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Literature summary extracted from

  • Knauer, S.H.; Hartl-Spiegelhauer, O.; Schwarzinger, S.; Haenzelmann, P.; Dobbek, H.
    The Fe(II)/alpha-ketoglutarate-dependent taurine dioxygenases from Pseudomonas putida and Escherichia coli are tetramers (2012), FEBS J., 279, 816-831.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.17 expression of TauD in Escherichia coli strains BL21(DE3) and Rosetta Blue(DE3) Pseudomonas putida

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.11.17 three crystal structures of the apo form, vapor diffusion techniques, protein solution contains 1. 27-28 m/ml TauDPp in 25 mM Tris-HCl, pH 7.7, or 2. 21 mg/ml TauDPp in 25 mM Tris-HCl pH 7.7, containing 10 mM taurine, 0.1 M (NH4)2SO4 and 20% v?v glycerol, mixed with reservoir solution containing 1. 15% w?v PEG 1000, 40% v?v PEG 400, 0.15 M NaK phosphate, and 0.1 M imidazole chloride, pH 6.5, or 2. 20% w?v PEG 5000 monomethyl ether, 0.1 M Bis-Tris-HCl, pH 6.5, X-ray diffraction structure determination and analysis at 1.85-2.6 A resolution Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.17 Fe2+ dependent on, mononuclear non-heme iron center, binding structure and kinetics, spectral analysis, overview Escherichia coli
1.14.11.17 Fe2+ dependent on, mononuclear non-heme iron center, binding structure and kinetics, spectral analysis, overview Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.17 31000
-
 4 * 31000, about, sequence calculation, TauDPp is a tetramer in solution and in the crystals Pseudomonas putida
1.14.11.17 32000
-
 4 * 32000, about, sequence calculation, TauDEc is a tetramer in solution and in the crystals, gel filtration and sedimentation velocity analytical ultracentrifugation Escherichia coli
1.14.11.17 110000
-
 gel filtration Escherichia coli
1.14.11.17 112000
-
 gel filtration Pseudomonas putida
1.14.11.17 120000
-
 sedimentation velocity analytical ultracentrifugation Pseudomonas putida
1.14.11.17 127000
-
 sedimentation velocity analytical ultracentrifugation Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.17 taurine + 2-oxoglutarate + O2 Escherichia coli
-
 sulfite + aminoacetaldehyde + succinate + CO2
-
 ?
1.14.11.17 taurine + 2-oxoglutarate + O2 Pseudomonas putida
-
 sulfite + aminoacetaldehyde + succinate + CO2
-
 ?
1.14.11.17 taurine + 2-oxoglutarate + O2 Pseudomonas putida KT 2240
-
 sulfite + aminoacetaldehyde + succinate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.17 Escherichia coli
-
-
-
1.14.11.17 Pseudomonas putida Q88RA3
-
-
1.14.11.17 Pseudomonas putida KT 2240 Q88RA3
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.11.17 3.56
-
 purified enzyme, pH and temperature not specified in the publication Escherichia coli
1.14.11.17 8.11
-
 purified enzyme, pH and temperature not specified in the publication Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.17 taurine + 2-oxoglutarate + O2
-
 Escherichia coli sulfite + aminoacetaldehyde + succinate + CO2
-
 ?
1.14.11.17 taurine + 2-oxoglutarate + O2
-
 Pseudomonas putida sulfite + aminoacetaldehyde + succinate + CO2
-
 ?
1.14.11.17 taurine + 2-oxoglutarate + O2
-
 Pseudomonas putida KT 2240 sulfite + aminoacetaldehyde + succinate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.17 More topology of an apo-TauDPp monomer,overview Pseudomonas putida
1.14.11.17 tetramer 4 * 31000, about, sequence calculation, TauDPp is a tetramer in solution and in the crystals Pseudomonas putida
1.14.11.17 tetramer 4 * 32000, about, sequence calculation, TauDEc is a tetramer in solution and in the crystals, gel filtration and sedimentation velocity analytical ultracentrifugation Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.14.11.17 Fe(II)/alpha-ketoglutarate-dependent taurine dioxygenase
-
 Escherichia coli
1.14.11.17 Fe(II)/alpha-ketoglutarate-dependent taurine dioxygenase
-
 Pseudomonas putida
1.14.11.17 TauD
-
 Escherichia coli
1.14.11.17 TauD
-
 Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.11.17 additional information
-
 additional information real-time NMR spectra to follow enzymatic turnover Pseudomonas putida

General Information

EC Number General Information Comment Organism
1.14.11.17 additional information metal-to-ligand charge-transfer transition involving the 2-oxoglutarate ligand with a six-coordinate metal center. Taurine does not coordinate the Fe(II) ion, but binds in its close vicinity, leading to loss of the coordinating water molecule. This change of the six-coordinate to five-coordinate Fe(II) center causes a small perturbation of the absorption spectrum Escherichia coli
1.14.11.17 additional information metal-to-ligand charge-transfer transition involving the 2-oxoglutarate ligand with a six-coordinate metal center. Taurine does not coordinate the Fe(II) ion, but binds in its close vicinity, leading to loss of the coordinating water molecule. This change of the six-coordinate to five-coordinate Fe(II) center causes a small perturbation of the absorption spectrum Pseudomonas putida