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Literature summary extracted from

  • de Medeiros, P.S.; Ducati, R.G.; Basso, L.A.; Santos, D.S.; da Silva, L.H.
    Enzyme mechanism and slow-onset inhibition of Plasmodium falciparum enoyl-acyl carrier protein reductase by an inorganic complex (2011), Enzyme Res., 2011, 642758.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.9 pentacyano (isoniazid)ferrate-II [FeII(CN)5(INH)]3+, slow onset inhibitor Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.9 0.063
-
 crotonyl-CoA pH 7.5, 25°C Plasmodium falciparum
1.3.1.9 0.085
-
 NADH pH 7.5, 25°C Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.9 Plasmodium falciparum
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.1.9 an acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+ double displacement mechanism. In this mechanism, a catalytic event occurs after the binding of NADH to free enzyme, generating and releasing NAD+ into solution prior to the binding of crotonoyl-CoA to a modified form of the enzyme, followed by formation of butyryl-CoA and regeneration of free enzyme Plasmodium falciparum
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.9 crotonyl-CoA + NADH + H+
-
 Plasmodium falciparum butyryl-CoA + NAD+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.9 6.2
-
 crotonyl-CoA pH 7.5, 25°C Plasmodium falciparum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.1.9 0.04
-
 pentacyano (isoniazid)ferrate-II pH 7.5, 25°C Plasmodium falciparum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3.1.9 73
-
 NADH pH 7.5, 25°C Plasmodium falciparum
1.3.1.9 98
-
 crotonyl-CoA pH 7.5, 25°C Plasmodium falciparum