Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Blee, E.; Flenet, M.; Boachon, B.; Fauconnier, M.L.
    A non-canonical caleosin from Arabidopsis efficiently epoxidizes physiological unsaturated fatty acids with complete stereoselectivity (2012), FEBS J., 279, 3981-3995.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.2.3 expression in Saccharomyces cerevisiae Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.2.3 membrane associated with membrane Arabidopsis thaliana 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.2.3 Ca2+ required Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.2.3 24000
-
 x * 24000, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.2.3 linoleic acid + cumene hydroperoxide Arabidopsis thaliana
-
 ? 64% conversion, physiological linoleic and linolenic acids are the preferred substrates for isoform PXG4 ?
1.11.2.3 linolenic acid + cumene hydroperoxide Arabidopsis thaliana 74% conversion, physiological linoleic and linolenic acids are the preferred substrates for isoform PXG4 linolenic acid 9,10-15,16-diepoxide + ? major product, (R),(S)-epoxide enantiomers ?
1.11.2.3 additional information Arabidopsis thaliana enzyme is an efficient fatty acid epoxygenase, catalyzing the oxidation of cis double bonds of unsaturated fatty acids. The C-12,13 double bond of these unsaturated fatty acids is the least favoured. Isoform PXG4 catalyzes exclusively the formation of (R),(S)-epoxide enantiomers, which is the absolute stereochemistry of the epoxides found in planta. Poor substrate: oleic acid methyl ester ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.2.3 Arabidopsis thaliana Q9CAB7
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.2.3 flower expression in root, stem, leaf and flower Arabidopsis thaliana
-
1.11.2.3 leaf expression in root, stem, leaf and flower Arabidopsis thaliana
-
1.11.2.3 root expression in root, stem, leaf and flower Arabidopsis thaliana
-
1.11.2.3 stem expression in root, stem, leaf and flower Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.2.3 linoleic acid + cumene hydroperoxide
-
 Arabidopsis thaliana ? 64% conversion, physiological linoleic and linolenic acids are the preferred substrates for isoform PXG4 ?
1.11.2.3 linolenic acid + cumene hydroperoxide 74% conversion, physiological linoleic and linolenic acids are the preferred substrates for isoform PXG4 Arabidopsis thaliana linolenic acid 9,10-15,16-diepoxide + ? major product, (R),(S)-epoxide enantiomers ?
1.11.2.3 additional information enzyme is an efficient fatty acid epoxygenase, catalyzing the oxidation of cis double bonds of unsaturated fatty acids. The C-12,13 double bond of these unsaturated fatty acids is the least favoured. Isoform PXG4 catalyzes exclusively the formation of (R),(S)-epoxide enantiomers, which is the absolute stereochemistry of the epoxides found in planta. Poor substrate: oleic acid methyl ester Arabidopsis thaliana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.2.3 ? x * 24000, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.11.2.3 PXG4
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.2.3 heme contains a protoheme of type b, with 0.6 nmol of heme per mg protein Arabidopsis thaliana