsis thaliana
1.3.7.5 additional information cyanobacteria utilize phycocyanobilin:ferredoxin oxidoreductase (PcyA) to perform a two-step reaction, the enzyme first reduces the 18-vinyl side chain of the D-ring and subsequently reduces the vinyl side chain of the pyrrole A ring to yield phycocyanobilin Synechococcus sp.