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Literature summary extracted from

  • Koerdt, A.; Jachlewski, S.; Ghosh, A.; Wingender, J.; Siebers, B.; Albers, S.V.
    Complementation of Sulfolobus solfataricus PBL2025 with an alpha-mannosidase: effects on surface attachment and biofilm formation (2012), Extremophiles, 16, 115-125.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.23 overexpression in Sulfolobus solfataricus mutant PBL2025, that misses 50 genes (SSO3004-3050), including genes coding for a multitude of enzymes possibly involved in sugar degradation or metabolism Saccharolobus solfataricus
3.2.1.24 overexpression in Sulfolobus solfataricus mutant PBL2025, that misses 50 genes (SSO3004-3050), including genes coding for a multitude of enzymes possibly involved in sugar degradation or metabolism Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Saccharolobus solfataricus P22498
-
-
3.2.1.23 Saccharolobus solfataricus P2 P22498
-
-
3.2.1.24 Saccharolobus solfataricus Q97UK5
-
-
3.2.1.24 Saccharolobus solfataricus P2 Q97UK5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23
-
 Saccharolobus solfataricus
3.2.1.24
-
 Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.23 SSO3019 locus name Saccharolobus solfataricus
3.2.1.24 SSO3006 locus name Saccharolobus solfataricus

General Information

EC Number General Information Comment Organism
3.2.1.23 physiological function mutant PBL2025, that misses 50 genes (SSO3004-3050), including genes coding for a multitude of enzymes possibly involved in sugar degradation or metabolism, is complemented with one of the missing proteins, the beta-galactosidase (SSO3019). The beta-galactosidase complemented strain shows reduced carbohydrate content of the isolated total extracellular polymeric substance implying a role in the modulation of the produced EPS during static biofilm formation Saccharolobus solfataricus
3.2.1.24 physiological function mutant PBL2025, that misses 50 genes (SSO3004-3050), including genes coding for a multitude of enzymes possibly involved in sugar degradation or metabolism, is complemented with one of the missing proteins, the alpha-mannosidase (SSO3006). The alpha-mannosidase complemented strain resembles the Sulfolobus solfataricus P2 behavior with respect to attachment of cells to glass and growth of cells in static biofilms. During expression of the alpha-mannosidase, glucose and mannose-containing extracellular polymeric substance levels changes in the recombinant strain during surface attachment and biofilm formation. alpha-Mannosidase might be involved in the modulation of the extracellular polymeric substance composition and/or in the de-mannosylation of the glycan tree, which is attached to extracellular glycosylated proteins in Sulfolobus solfataricus Saccharolobus solfataricus