Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Luo, M.; Arentson, B.W.; Srivastava, D.; Becker, D.F.; Tanner, J.J.
    Crystal structures and kinetics of monofunctional proline dehydrogenase provide insight into substrate recognition and conformational changes associated with flavin reduction and product release (2012), Biochemistry, 51, 10099-10108.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.99.B2 expressed in Escherichia coli BL21(DE3)pLysS cells Deinococcus radiodurans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.5.99.B2 enzyme in the oxidized state complexed with the proline analogue L-tetrahydrofuroic acid and in the reduced state with the proline site vacant, sitting drop vapor diffusion method, using 0.2 M MgCl2, 25% (w/v) PEG 3350, and 0.1 mM Bis-Tris (pH 5.8), at 22°C Deinococcus radiodurans

Protein Variants

EC Number Protein Variants Comment Organism
1.5.99.B2 E64A the mutation decreases the catalytic efficiency 27fold Deinococcus radiodurans
1.5.99.B2 G63A the mutation decreases the catalytic efficiency 140fold Deinococcus radiodurans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.99.B2 0.028
-
coenzyme Q1 mutant enzyme G63A, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) Deinococcus radiodurans
1.5.99.B2 0.032
-
coenzyme Q1 mutant enzyme E64A, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) Deinococcus radiodurans
1.5.99.B2 0.155
-
coenzyme Q1 wild type enzyme, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) Deinococcus radiodurans
1.5.99.B2 50
-
L-proline mutant enzyme E64A, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) Deinococcus radiodurans
1.5.99.B2 290
-
L-proline wild type enzyme, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) Deinococcus radiodurans
1.5.99.B2 384
-
L-proline mutant enzyme G63A, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) Deinococcus radiodurans

Organism

EC Number Organism UniProt Comment Textmining
1.5.99.B2 Deinococcus radiodurans Q9RW55
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.99.B2 HisTrap column chromatography and Hitrap Q column chromatography Deinococcus radiodurans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.99.B2 L-proline + coenzyme Q1 + H2O
-
Deinococcus radiodurans DELTA1-pyrroline-5-carboxylate + reduced coenzyme Q1
-
?

Synonyms

EC Number Synonyms Comment Organism
1.5.99.B2 PRODH
-
Deinococcus radiodurans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.99.B2 0.043
-
coenzyme Q1 mutant enzyme G63A, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) Deinococcus radiodurans
1.5.99.B2 0.046
-
coenzyme Q1 mutant enzyme E64A, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) Deinococcus radiodurans
1.5.99.B2 0.055
-
L-proline mutant enzyme E64A, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) Deinococcus radiodurans
1.5.99.B2 0.08
-
L-proline mutant enzyme G63A, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) Deinococcus radiodurans
1.5.99.B2 8.7
-
L-proline wild type enzyme, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) Deinococcus radiodurans
1.5.99.B2 14
-
coenzyme Q1 wild type enzyme, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) Deinococcus radiodurans

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.99.B2 FAD dependent on Deinococcus radiodurans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.5.5.2 0.00021
-
L-proline mutant enzyme G63A, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) Deinococcus radiodurans
1.5.5.2 0.0011
-
L-proline mutant enzyme E64A, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) Deinococcus radiodurans
1.5.5.2 0.03
-
L-proline wild type enzyme, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) Deinococcus radiodurans
1.5.5.2 1.438
-
coenzyme Q1 mutant enzyme E64A, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) Deinococcus radiodurans
1.5.5.2 1.535
-
coenzyme Q1 mutant enzyme G63A, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) Deinococcus radiodurans
1.5.5.2 90.32
-
coenzyme Q1 wild type enzyme, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) Deinococcus radiodurans