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Literature summary extracted from

  • Hansen, M.R.; Jensen, K.S.; Rasmussen, M.S.; Christoffersen, S.; Kadziola, A.; Jensen, K.F.
    Specificities and pH profiles of adenine and hypoxanthine-guanine-xanthine phosphoribosyltransferases (nucleotide synthases) of the thermoacidophile archaeon Sulfolobus solfataricus (2014), Extremophiles, 18, 179-187.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.7 expressed in Escherichia coli Saccharolobus solfataricus
2.4.2.8 expressed in Escherichia coli Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.7 D99N mutant enzyme has very low activity Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.7 ADP is a much stronger inhibitor than ATP at pH 4.5, but has virtually no effect on activity at neutral pH Saccharolobus solfataricus
2.4.2.7 AMP
-
 Saccharolobus solfataricus
2.4.2.7 ATP
-
 Saccharolobus solfataricus
2.4.2.8 additional information the activity of HGXPRTase was unaffected by the triphosphates ATP, GTP, CTP or UTP Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.7 24200
-
 2 * 24200, SDS-PAGE Saccharolobus solfataricus
2.4.2.7 45000
-
 gel filtration Saccharolobus solfataricus
2.4.2.8 20600
-
 2 * 20600, SDS-PAGE Saccharolobus solfataricus
2.4.2.8 45000
-
 gel filtration Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.7 Saccharolobus solfataricus
-
-
-
2.4.2.7 Saccharolobus solfataricus P2
-
-
-
2.4.2.8 Saccharolobus solfataricus Q97W22
-
-
2.4.2.8 Saccharolobus solfataricus P2 Q97W22
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.7
-
 Saccharolobus solfataricus
2.4.2.8
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate strong preference for adenine as substrate over the 6-oxopurine bases, hypoxanthine and guanine Saccharolobus solfataricus AMP + diphosphate
-
 ?
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate strong preference for adenine as substrate over the 6-oxopurine bases, hypoxanthine and guanine Saccharolobus solfataricus P2 AMP + diphosphate
-
 ?
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Saccharolobus solfataricus GMP + diphosphate
-
 ?
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Saccharolobus solfataricus P2 GMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Saccharolobus solfataricus IMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Saccharolobus solfataricus P2 IMP + diphosphate
-
 ?
2.4.2.8 xanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Saccharolobus solfataricus XMP + diphosphate
-
 ?
2.4.2.8 xanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Saccharolobus solfataricus P2 XMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.7 dimer 2 * 24200, SDS-PAGE Saccharolobus solfataricus
2.4.2.8 dimer 2 * 20600, SDS-PAGE Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
2.4.2.7 adenione PRTase
-
 Saccharolobus solfataricus
2.4.2.7 APRTase
-
 Saccharolobus solfataricus
2.4.2.7 apT
-
 Saccharolobus solfataricus
2.4.2.7 gpT-1
-
 Saccharolobus solfataricus
2.4.2.7 SSO2342
-
 Saccharolobus solfataricus
2.4.2.8 6-oxo PRTase
-
 Saccharolobus solfataricus
2.4.2.8 6-oxopurine PRTase
-
 Saccharolobus solfataricus
2.4.2.8 gpT-2
-
 Saccharolobus solfataricus
2.4.2.8 HGXPRTase
-
 Saccharolobus solfataricus
2.4.2.8 SSO2424
-
 Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.7 4.5
-
 the enzyme shows two pH optima, i.e., around 8 and 4.5. The activity of APRTase is considerably higher at pH 8 than pH 4.5, when assayed at 35°C, while the two activity peaks seems more equal, when assayed at 60°C Saccharolobus solfataricus
2.4.2.7 7.5 8.5 the enzyme shows two pH optima, i.e., around 8 and 4.5. The activity of APRTase is considerably higher at pH 8 than pH 4.5, when assayed at 35°C, while the two activity peaks seems more equal, when assayed at 60°C Saccharolobus solfataricus
2.4.2.8 4.5
-
 maximal activity with hypoxanthine and guanine Saccharolobus solfataricus
2.4.2.8 7.5
-
 maximal activity with xanthine Saccharolobus solfataricus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.7 0.019
-
 ADP 60°C, pH 4.1 Saccharolobus solfataricus
2.4.2.7 0.177
-
 AMP 60°C, pH 4.1 Saccharolobus solfataricus
2.4.2.7 0.3
-
 AMP 60°C, pH 7.5 Saccharolobus solfataricus
2.4.2.7 0.344
-
 ATP 60°C, pH 4.1 Saccharolobus solfataricus
2.4.2.7 0.574
-
 ATP 60°C, pH 7.5 Saccharolobus solfataricus
2.4.2.7 3.2
-
 ADP 60°C, pH 7.5 Saccharolobus solfataricus