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Literature summary extracted from

  • Hancock, S.M.; Corbett, K.; Fordham-Skelton, A.P.; Gatehouse, J.A.; Davis, B.G.
    Developing promiscuous glycosidases for glycoside synthesis: residues W433 and E432 in Sulfolobus solfataricus beta-glycosidase are important glucoside- and galactoside-specificity determinants (2005), Chembiochem, 6, 866-875.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.B26 synthesis formation of mannosides and xylosides by transglycosylation Saccharolobus solfataricus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.B26 wild-type and mutated sequences are expressed in Escherichia coli with a His7-tag to allow one-step chromatographic purification Saccharolobus solfataricus
3.2.1.B26 wild-type enzyme and mutant enzymes E432C and W433C are expressed in Escherichia coli with a His7-tag to allow one-step chromatographic purification Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.B26 E432C kcat/KM for 4-methylumbelliferyl beta-D-glucoside is reduced 200fold, kcat/KM for 4-methylumbelliferyl beta-D-galactoside is reduced 130fold, kcat/KM for 4-methylumbelliferyl beta-D-xyloside is reduced 1.5fold, kcat/KM for 4-methylumbelliferyl beta-D-fucoside is reduced 2900fold, kcat/KM for 4-methylumbelliferyl beta-D-mannoside is ibcreased 1.5fold Saccharolobus solfataricus
3.2.1.B26 E432C the kcat/KM for 4-methylumbelliferyl beta-D-glucoside is reduced 200fold, and 4-methylumbelliferyl beta-D-galactoside is reduced 130fold. Although the activities with 4-methylumbelliferyl beta-D-mannoside and 4-methylumbelliferyl beta-D-xyloside are also reduced in the mutant, this is to a much smaller extent. The mutant enzyme shows remarkably broader specificity than wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 W433C kcat/KM for 4-methylumbelliferyl beta-D-glucoside is reduced 140fold, kcat/KM for 4-methylumbelliferyl beta-D-galactoside is reduced 230fold, kcat/KM for 4-methylumbelliferyl beta-D-xyloside is reduced 14fold, kcat/KM for 4-methylumbelliferyl beta-D-fucoside is reduced 138fold, kcat/KM for 4-methylumbelliferyl beta-D-mannoside is reduced 16fold Saccharolobus solfataricus
3.2.1.B26 W433C the kcat/KM for 4-methylumbelliferyl beta-D-glucoside is reduced 140fold, and 4-methylumbelliferyl beta-D-galactoside is reduced 230fold. Although the activities with 4-methylumbelliferyl beta-D-mannoside and 4-methylumbelliferyl beta-D-xyloside are also reduced in the mutant, this is to a much smaller extent. The mutant enzyme shows remarkably broader specificity than wild-type enzyme Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.B26 additional information the activities of the wild-type enzyme in 30% acetonitrile, butanone and acetone are higher than those in dimethyl sulfoxide, dimethylformamide and 1,4-dioxane. The low boiling point of acetone limits the application of this solvent in thermophilic enzyme reactions Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.B26 0.011
-
 4-methylumbelliferyl beta-D-fucoside pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.011
-
 4-methylumbelliferyl beta-D-fucoside pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.036
-
 4-methylumbelliferyl beta-D-mannoside pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.036
-
 4-methylumbelliferyl beta-D-mannoside pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.046
-
 4-methylumbelliferyl beta-D-glucoside pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.046
-
 4-methylumbelliferyl beta-D-glucoside pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.066
-
 4-methylumbelliferyl beta-D-galactoside pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.066
-
 4-methylumbelliferyl beta-D-galactoside pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.13
-
 4-methylumbelliferyl beta-D-xyloside pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.13
-
 4-methylumbelliferyl beta-D-xyloside pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.15
-
 4-nitrophenyl beta-D-glucoside pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.15
-
 4-methylumbelliferyl beta-D-glucoside pH 6.5, 45°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.18
-
 4-methylumbelliferyl beta-D-mannoside pH 6.5, 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 0.21
-
 4-nitrophenyl beta-D-fucoside pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.21
-
 4-methylumbelliferyl beta-D-fucoside pH 6.5, 45°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.34
-
 4-methylumbelliferyl beta-D-glucoside pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.34
-
 4-methylumbelliferyl beta-D-fucoside pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.34
-
 4-methylumbelliferyl beta-D-glucoside pH 6.5, 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.34
-
 4-methylumbelliferyl beta-D-fucoside pH 6.5, 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.41
