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Literature summary extracted from

  • Carpentieri, F.; de Felice, M.; de Falco, M.; Rossi, M.; Pisani, F.M.
    Physical and functional interaction between the mini-chromosome maintenance-like DNA helicase and the single-stranded DNA binding protein from the crenarchaeon Sulfolobus solfataricus (2002), J. Biol. Chem., 277, 12118-12127.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.6.2.4 single-stranded DNA binding protein stimulates activity and forms a protein/protein complex with the mini-chromosome maintenance protein helicase Saccharolobus solfataricus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.2.4 expression in Escherichia coli Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
5.6.2.4 K346A ATPase activity of the mutant is noticeably reduced, although not completely abolished, it is almost completely devoid of helicase activity Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.6.2.4 75000
-
 6 * 75000, 77428, calculated from sequence Saccharolobus solfataricus
5.6.2.4 470000
-
 gel filtration Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.4 Saccharolobus solfataricus Q9UXG1
-
-
5.6.2.4 Saccharolobus solfataricus P2 Q9UXG1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.2.4
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.2.4 ATP + H2O ATP-dependent 3'-5' DNA helicase activity in vitro. Preferentially acts on DNA duplexes containing a 5'-tail Saccharolobus solfataricus ADP + phosphate
-
 ?
5.6.2.4 ATP + H2O dATP and ATP support DNA unwinding reaction Saccharolobus solfataricus ADP + phosphate
-
 ?
5.6.2.4 ATP + H2O ATP-dependent 3'-5' DNA helicase activity in vitro. Preferentially acts on DNA duplexes containing a 5'-tail Saccharolobus solfataricus P2 ADP + phosphate
-
 ?
5.6.2.4 ATP + H2O dATP and ATP support DNA unwinding reaction Saccharolobus solfataricus P2 ADP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.6.2.4 hexamer 6 * 75000, 77428, calculated from sequence Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
5.6.2.4 MCM protein
-
 Saccharolobus solfataricus
5.6.2.4 mini-chromosome maintenance protein
-
 Saccharolobus solfataricus
5.6.2.4 Sso MCM
-
 Saccharolobus solfataricus
5.6.2.4 SSO0774 locus name Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.6.2.4 60
-
 assay at Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6.2.4 7.5
-
 assay at Saccharolobus solfataricus