EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.99.B2 | expressed in Escherichia coli BL21(DE3)pLysS cells | Deinococcus radiodurans |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.5.99.B2 | enzyme in the oxidized state complexed with the proline analogue L-tetrahydrofuroic acid and in the reduced state with the proline site vacant, sitting drop vapor diffusion method, using 0.2 M MgCl2, 25% (w/v) PEG 3350, and 0.1 mM Bis-Tris (pH 5.8), at 22°C | Deinococcus radiodurans |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.5.99.B2 | E64A | the mutation decreases the catalytic efficiency 27fold | Deinococcus radiodurans |
1.5.99.B2 | G63A | the mutation decreases the catalytic efficiency 140fold | Deinococcus radiodurans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.99.B2 | 0.028 | - |
coenzyme Q1 | mutant enzyme G63A, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) | Deinococcus radiodurans | |
1.5.99.B2 | 0.032 | - |
coenzyme Q1 | mutant enzyme E64A, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) | Deinococcus radiodurans | |
1.5.99.B2 | 0.155 | - |
coenzyme Q1 | wild type enzyme, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) | Deinococcus radiodurans | |
1.5.99.B2 | 50 | - |
L-proline | mutant enzyme E64A, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) | Deinococcus radiodurans | |
1.5.99.B2 | 290 | - |
L-proline | wild type enzyme, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) | Deinococcus radiodurans | |
1.5.99.B2 | 384 | - |
L-proline | mutant enzyme G63A, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) | Deinococcus radiodurans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.99.B2 | Deinococcus radiodurans | Q9RW55 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.99.B2 | HisTrap column chromatography and Hitrap Q column chromatography | Deinococcus radiodurans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.99.B2 | L-proline + coenzyme Q1 + H2O | - |
Deinococcus radiodurans | DELTA1-pyrroline-5-carboxylate + reduced coenzyme Q1 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.99.B2 | PRODH | - |
Deinococcus radiodurans |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.99.B2 | 0.043 | - |
coenzyme Q1 | mutant enzyme G63A, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) | Deinococcus radiodurans | |
1.5.99.B2 | 0.046 | - |
coenzyme Q1 | mutant enzyme E64A, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) | Deinococcus radiodurans | |
1.5.99.B2 | 0.055 | - |
L-proline | mutant enzyme E64A, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) | Deinococcus radiodurans | |
1.5.99.B2 | 0.08 | - |
L-proline | mutant enzyme G63A, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) | Deinococcus radiodurans | |
1.5.99.B2 | 8.7 | - |
L-proline | wild type enzyme, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) | Deinococcus radiodurans | |
1.5.99.B2 | 14 | - |
coenzyme Q1 | wild type enzyme, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) | Deinococcus radiodurans |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.99.B2 | FAD | dependent on | Deinococcus radiodurans |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.5.2 | 0.00021 | - |
L-proline | mutant enzyme G63A, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) | Deinococcus radiodurans | |
1.5.5.2 | 0.0011 | - |
L-proline | mutant enzyme E64A, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) | Deinococcus radiodurans | |
1.5.5.2 | 0.03 | - |
L-proline | wild type enzyme, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) | Deinococcus radiodurans | |
1.5.5.2 | 1.438 | - |
coenzyme Q1 | mutant enzyme E64A, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) | Deinococcus radiodurans | |
1.5.5.2 | 1.535 | - |
coenzyme Q1 | mutant enzyme G63A, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) | Deinococcus radiodurans | |
1.5.5.2 | 90.32 | - |
coenzyme Q1 | wild type enzyme, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5) | Deinococcus radiodurans |