-
 4-methylumbelliferyl beta-D-fucoside pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 0.41
-
 4-methylumbelliferyl beta-D-fucoside pH 6.5, 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 0.47
-
 4-methylumbelliferyl beta-D-galactoside pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.47
-
 4-methylumbelliferyl beta-D-galactoside pH 6.5, 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.51
-
 4-nitrophenyl beta-D-glucoside pH 6.5 (50 mM phosphate), 45°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.51
-
 4-methylumbelliferyl beta-D-glucoside pH 6.5, 45°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.57
-
 4-nitrophenyl beta-D-galactoside pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.57
-
 4-methylumbelliferyl beta-D-galactoside pH 6.5, 45°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.59
-
 4-methylumbelliferyl beta-D-xyloside pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 0.59
-
 4-methylumbelliferyl beta-D-xyloside pH 6.5, 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 0.81
-
 4-nitrophenyl beta-D-galactoside pH 6.5 (50 mM phosphate), 45°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.81
-
 4-methylumbelliferyl beta-D-galactoside pH 6.5, 45°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.9
-
 4-methylumbelliferyl beta-D-mannoside pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.9
-
 4-methylumbelliferyl beta-D-mannoside pH 6.5, 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 1.26
-
 4-methylumbelliferyl beta-D-xyloside pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 1.26
-
 4-methylumbelliferyl beta-D-xyloside pH 6.5, 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 1.3
-
 4-methylumbelliferyl beta-D-glucuronide pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 1.3
-
 4-methylumbelliferyl beta-D-galacturonate pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 1.61
-
 4-methylumbelliferyl beta-D-glucoside pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 1.61
-
 4-methylumbelliferyl beta-D-glucoside pH 6.5, 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 2.2
-
 4-methylumbelliferyl beta-D-galactoside pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 2.2
-
 4-methylumbelliferyl beta-D-galactoside pH 6.5, 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 2.72
-
 4-methylumbelliferyl beta-D-galacturonate pH 6.5, 45°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 3.46
-
 4-methylumbelliferyl beta-D-galacturonate pH 6.5, 45°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 5.1
-
 4-nitrophenyl beta-D-xyloside pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 5.1
-
 4-methylumbelliferyl beta-D-xyloside pH 6.5, 45°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 5.28
-
 4-nitrophenyl beta-D-galactoside pH 6.5 (50 mM phosphate), 45°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 5.28
-
 4-methylumbelliferyl beta-D-galactoside pH 6.5, 45°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 13.7
-
 4-nitrophenyl beta-D-xyloside pH 6.5 (50 mM phosphate), 45°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 13.7
-
 4-methylumbelliferyl beta-D-xyloside pH 6.5, 45°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 18.6
-
 4-nitrophenyl beta-D-fucoside pH 6.5 (50 mM phosphate), 45°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 18.6
-
 4-methylumbelliferyl beta-D-fucoside pH 6.5, 45°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 22.1
-
 4-nitrophenyl beta-D-glucoside pH 6.5 (50 mM phosphate), 45°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 22.1
-
 4-methylumbelliferyl beta-D-glucoside pH 6.5, 45°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 24.2
-
 4-nitrophenyl beta-D-mannoside pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 24.2
-
 4-methylumbelliferyl beta-D-mannoside pH 6.5, 45°C, wild-type enzyme Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B26 Saccharolobus solfataricus P22498
-
-
3.2.1.B26 Saccharolobus solfataricus P2 P22498
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.B26
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B26 4-methylumbelliferyl beta-D-fucoside + H2O
-
 Saccharolobus solfataricus 4-methylumbelliferol + D-fucose
-
 ?
3.2.1.B26 4-methylumbelliferyl beta-D-fucoside + H2O
-
 Saccharolobus solfataricus 4-methylumbelliferone + beta-D-fucose
-
 ?
3.2.1.B26 4-methylumbelliferyl beta-D-galactoside + H2O
-
 Saccharolobus solfataricus 4-methylumbelliferone + beta-D-galactose
-
 ?
3.2.1.B26 4-methylumbelliferyl beta-D-galactoside + H2O
-
 Saccharolobus solfataricus P2 4-methylumbelliferone + beta-D-galactose
-
 ?
3.2.1.B26 4-methylumbelliferyl beta-D-galacturonate + H2O
-
 Saccharolobus solfataricus 4-methylumbelliferone + beta-D-galacturonate
-
 ?
3.2.1.B26 4-methylumbelliferyl beta-D-glucoside + H2O
-
 Saccharolobus solfataricus 4-methylumbelliferone + beta-D-glucose
-
 ?
