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Literature summary extracted from

  • Kovaleva, E.G.; Lipscomb, J.D.
    Structural basis for the role of tyrosine 257 of homoprotocatechuate 2,3-dioxygenase in substrate and oxygen activation (2012), Biochemistry, 51, 8755-8763.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.15 crystallizations of wild-type FeHPCD and mutant Y257F with bound substrates 3,4-dihydroxyphenylacetate and 4-nitrocatechol, hanging drop vapour diffusion method, in either 13% PEG 6000, 0.1 M calcium chloride, 0.1 M Tris-HCl, pH 6.5-7.4 or in 18% PEG 8000, 0.1 M calcium acetate, 0.1 M sodium cacodylate, pH 6.5, at 20°C, cryoprotectant solution containing either 20-25% PEG400 or 20% glycerol in the mother liquor solution, X-ray diffraction structure determination and analysis at 1.50-1.60 A resolution Brevibacterium fuscum

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.15 Y257F site-directed mutagenesis, changes in kinetics for Y257F variant can be attributed to the loss of interactions between substrate and Tyr257, substrate binding structures compared to the wild-type enzyme, overview Brevibacterium fuscum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.15 Fe2+ enzyme-bound, required for catalysis Brevibacterium fuscum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.15 3,4-dihydroxyphenylacetate + O2 Brevibacterium fuscum
-
2-hydroxy-5-carboxymethylmuconate semialdehyde
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.15 Brevibacterium fuscum Q45135
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.15 3,4-dihydroxyphenylacetate + O2
-
Brevibacterium fuscum 2-hydroxy-5-carboxymethylmuconate semialdehyde
-
?
1.13.11.15 4-nitrocatechol + O2
-
Brevibacterium fuscum ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.15 FeHPCD
-
Brevibacterium fuscum
1.13.11.15 homoprotocatechuate 2,3-dioxygenase
-
Brevibacterium fuscum