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Literature summary extracted from

  • Limauro, D.; D'Ambrosio, K.; Langella, E.; de Simone, G.; Galdi, I.; Pedone, C.; Pedone, E.; Bartolucci, S.
    Exploring the catalytic mechanism of the first dimeric Bcp: Functional, structural and docking analyses of Bcp4 from Sulfolobus solfataricus (2010), Biochimie, 92, 1435-1444.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.11.1.24 hanging-drop vapor-diffusion method, C45S/C50S double mutant crystallizes in the space group P2(1) with four molecules per asymmetric unit Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.24 C45S inactive in assay with H2O2 and reduced dithiothreitol Saccharolobus solfataricus
1.11.1.24 C50S still active in assay with H2O2 and reduced dithiothreitol. Mutant enzyme occurs in a dimeric and a tetrameric form Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.24 Saccharolobus solfataricus Q97VL0
-
-
1.11.1.24 Saccharolobus solfataricus P2 Q97VL0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.24
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.24 H2O2 + reduced dithiothreitol
-
 Saccharolobus solfataricus H2O + oxidized dithiothreitol
-
 ?
1.11.1.24 H2O2 + reduced dithiothreitol
-
 Saccharolobus solfataricus P2 H2O + oxidized dithiothreitol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.24 dimer
-
 Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
1.11.1.24 Bcp4
-
 Saccharolobus solfataricus
1.11.1.24 SSO2613 locus name Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.24 80
-
 assay at Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.24 7
-
 assay at Saccharolobus solfataricus