3.2.1.B26 4-methylumbelliferyl beta-D-glucoside + H2O
-
 Saccharolobus solfataricus P2 4-methylumbelliferone + beta-D-glucose
-
 ?
3.2.1.B26 4-methylumbelliferyl beta-D-glucoside + H2O
-
 Saccharolobus solfataricus 4-methylumbelliferol + D-glucose
-
 ?
3.2.1.B26 4-methylumbelliferyl beta-D-glucoside + H2O
-
 Saccharolobus solfataricus P2 4-methylumbelliferol + D-glucose
-
 ?
3.2.1.B26 4-methylumbelliferyl beta-D-glucuronide + H2O
-
 Saccharolobus solfataricus 4-methylumbelliferol + D-glucose
-
 ?
3.2.1.B26 4-methylumbelliferyl beta-D-mannoside + H2O
-
 Saccharolobus solfataricus 4-methylumbelliferone + beta-D-mannose
-
 ?
3.2.1.B26 4-methylumbelliferyl beta-D-mannoside + H2O
-
 Saccharolobus solfataricus P2 4-methylumbelliferone + beta-D-mannose
-
 ?
3.2.1.B26 4-methylumbelliferyl beta-D-mannoside + H2O
-
 Saccharolobus solfataricus 4-methylumbelliferol + D-mannose
-
 ?
3.2.1.B26 4-methylumbelliferyl beta-D-xyloside + H2O
-
 Saccharolobus solfataricus 4-methylumbelliferol + D-xylose
-
 ?
3.2.1.B26 4-methylumbelliferyl beta-D-xyloside + H2O
-
 Saccharolobus solfataricus 4-methylumbelliferone + beta-D-xylose
-
 ?
3.2.1.B26 4-nitrophenyl beta-D-fucoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + D-fucose
-
 ?
3.2.1.B26 4-nitrophenyl beta-D-galactoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.B26 4-nitrophenyl beta-D-galactoside + H2O
-
 Saccharolobus solfataricus P2 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.B26 4-nitrophenyl beta-D-glucoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenyl + D-glucose
-
 ?
3.2.1.B26 4-nitrophenyl beta-D-glucuronide + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + D-glucose
-
 ?
3.2.1.B26 4-nitrophenyl beta-D-mannoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + D-mannose
-
 ?
3.2.1.B26 4-nitrophenyl beta-D-xyloside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + D-xylose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.B26 LACS
-
 Saccharolobus solfataricus
3.2.1.B26 SsbetaG
-
 Saccharolobus solfataricus
3.2.1.B26 SSO3019 locus name Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B26 80
-
 assay at Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.B26 0.004
-
 4-methylumbelliferyl beta-D-galacturonate pH 6.5, 45°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.004
-
 4-methylumbelliferyl beta-D-galacturonate pH 6.5, 45°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 0.018
-
 4-nitrophenyl beta-D-glucoside pH 6.5 (50 mM phosphate), 45°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.018
-
 4-methylumbelliferyl beta-D-glucoside pH 6.5, 45°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.028
-
 4-nitrophenyl beta-D-xyloside pH 6.5 (50 mM phosphate), 45°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.028
-
 4-methylumbelliferyl beta-D-xyloside pH 6.5, 45°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.047
-
 4-nitrophenyl beta-D-galactoside pH 6.5 (50 mM phosphate), 45°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.047
-
 4-methylumbelliferyl beta-D-galactoside pH 6.5, 45°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.08
-
 4-nitrophenyl beta-D-galactoside pH 6.5 (50 mM phosphate), 45°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 0.08
-
 4-methylumbelliferyl beta-D-galactoside pH 6.5, 45°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 0.81
-
 4-methylumbelliferyl beta-D-glucuronide pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.81
-
 4-methylumbelliferyl beta-D-galacturonate pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.92
-
 4-methylumbelliferyl beta-D-mannoside pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 0.92
-
 4-methylumbelliferyl beta-D-mannoside pH 6.5, 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 1.5
-
 4-methylumbelliferyl beta-D-xyloside pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 1.5
-
 4-methylumbelliferyl beta-D-xyloside pH 6.5, 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 1.8
-
 4-methylumbelliferyl beta-D-mannoside pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 1.8
-
 4-methylumbelliferyl beta-D-mannoside pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 2.65
-
 4-nitrophenyl beta-D-mannoside pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 2.65
-
 4-methylumbelliferyl beta-D-mannoside pH 6.5, 45°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 2.72
-
 4-nitrophenyl beta-D-glucoside pH 6.5 (50 mM phosphate), 45°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 2.72
-
 4-methylumbelliferyl beta-D-glucoside pH 6.5, 45°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 2.8
-
 4-methylumbelliferyl beta-D-xyloside pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 2.8
-
 4-methylumbelliferyl beta-D-mannoside pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 2.8
-
 4-methylumbelliferyl beta-D-xyloside pH 6.5, 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 2.8
-
 4-methylumbelliferyl beta-D-mannoside pH 6.5, 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 3.8
-
 4-methylumbelliferyl beta-D-xyloside pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 3.8
