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Literature summary extracted from

  • Kouril, T.; Esser, D.; Kort J.; Westerhoff, H.V.; Siebers, B.; Snoep, J.L.
    Intermediate instability at high temperature leads to low pathway efficiency for an in vitro reconstituted system of gluconeogenesis in Sulfolobus solfataricus (2013), FEBS J., 280, 4666-4680.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.13 expression in Escherichia coli Saccharolobus solfataricus
2.7.2.3 expression in Escherichia coli Saccharolobus solfataricus
4.1.2.13 expression in Escherichia coli Saccharolobus solfataricus
5.3.1.1 expression in Escherichia coli Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.3 ADP
-
 Saccharolobus solfataricus
5.3.1.1 3-phospho-D-glycerate
-
 Saccharolobus solfataricus
5.3.1.1 phosphoenolpyruvate
-
 Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.13 0.00041
-
 3-phospho-D-glyceroyl phosphate pH 6.5, 70°C Saccharolobus solfataricus
1.2.1.13 0.074
-
 NADPH pH 6.5, 70°C Saccharolobus solfataricus
1.2.1.13 0.271
-
 NADP+ pH 6.5, 70°C Saccharolobus solfataricus
1.2.1.13 0.838
-
 D-glyceraldehyde 3-phosphate pH 6.5, 70°C Saccharolobus solfataricus
1.2.1.13 409
-
 phosphate pH 6.5, 70°C Saccharolobus solfataricus
2.7.2.3 additional information
-
 additional information the enzyme shows hyperbolic saturation kinetics for 3-phospho-D-glycerate, ATP and 3-phospho-D-glyceroyl phosphate. For ADP it shows a high affinity binding for the reaction in the reverse direction and a low affinity inhibitory effect for the forward as well as the reverse reaction Saccharolobus solfataricus
2.7.2.3 0.085
-
 ADP 70°C, pH 6.5 Saccharolobus solfataricus
2.7.2.3 0.54
-
 3-phospho-D-glycerate 70°C, pH 6.5 Saccharolobus solfataricus
2.7.2.3 5.6
-
 3-phospho-D-glyceroyl phosphate 70°C, pH 6.5 Saccharolobus solfataricus
2.7.2.3 9.7
-
 ATP 70°C, pH 6.5 Saccharolobus solfataricus
4.1.2.13 0.052
-
 D-glyceraldehyde 3-phosphate pH 6.5, 70°C Saccharolobus solfataricus
4.1.2.13 0.171
-
 glycerone phosphate pH 6.5, 70°C Saccharolobus solfataricus
5.3.1.1 0.245
-
 D-glyceraldehyde 3-phosphate pH 6.5, 70°C Saccharolobus solfataricus
5.3.1.1 0.812
-
 glycerone phosphate pH 6.5, 70°C Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.13 38000
-
 SDS-PAGE Saccharolobus solfataricus
5.3.1.1 25000
-
 SDS-PAGE Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.13 Saccharolobus solfataricus P39460
-
-
1.2.1.13 Saccharolobus solfataricus P2 P39460
-
-
2.7.2.3 Saccharolobus solfataricus P50317
-
-
2.7.2.3 Saccharolobus solfataricus P2 P50317
-
-
4.1.2.13 Saccharolobus solfataricus Q980K6
-
-
4.1.2.13 Saccharolobus solfataricus P2 Q980K6
-
-
5.3.1.1 Saccharolobus solfataricus Q97VM8
-
-
5.3.1.1 Saccharolobus solfataricus P2 Q97VM8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.13
-
 Saccharolobus solfataricus
2.7.2.3
-
 Saccharolobus solfataricus
4.1.2.13
-
 Saccharolobus solfataricus
5.3.1.1
-
 Saccharolobus solfataricus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.2.3 ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate an ordered mechanism is assumed where 3-phospho-D-glycerate and 3-phospho-D-glyceroyl phosphate bind before ADP and ATP, respectively Saccharolobus solfataricus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.13 D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
 Saccharolobus solfataricus 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
 ?
1.2.1.13 D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
 Saccharolobus solfataricus P2 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
 ?
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Saccharolobus solfataricus ADP + 3-phospho-D-glyceroyl phosphate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Saccharolobus solfataricus P2 ADP + 3-phospho-D-glyceroyl phosphate
-
 r
4.1.2.13 Glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 Saccharolobus solfataricus D-Fructose 1,6-bisphosphate
-
 ?
4.1.2.13 Glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 Saccharolobus solfataricus P2 D-Fructose 1,6-bisphosphate
-
 ?
5.3.1.1 D-Glyceraldehyde 3-phosphate
-
 Saccharolobus solfataricus Glycerone phosphate
-
 r
5.3.1.1 D-Glyceraldehyde 3-phosphate
-
 Saccharolobus solfataricus P2 Glycerone phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
1.2.1.13 ? x * 38000, SDS-PAGE Saccharolobus solfataricus
5.3.1.1 ? x * 25000, SDS-PAGE Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
1.2.1.13 GAPDH
-
 Saccharolobus solfataricus
1.2.1.13 SSO0528 locus name Saccharolobus solfataricus
2.7.2.3 PGK
-
 Saccharolobus solfataricus
2.7.2.3 SSO0527 locus name Saccharolobus solfataricus
4.1.2.13 SSO0286 locus name Saccharolobus solfataricus
5.3.1.1 SSO2592 locus name Saccharolobus solfataricus
5.3.1.1 TpI
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.13 70
-
 assay at Saccharolobus solfataricus
2.7.2.3 70
-
 assay at Saccharolobus solfataricus
4.1.2.13 70
-
 assay at Saccharolobus solfataricus
5.3.1.1 70
-
 assay at Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.13 6.5
-
 assay at Saccharolobus solfataricus
2.7.2.3 6.5
-
 assay at Saccharolobus solfataricus
4.1.2.13 6.5
-
 assay at Saccharolobus solfataricus
5.3.1.1 6.5
-
 assay at Saccharolobus solfataricus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.2.3 1.14
-
 ADP 70°C, pH 6.5 Saccharolobus solfataricus
5.3.1.1 0.4
-
 3-phospho-D-glycerate pH 6.5, 70°C Saccharolobus solfataricus
5.3.1.1 0.661
-
 phosphoenolpyruvate pH 6.5, 70°C Saccharolobus solfataricus

General Information

EC Number General Information Comment Organism
1.2.1.13 physiological function gluconeogenic pathway Saccharolobus solfataricus
2.7.2.3 physiological function gluconeogenic pathway Saccharolobus solfataricus
4.1.2.13 physiological function gluconeogenic pathway Saccharolobus solfataricus
5.3.1.1 physiological function gluconeogenic pathway Saccharolobus solfataricus