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Literature summary extracted from

  • Huang, F.C.; Schwab, W.
    Cloning and characterization of a 9-lipoxygenase gene induced by pathogen attack from Nicotiana benthamiana for biotechnological application (2011), BMC Biotechnol., 11, 30.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.13.11.58 synthesis the yeast expressed Nb-9-LOX can be used to produce C9-aldehydes on a large scale in combination with a HPL gene with 9-hydroperoxide lyase function, or to effectively produce 9-hydroxy-10(E),12(Z)-octadecadienoic acid in a biocatalytic process in combination with cysteine as a mild reducing agent Nicotiana benthamiana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.58 gene Nb-9-LOX, DNA and amino acid sequence determination and analysis, overexpression in Saccharomyces cerevisiae, quantitative realtime PCR exxpression analysis Nicotiana benthamiana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.58 0.0039
-
 linoleate pH 6.0, 35°C, recombinant enzyme Nicotiana benthamiana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.58 97400
-
 x * 97400, about, sequence calculation Nicotiana benthamiana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.58 linoleate + O2 Nicotiana benthamiana
-
 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
-
 ?
1.13.11.58 additional information Nicotiana benthamiana Nb-9-LOX possesses both 9-lipoxygenase and 13-lipoxygenase, EC 1.13.11.12, specificity, with high predominance for the 9-LOX function ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.58 Nicotiana benthamiana
-
 gene Nb-9-LOX
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.58 leaf
-
 Nicotiana benthamiana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.58 linoleate + O2
-
 Nicotiana benthamiana (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
-
 ?
1.13.11.58 linoleate + O2 regiospecificity of Nb-9-LOX, overview Nicotiana benthamiana (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
-
 ?
1.13.11.58 additional information Nb-9-LOX possesses both 9-lipoxygenase and 13-lipoxygenase, EC 1.13.11.12, specificity, with high predominance for the 9-LOX function Nicotiana benthamiana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.58 ? x * 97400, about, sequence calculation Nicotiana benthamiana

Synonyms

EC Number Synonyms Comment Organism
1.13.11.58 9-LOX
-
 Nicotiana benthamiana
1.13.11.58 Nb-9-LOX
-
 Nicotiana benthamiana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.58 35
-
-
 Nicotiana benthamiana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.58 6
-
-
 Nicotiana benthamiana

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.13.11.58 Nicotiana benthamiana sequence calculation
-
 5.52

Expression

EC Number Organism Comment Expression
1.13.11.58 Nicotiana benthamiana the enzyme expression is highly induced by agroinfiltration of the plant leaves using a tobacco mosaic virus based vector system up