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Literature summary extracted from
- Human sulfide:Quinone oxidoreductase catalyzes the first step in hydrogen sulfide metabolism and produces a sulfane sulfur metabolite (2012), Biochemistry, 51, 6804-6815.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.5.4 | expression at low temperature in Escherichia coli by using an optimized synthetic gene and cold-adapted chaperonins | Homo sapiens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.5.4 | 0.0109 | - |
Sulfide | cosubstrates cyanide, ubiquinone-1, pH 8.5, 25°C | Homo sapiens |  |
| 1.8.5.4 | 0.013 | - |
Sulfide | cosubstrates sulfite, ubiquinone-1, pH 7.5, 25°C | Homo sapiens | |
| 1.8.5.4 | 0.014 | - |
ubiquinone-1 | cosubstrates cyanide, sulfide, pH 8.5, 25°C | Homo sapiens | |
| 1.8.5.4 | 0.0199 | - |
ubiquinone-1 | cosubstrates sulfite, sulfide, pH 7.5, 25°C | Homo sapiens | |
| 1.8.5.4 | 0.174 | - |
sulfite | cosubstrates sulfide, ubiquinone-1, pH 7.5, 25°C | Homo sapiens | |
| 1.8.5.4 | 0.315 | - |
Sulfide | cosubstrates sulfide, ubiquinone-1, pH 7.0, 25°C | Homo sapiens | |
| 1.8.5.4 | 0.65 | - |
cyanide | cosubstrates sulfide, ubiquinone-1, pH 8.5, 25°C | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.5.4 | additional information | Homo sapiens | cyanide, sulfite, or sulfide can act as the sulfane sulfur acceptor in reactions that exhibit pH optima at 8.5, 7.5, or 7.0, respectively, and produce thiocyanate, thiosulfate, or a putative sulfur analogue of hydrogen peroxide, i.e. H2S2, respectively. Sulfite is the physiological acceptor of the sulfur and the reaction is the predominant source of the thiosulfate produced during H2S oxidation by mammalian tissues | ? | - |
? | |
| 1.8.5.4 | sulfide + ubiquinone-1 | Homo sapiens | - |
sulfur + ubiquinol-1 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.5.4 | Homo sapiens | Q9Y6N5 | - |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.5.4 | 581 | - |
pH 7.5, 25°C | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.5.4 | 2 sulfide + ubiquinone-1 | - |
Homo sapiens | hydrogen disulfide + ubiquinol-1 | - |
? | |
| 1.8.5.4 | additional information | cyanide, sulfite, or sulfide can act as the sulfane sulfur acceptor in reactions that exhibit pH optima at 8.5, 7.5, or 7.0, respectively, and produce thiocyanate, thiosulfate, or a putative sulfur analogue of hydrogen peroxide, i.e. H2S2, respectively. Sulfite is the physiological acceptor of the sulfur and the reaction is the predominant source of the thiosulfate produced during H2S oxidation by mammalian tissues | Homo sapiens | ? | - |
? | |
| 1.8.5.4 | sulfide + cyanide + ubiquinone-1 | - |
Homo sapiens | thiocyanate + ubiquinol-1 | - |
? | |
| 1.8.5.4 | sulfide + sulfite + ubiquinone-1 | - |
Homo sapiens | thiosulfate + ubiquinol-1 | - |
? | |
| 1.8.5.4 | sulfide + ubiquinone-1 | - |
Homo sapiens | sulfur + ubiquinol-1 | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.5.4 | 65 | - |
Sulfide | cosubstrates sulfide, ubiquinone-1, pH 7.0, 25°C | Homo sapiens | |
| 1.8.5.4 | 330 | - |
cyanide | cosubstrates sulfide, ubiquinone-1, pH 8.5, 25°C | Homo sapiens | |
| 1.8.5.4 | 343 | - |
Sulfide | cosubstrates cyanide, ubiquinone-1, pH 8.5, 25°C | Homo sapiens | |
| 1.8.5.4 | 360 | - |
ubiquinone-1 | cosubstrates cyanide, sulfide, pH 8.5, 25°C | Homo sapiens | |
| 1.8.5.4 | 364 | - |
ubiquinone-1 | cosubstrates sulfite, sulfide, pH 7.5, 25°C | Homo sapiens | |
| 1.8.5.4 | 368 | - |
sulfite | cosubstrates sulfide, ubiquinone-1, pH 7.5, 25°C | Homo sapiens | |
| 1.8.5.4 | 379 | - |
Sulfide | cosubstrates sulfite, ubiquinone-1, pH 7.5, 25°C | Homo sapiens | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.5.4 | FAD | protein contains noncovalently bound FAD | Homo sapiens | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.5.4 | 2100 | - |
Sulfide | cosubstrates sulfide, ubiquinone-1, pH 7.0, 25°C | Homo sapiens | |
| 1.8.5.4 | 2100 | - |
sulfite | cosubstrates sulfide, ubiquinone-1, pH 7.5, 25°C | Homo sapiens | |
| 1.8.5.4 | 5100 | - |
cyanide | cosubstrates sulfide, ubiquinone-1, pH 8.5, 25°C | Homo sapiens | |
| 1.8.5.4 | 19000 | - |
ubiquinone-1 | cosubstrates sulfite, sulfide, pH 7.5, 25°C | Homo sapiens | |
| 1.8.5.4 | 27000 | - |
ubiquinone-1 | cosubstrates cyanide, sulfide, pH 8.5, 25°C | Homo sapiens | |
| 1.8.5.4 | 29000 | - |
Sulfide | cosubstrates sulfite, ubiquinone-1, pH 7.5, 25°C | Homo sapiens | |
| 1.8.5.4 | 31000 | - |
Sulfide | cosubstrates cyanide, ubiquinone-1, pH 8.5, 25°C | Homo sapiens | |
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