-
 4-methylumbelliferyl beta-D-xyloside pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 3.95
-
 4-nitrophenyl beta-D-xyloside pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 3.95
-
 4-methylumbelliferyl beta-D-xyloside pH 6.5, 45°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 4.35
-
 4-nitrophenyl beta-D-glucoside pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 4.35
-
 4-methylumbelliferyl beta-D-glucoside pH 6.5, 45°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 5.1
-
 4-methylumbelliferyl beta-D-glucoside pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 5.1
-
 4-methylumbelliferyl beta-D-glucoside pH 6.5, 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 5.16
-
 4-nitrophenyl beta-D-fucoside pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 5.16
-
 4-methylumbelliferyl beta-D-fucoside pH 6.5, 45°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 5.4
-
 4-methylumbelliferyl beta-D-galactoside pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 5.4
-
 4-methylumbelliferyl beta-D-galactoside pH 6.5, 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 7.2
-
 4-nitrophenyl beta-D-galactoside pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 7.2
-
 4-methylumbelliferyl beta-D-galactoside pH 6.5, 45°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 9.43
-
 4-nitrophenyl beta-D-fucoside pH 6.5 (50 mM phosphate), 45°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 9.43
-
 4-methylumbelliferyl beta-D-fucoside pH 6.5, 45°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 14.6
-
 4-methylumbelliferyl beta-D-galactoside pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 14.6
-
 4-methylumbelliferyl beta-D-galactoside pH 6.5, 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 18
-
 4-methylumbelliferyl beta-D-fucoside pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 18
-
 4-methylumbelliferyl beta-D-fucoside pH 6.5, 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 31
-
 4-methylumbelliferyl beta-D-fucoside pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 31
-
 4-methylumbelliferyl beta-D-fucoside pH 6.5, 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 33
-
 4-methylumbelliferyl beta-D-glucoside pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 33
-
 4-methylumbelliferyl beta-D-glucoside pH 6.5, 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 80
-
 4-methylumbelliferyl beta-D-fucoside pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 89
-
 4-methylumbelliferyl beta-D-fucoside pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 98
-
 4-methylumbelliferyl beta-D-galactoside pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 98
-
 4-methylumbelliferyl beta-D-galactoside pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 140
-
 4-methylumbelliferyl beta-D-glucoside pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 140
-
 4-methylumbelliferyl beta-D-glucoside pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.B26 6.5
-
 assay at Saccharolobus solfataricus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.B26 0.0006
-
 4-methylumbelliferyl beta-D-galacturonate pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.001
-
 4-nitrophenyl beta-D-mannoside pH 6.5 (50 mM phosphate), 45°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.001
-
 4-methylumbelliferyl beta-D-mannoside pH 6.5, 45°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.001
-
 4-methylumbelliferyl beta-D-galacturonate pH 6.5, 45°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.0013
-
 4-methylumbelliferyl beta-D-galacturonate pH 6.5, 45°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 0.002
-
 4-nitrophenyl beta-D-xyloside pH 6.5 (50 mM phosphate), 45°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.002
-
 4-methylumbelliferyl beta-D-xyloside pH 6.5, 45°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.0022
-
 4-methylumbelliferyl beta-D-xyloside pH 6.5, 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.0025
-
 4-methylumbelliferyl beta-D-xyloside pH 6.5, 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 0.003
-
 4-nitrophenyl beta-D-xyloside pH 6.5 (50 mM phosphate), 45°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 0.003
-
 4-methylumbelliferyl beta-D-xyloside pH 6.5, 45°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 0.0032
-
 4-methylumbelliferyl beta-D-mannoside pH 6.5, 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.005
-
 4-nitrophenyl beta-D-mannoside pH 6.5 (50 mM phosphate), 45°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 0.005
-
 4-methylumbelliferyl beta-D-mannoside pH 6.5, 45°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 0.0051
-
 4-methylumbelliferyl beta-D-mannoside pH 6.5, 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 0.0063
-
 4-methylumbelliferyl beta-D-galactoside pH 6.5, 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 0.011
-
 4-methylumbelliferyl beta-D-galactoside pH 6.5, 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.015
-
 4-nitrophenyl beta-D-galactoside pH 6.5 (50 mM phosphate), 45°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 0.015
