EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.6.2.4 | D327A | about 75% of wild-type helicase activity | Saccharolobus solfataricus |
5.6.2.4 | E326A | about 95% of wild-type helicase activity | Saccharolobus solfataricus |
5.6.2.4 | E326A/D327A | about 85% of wild-type helicase activity | Saccharolobus solfataricus |
5.6.2.4 | K323A | about 40% of wild-type helicase activity | Saccharolobus solfataricus |
5.6.2.4 | K346A | no ATPase activity, no helicase activity | Saccharolobus solfataricus |
5.6.2.4 | L325A | about 40% of wild-type helicase activity | Saccharolobus solfataricus |
5.6.2.4 | R329A | about 50% of wild-type helicase activity | Saccharolobus solfataricus |
5.6.2.4 | R331A | no ATPase activity, no helicase activity | Saccharolobus solfataricus |
5.6.2.4 | V324A | about 90% of wild-type helicase activity | Saccharolobus solfataricus |
5.6.2.4 | V324A/L325A | about 80% of wild-type helicase activity | Saccharolobus solfataricus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.6.2.4 | 0.00006 | - |
ATP | pH and temperature not specified in the publication, mutant enzyme E326A | Saccharolobus solfataricus | |
5.6.2.4 | 0.00009 | - |
ATP | pH and temperature not specified in the publication, mutant enzyme E326A/D327A | Saccharolobus solfataricus | |
5.6.2.4 | 0.0001 | - |
ATP | pH and temperature not specified in the publication, mutant enzyme D327A | Saccharolobus solfataricus | |
5.6.2.4 | 0.00012 | - |
ATP | pH and temperature not specified in the publication, mutant enzyme V324A | Saccharolobus solfataricus | |
5.6.2.4 | 0.00014 | - |
ATP | pH and temperature not specified in the publication, mutant enzyme K323A | Saccharolobus solfataricus | |
5.6.2.4 | 0.00017 | - |
ATP | pH and temperature not specified in the publication, wild-type enzyme | Saccharolobus solfataricus | |
5.6.2.4 | 0.00017 | - |
ATP | pH and temperature not specified in the publication, mutant enzyme R329A | Saccharolobus solfataricus | |
5.6.2.4 | 0.00023 | - |
ATP | pH and temperature not specified in the publication, mutant enzyme V324A/L325A | Saccharolobus solfataricus | |
5.6.2.4 | 0.00027 | - |
ATP | pH and temperature not specified in the publication, mutant enzyme L325A | Saccharolobus solfataricus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.6.2.4 | Saccharolobus solfataricus | Q9UXG1 | - |
- |
5.6.2.4 | Saccharolobus solfataricus P2 | Q9UXG1 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.6.2.4 | - |
Saccharolobus solfataricus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.6.2.4 | ATP + H2O | the protein exterior hairpin reveals critical residues for helicase activity and DNA binding | Saccharolobus solfataricus | ADP + phosphate | - |
? | |
5.6.2.4 | ATP + H2O | the protein exterior hairpin reveals critical residues for helicase activity and DNA binding | Saccharolobus solfataricus P2 | ADP + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.6.2.4 | hexamer | - |
Saccharolobus solfataricus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.6.2.4 | mini-chromosome maintenance protein | - |
Saccharolobus solfataricus |
5.6.2.4 | SsoMCM | - |
Saccharolobus solfataricus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.6.2.4 | 0.03 | - |
ATP | pH and temperature not specified in the publication, mutant enzyme K323A | Saccharolobus solfataricus | |
5.6.2.4 | 0.03 | - |
ATP | pH and temperature not specified in the publication, mutant enzyme R329A | Saccharolobus solfataricus | |
5.6.2.4 | 0.033 | - |
ATP | pH and temperature not specified in the publication, mutant enzyme E326A/D327A | Saccharolobus solfataricus | |
5.6.2.4 | 0.038 | - |
ATP | pH and temperature not specified in the publication, mutant enzyme D327A | Saccharolobus solfataricus | |
5.6.2.4 | 0.04 | - |
ATP | pH and temperature not specified in the publication, mutant enzyme V324A | Saccharolobus solfataricus | |
5.6.2.4 | 0.048 | - |
ATP | pH and temperature not specified in the publication, mutant enzyme E326A | Saccharolobus solfataricus | |
5.6.2.4 | 0.065 | - |
ATP | pH and temperature not specified in the publication, mutant enzyme V324A/L325A | Saccharolobus solfataricus | |
5.6.2.4 | 0.067 | - |
ATP | pH and temperature not specified in the publication, wild-type enzyme | Saccharolobus solfataricus | |
5.6.2.4 | 0.08 | - |
ATP | pH and temperature not specified in the publication, mutant enzyme L325A | Saccharolobus solfataricus |