EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.7.2 | expression in Escherichia coli DH5alpha | Methanosarcina mazei |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.7.2 | 0.0005 | - |
reduced ferredoxin | pH 7.0, temperature not specified in the publication | Methanosarcina mazei | |
1.5.7.2 | 0.002 | - |
oxidized coenzyme F420 | pH 7.0, temperature not specified in the publication | Methanosarcina mazei |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.5.7.2 | cytoplasm | as a soluble single subunit it is involved in the reoxidation of reduced ferredoxin in the cytoplasm, whereby reduced F420 is produced that is then reoxidized by the complete Fpo complex | Methanosarcina mazei | 5737 | - |
1.5.7.2 | membrane | when the Fpo protein is connected to the membrane integral Fpo complex, it functions as an electron input module of the Fpo complex | Methanosarcina mazei | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.5.7.2 | Iron-sulfur cluster | presence of two FeS clusters, 8.7 mol of nonheme iron and 5.1 mol of acid-labile sulfur per mol of protein | Methanosarcina mazei |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.7.2 | oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+ | Methanosarcina mazei | FpoF can function as an ferredoxin:F420 oxidoreductase when reduced ferredoxin accumulates in the cytoplasm. As a soluble single subunit it is involved in the reoxidation of reduced ferredoxin in the cytoplasm, whereby reduced F420 is produced that is then reoxidized by the complete Fpo complex | reduced coenzyme F420 + 2 oxidized ferredoxin | - |
r | |
1.5.7.2 | oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+ | Methanosarcina mazei DSM 3647 | FpoF can function as an ferredoxin:F420 oxidoreductase when reduced ferredoxin accumulates in the cytoplasm. As a soluble single subunit it is involved in the reoxidation of reduced ferredoxin in the cytoplasm, whereby reduced F420 is produced that is then reoxidized by the complete Fpo complex | reduced coenzyme F420 + 2 oxidized ferredoxin | - |
r | |
1.5.7.2 | reduced coenzyme F420 + 2 oxidized ferredoxin | Methanosarcina mazei | when the Fpo protein is connected to the membrane integral Fpo complex, it functions as an electron input module of the Fpo complex | oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+ | - |
r | |
1.5.7.2 | reduced coenzyme F420 + 2 oxidized ferredoxin | Methanosarcina mazei DSM 3647 | when the Fpo protein is connected to the membrane integral Fpo complex, it functions as an electron input module of the Fpo complex | oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.7.2 | Methanosarcina mazei | Q8PZ67 | - |
- |
1.5.7.2 | Methanosarcina mazei DSM 3647 | Q8PZ67 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.7.2 | - |
Methanosarcina mazei |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.7.2 | oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+ | - |
Methanosarcina mazei | reduced coenzyme F420 + 2 oxidized ferredoxin | - |
r | |
1.5.7.2 | oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+ | FpoF can function as an ferredoxin:F420 oxidoreductase when reduced ferredoxin accumulates in the cytoplasm. As a soluble single subunit it is involved in the reoxidation of reduced ferredoxin in the cytoplasm, whereby reduced F420 is produced that is then reoxidized by the complete Fpo complex | Methanosarcina mazei | reduced coenzyme F420 + 2 oxidized ferredoxin | - |
r | |
1.5.7.2 | oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+ | - |
Methanosarcina mazei DSM 3647 | reduced coenzyme F420 + 2 oxidized ferredoxin | - |
r | |
1.5.7.2 | oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+ | FpoF can function as an ferredoxin:F420 oxidoreductase when reduced ferredoxin accumulates in the cytoplasm. As a soluble single subunit it is involved in the reoxidation of reduced ferredoxin in the cytoplasm, whereby reduced F420 is produced that is then reoxidized by the complete Fpo complex | Methanosarcina mazei DSM 3647 | reduced coenzyme F420 + 2 oxidized ferredoxin | - |
r | |
1.5.7.2 | reduced coenzyme F420 + 2 oxidized ferredoxin | when the Fpo protein is connected to the membrane integral Fpo complex, it functions as an electron input module of the Fpo complex | Methanosarcina mazei | oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+ | - |
r | |
1.5.7.2 | reduced coenzyme F420 + 2 oxidized ferredoxin | when the Fpo protein is connected to the membrane integral Fpo complex, it functions as an electron input module of the Fpo complex | Methanosarcina mazei DSM 3647 | oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+ | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.7.2 | Fd:F420 oxidoreductase | - |
Methanosarcina mazei |
1.5.7.2 | FpoF protein | - |
Methanosarcina mazei |
1.5.7.2 | mm0627 | - |
Methanosarcina mazei |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.7.2 | 7 | - |
assay at | Methanosarcina mazei |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.7.2 | FAD | presence of 1.0 mol FAD per mol protein | Methanosarcina mazei |