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Literature summary extracted from

  • Crona, F.; Dahlberg, O.; Lundberg, L.E.; Larsson, J.; Mannervik, M.
    Gene regulation by the lysine demethylase KDM4A in Drosophila (2013), Dev. Biol., 373, 453-463.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.69 characterization of dKDM4A alleles, and the dKDM4A genomic locus, the dKDM4A locus contains four exons, overview. Quantitative RT-PCR of dKDM4A expression analysis of wild-type and mutant enzymes in larvae Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
1.14.11.69 H195A site-directed mutagenesis Drosophila melanogaster
1.14.11.69 additional information generation of JmjC protein dKDM4A mutant allele, DELTAdKDM4A, with a deletion spanning from 47 nucleotides downstream of the ATG to the end of the gene by means of imprecise excision of the P-element P{GawB}NP0618 Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.11.69 nucleus
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 Drosophila melanogaster 5634
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Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.69 histone H3-N6,N6,N6-trimethyl-L-lysine36 + 2-oxoglutarate + O2 Drosophila melanogaster KDM4A demethylates H3K36me3, a modification enriched in the 3' end of active genes histone H3-N6,N6-dimethyl-L-lysine36 + succinate + formaldehyde + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.69 Drosophila melanogaster
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 gene dkdm4a
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.69 larva quantitative RT-PCRof dKDM4A expression analysis of wild-type and mutant enzymes in larvae Drosophila melanogaster
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.69 histone H3-N6,N6,N6-trimethyl-L-lysine36 + 2-oxoglutarate + O2
-
 Drosophila melanogaster histone H3-N6,N6-dimethyl-L-lysine36 + succinate + formaldehyde + CO2
-
 ?
1.14.11.69 histone H3-N6,N6,N6-trimethyl-L-lysine36 + 2-oxoglutarate + O2 KDM4A demethylates H3K36me3, a modification enriched in the 3' end of active genes Drosophila melanogaster histone H3-N6,N6-dimethyl-L-lysine36 + succinate + formaldehyde + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.69 JmjC protein
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 Drosophila melanogaster
1.14.11.69 KDM4
-
 Drosophila melanogaster
1.14.11.69 KDM4A demethylase
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 Drosophila melanogaster

General Information

EC Number General Information Comment Organism
1.14.11.69 malfunction enzyme mutants show 99 misregulated genes in first instar larvae. dKDM4A overexpression results in a global decrease in H3K36me3 levels and male lethality, which might be caused by impaired dosage compensation Drosophila melanogaster
1.14.11.69 additional information a modest increase in global H3K36me3 levels is compatible with viability, fertility, and the expression of most genes, whereas decreased H3K36me3 levels are detrimental in males Drosophila melanogaster
1.14.11.69 physiological function identification of dKDM4A-regulated genes, overview. Appropriate expression levels for some dKDM4A-regulated genes rely on the demethylase activity of dKDM4A, whereas others do not. Highly expressed, many demethylase-dependent and independent genes are devoid of H3K36me3 in wild-type as well as in dKDM4A mutant larvae. Some of the most strongly affected genes in dKDM4A mutant animals are not regulated by H3K36 methylation Drosophila melanogaster