-
 4-methylumbelliferyl beta-D-galactoside pH 6.5, 45°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 0.015
-
 4-methylumbelliferyl beta-D-glucoside pH 6.5, 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.02
-
 4-methylumbelliferyl beta-D-glucoside pH 6.5, 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 0.03
-
 4-methylumbelliferyl beta-D-xyloside pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.036
-
 4-nitrophenyl beta-D-glucoside pH 6.5 (50 mM phosphate), 45°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.036
-
 4-methylumbelliferyl beta-D-glucoside pH 6.5, 45°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.05
-
 4-methylumbelliferyl beta-D-mannoside pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.053
-
 4-methylumbelliferyl beta-D-fucoside pH 6.5, 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.058
-
 4-nitrophenyl beta-D-galactoside pH 6.5 (50 mM phosphate), 45°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.058
-
 4-methylumbelliferyl beta-D-galactoside pH 6.5, 45°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.06
-
 4-methylumbelliferyl beta-D-galacturonate pH 6.5, 45°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.068
-
 4-nitrophenyl beta-D-fucoside pH 6.5 (50 mM phosphate), 45°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.068
-
 4-methylumbelliferyl beta-D-fucoside pH 6.5, 45°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.076
-
 4-methylumbelliferyl beta-D-fucoside pH 6.5, 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 0.11
-
 4-nitrophenyl beta-D-mannoside pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.11
-
 4-methylumbelliferyl beta-D-mannoside pH 6.5, 45°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.123
-
 4-nitrophenyl beta-D-glucoside pH 6.5 (50 mM phosphate), 45°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 0.123
-
 4-methylumbelliferyl beta-D-glucoside pH 6.5, 45°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 0.506
-
 4-nitrophenyl beta-D-fucoside pH 6.5 (50 mM phosphate), 45°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 0.506
-
 4-methylumbelliferyl beta-D-fucoside pH 6.5, 45°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 0.6
-
 4-methylumbelliferyl beta-D-galactoside pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 0.6
-
 4-methylumbelliferyl beta-D-glucuronide pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.77
-
 4-nitrophenyl beta-D-xyloside pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.77
-
 4-methylumbelliferyl beta-D-xyloside pH 6.5, 45°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 1.49
-
 4-methylumbelliferyl beta-D-galactoside pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 2.2
-
 4-methylumbelliferyl beta-D-xyloside pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 2.5
-
 4-methylumbelliferyl beta-D-fucoside pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 2.9
-
 4-methylumbelliferyl beta-D-glucoside pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 3.2
-
 4-methylumbelliferyl beta-D-mannoside pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 5.1
-
 4-methylumbelliferyl beta-D-glucuronide pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 6.3
-
 4-methylumbelliferyl beta-D-glucoside pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 7.3
-
 4-methylumbelliferyl beta-D-fucoside pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 11
-
 4-methylumbelliferyl beta-D-galactoside pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 12.7
-
 4-nitrophenyl beta-D-galactoside pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 12.7
-
 4-methylumbelliferyl beta-D-galactoside pH 6.5, 45°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 15
-
 4-methylumbelliferyl beta-D-glucoside pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 20
-
 4-methylumbelliferyl beta-D-xyloside pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 29.8
-
 4-nitrophenyl beta-D-glucoside pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 29.8
-
 4-methylumbelliferyl beta-D-glucoside pH 6.5, 45°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 30
-
 4-methylumbelliferyl beta-D-xyloside pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 42.6
-
 4-nitrophenyl beta-D-fucoside pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 42.6
-
 4-methylumbelliferyl beta-D-fucoside pH 6.5, 45°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 50
-
 4-methylumbelliferyl beta-D-mannoside pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 53
-
 4-methylumbelliferyl beta-D-fucoside pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 76
-
 4-methylumbelliferyl beta-D-mannoside pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 1490
-
 4-methylumbelliferyl beta-D-galactoside pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 2900
-
 4-methylumbelliferyl beta-D-glucoside pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 7300
-
 4-methylumbelliferyl beta-D-fucoside pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme Saccharolobus solfataricus