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Literature summary extracted from

  • Chattopadhyay, A.; Foerster-Fromme, K.; Jendrossek, D.
    PQQ-dependent alcohol dehydrogenase (QEDH) of Pseudomonas aeruginosa is involved in catabolism of acyclic terpenes (2010), J. Basic Microbiol., 50, 119-124.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.5.5 gene exaA, expression of C-terminally His6-tagged enzyme in Escherichia coli strain Rosetta 2 (DE3) pLysS RARE Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.5 0.0038
-
ethanol pH 9.0, 30°C Pseudomonas aeruginosa
1.1.5.5 0.0073
-
citronellol pH 9.0, 30°C Pseudomonas aeruginosa
1.1.5.5 0.11
-
geraniol pH 9.0, 30°C Pseudomonas aeruginosa
1.1.5.5 1.3
-
citral pH 9.0, 30°C Pseudomonas aeruginosa
1.1.5.5 2.4
-
citronellal pH 9.0, 30°C Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.5 periplasm
-
Pseudomonas aeruginosa
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.5.5 Ca2+ required Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.5.5 ethanol + ubiquinone Pseudomonas aeruginosa
-
acetaldehyde + ubiquinol
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.5 Pseudomonas aeruginosa
-
gene PA1982 or exaA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.5 recombinant C-terminally His6-tagged enzyme from Escherichia coli strain Rosetta 2 (DE3) pLysS RARE by nickel affinity chromatography Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.5.5 0.1
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 1,3-butanediol Pseudomonas aeruginosa
1.1.5.5 1.3
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 1,3-propandiol Pseudomonas aeruginosa
1.1.5.5 3.1
-
purified recombinant enzyme, pH 9.0, 30°C, substrate geraniol Pseudomonas aeruginosa
1.1.5.5 3.3
-
purified recombinant enzyme, pH 9.0, 30°C, substrate citronellal Pseudomonas aeruginosa
1.1.5.5 3.8
-
purified recombinant enzyme, pH 9.0, 30°C, substrate citral Pseudomonas aeruginosa
1.1.5.5 4
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 3-methyl-1--butanol Pseudomonas aeruginosa
1.1.5.5 4.4
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 1-octanol in DMSO Pseudomonas aeruginosa
1.1.5.5 4.4
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 2-propanol Pseudomonas aeruginosa
1.1.5.5 4.5
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 1-hexanol in DMSO Pseudomonas aeruginosa
1.1.5.5 5.4
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 2-butanol Pseudomonas aeruginosa
1.1.5.5 5.6
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 1-octanol in H2O Pseudomonas aeruginosa
1.1.5.5 6.3
-
purified recombinant enzyme, pH 9.0, 30°C, substrate citronellol Pseudomonas aeruginosa
1.1.5.5 7.5
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 1-butanol Pseudomonas aeruginosa
1.1.5.5 7.7
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 1-propanol Pseudomonas aeruginosa
1.1.5.5 7.9
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 1-hexanol in H2O Pseudomonas aeruginosa
1.1.5.5 8.2
-
purified recombinant enzyme, pH 9.0, 30°C, substrate 1-pentanol Pseudomonas aeruginosa
1.1.5.5 8.5
-
purified recombinant enzyme, pH 9.0, 30°C, substrate ethanol Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.5 1,3-butandiol + ubiquinone very low activity, 0.94% of the activity with ethanol Pseudomonas aeruginosa ? + ubiquinol
-
?
1.1.5.5 1,3-propandiol + ubiquinone 15% of the activity with ethanol Pseudomonas aeruginosa ? + ubiquinol
-
?
1.1.5.5 1-butanol + ubiquinone 88% of the activity with ethanol Pseudomonas aeruginosa butanal + ubiquinol
-
?
1.1.5.5 1-hexanol + ubiquinone 93% of the activity with ethanol Pseudomonas aeruginosa hexanal + ubiquinol
-
?
1.1.5.5 1-octanol + ubiquinone 66% of the activity with ethanol Pseudomonas aeruginosa octanal + ubiquinol
-
?
1.1.5.5 1-pentanol + ubiquinone 97% of the activity with ethanol Pseudomonas aeruginosa pentanal + ubiquinol
-
?
1.1.5.5 1-propanol + ubiquinone 90% of the activity with ethanol Pseudomonas aeruginosa propanal + ubiquinol
-
?
1.1.5.5 2-butanol + ubiquinone 64% of the activity with ethanol Pseudomonas aeruginosa 2-butanone + ubiquinol
-
?
1.1.5.5 2-propanol + ubiquinone 51% of the activity with ethanol Pseudomonas aeruginosa acetone + ubiquinol
-
?
1.1.5.5 3-methyl-1-butanol + ubiquinone 48% of the activity with ethanol Pseudomonas aeruginosa 3-methyl-1-butanal + ubiquinol
-
?
1.1.5.5 citral + ubiquinol 39% of the activity with ethanol Pseudomonas aeruginosa ? + ubiquinone
-
?
1.1.5.5 citronellal + ubiquinol 45% of the activity with ethanol Pseudomonas aeruginosa citronellol + ubiquinone
-
?
1.1.5.5 citronellol + ubiquinone 74% of the activity with ethanol Pseudomonas aeruginosa citronellal + ubiquinol
-
?
1.1.5.5 ethanol + ubiquinone
-
Pseudomonas aeruginosa acetaldehyde + ubiquinol
-
?
1.1.5.5 ethanol + ubiquinone best substrate Pseudomonas aeruginosa acetaldehyde + ubiquinol
-
?
1.1.5.5 geraniol + ubiquinone 37% of the activity with ethanol Pseudomonas aeruginosa geranial + ubiquinol
-
?
1.1.5.5 additional information substrate specificity, assayed with dichlorophenolindophenol and phenazinemethosulfate as electron acceptors, overview Pseudomonas aeruginosa ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.5.5 PQQ-dependent alcohol dehydrogenase
-
Pseudomonas aeruginosa
1.1.5.5 PQQ-dependent ethanol dehydrogenase
-
Pseudomonas aeruginosa
1.1.5.5 QEDH
-
Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.5.5 30
-
assay at Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.5 9
-
assay at Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.5 pyrroloquinoline quinone dependent on, enzyme bound Pseudomonas aeruginosa

General Information

EC Number General Information Comment Organism
1.1.5.5 malfunction inactivation of PA1982 by insertion mutagenesis results in inability of the mutant to utilise ethanol and in reduced growth on geraniol. Growth on ethanol is restored by transferring an intact copy of the PA1982 gene into the mutant Pseudomonas aeruginosa
1.1.5.5 physiological function the PQQ-dependent alcohol dehydrogenase of Pseudomonas aeruginosa functions in ethanol metabolism and is involved in catabolism of acyclic terpenes, overview Pseudomonas aeruginosa