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Literature summary extracted from

  • Yan, Z.; Fushinobu, S.; Wakagi, T.
    Four Cys residues in heterodimeric 2-oxoacid:ferredoxin oxidoreductase are required for CoA-dependent oxidative decarboxylation but not for a non-oxidative decarboxylation (2014), Biochim. Biophys. Acta, 1844, 736-743.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.2.7.11 C12/15A loss of ironĀ–sulfur cluster Sulfurisphaera tokodaii
1.2.7.11 C12A loss of ironĀ–sulfur cluster. The mutant enzyme does not show formation of any radical intermediate or production of acetyl-CoA Sulfurisphaera tokodaii
1.2.7.11 C15A loss of ironĀ–sulfur cluster. The mutant enzyme does not show formation of any radical intermediate or production of acetyl-CoA Sulfurisphaera tokodaii
1.2.7.11 C197A the enzyme retains an unidentified type of ironĀ–sulfur cluster Sulfurisphaera tokodaii
1.2.7.11 C46A loss of ironĀ–sulfur cluster. The mutant enzyme does not show formation of any radical intermediate or production of acetyl-CoA Sulfurisphaera tokodaii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.11 4Fe-4S center the enzyme has one [4FeĀ–4S]2+ cluster, ligated by 4 Cys residues, C12, C15, C46, and C197. All four Cys are required to fold a [4FeĀ–4S] cluster for oxidative decarboxylation of pyruvate including the formation of a stable hydroxyethyl-thiamine diphosphate radical Sulfurisphaera tokodaii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.7.11 pyruvate + CoA + 2 oxidized ferredoxin Sulfurisphaera tokodaii
-
 acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.11 Sulfurisphaera tokodaii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.11 wild-type and mutant enzymes Sulfurisphaera tokodaii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.11 pyruvate + CoA + 2 oxidized ferredoxin
-
 Sulfurisphaera tokodaii acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.7.11 heterodimer
-
 Sulfurisphaera tokodaii

Synonyms

EC Number Synonyms Comment Organism
1.2.7.11 2-oxoacid:ferredoxin oxidoreductase
-
 Sulfurisphaera tokodaii
1.2.7.11 OFOR
-
 Sulfurisphaera tokodaii

Temperature Optimum [Ā°C]

EC Number Temperature Optimum [Ā°C] Temperature Optimum Maximum [Ā°C] Comment Organism
1.2.7.11 80
-
 assay at Sulfurisphaera tokodaii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.11 8.5
-
 assay at Sulfurisphaera tokodaii