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Literature summary extracted from

  • Casutt, M.S.; Huber, T.; Brunisholz, R.; Tao, M.; Fritz, G.; Steuber, J.
    Localization and function of the membrane-bound riboflavin in the Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) from Vibrio cholerae (2010), J. Biol. Chem., 285, 27088-27099.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.2.1.1 wild-type and mutant forms of the Na+-NQR from Vibrio cholerae as recombinant proteins containing a His6-tag at the N-terminus of subunit NqrA in an enzyme-deficient mutant strain DELTAnqr of Vibrio cholerae Vibrio cholerae serotype O1

Protein Variants

EC Number Protein Variants Comment Organism
7.2.1.1 additional information preparation of membranes containing subcomplexes of Na+-NQR, NqrBCDEF, NqrACDEF, NqrABDEF, NqrABCEF, and NqrABCF, by expressing mutant forms of the Na+-NQR in Vibrio cholerae enzyme-deficient mutant strain DELTAnqr Vibrio cholerae serotype O1

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.2.1.1 Ag+ specific inhibition Vibrio cholerae serotype O1

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.2.1.1 membrane
-
Vibrio cholerae serotype O1 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.2.1.1 213000
-
about, His6-tagged Na+-NQR complex, sequence calculation Vibrio cholerae serotype O1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.2.1.1 NADH + H+ + ubiquinone + n Na+/in Vibrio cholerae serotype O1
-
NAD+ + ubiquinol + n Na+/out
-
?
7.2.1.1 NADH + H+ + ubiquinone + n Na+/in Vibrio cholerae serotype O1 ATCC 39541
-
NAD+ + ubiquinol + n Na+/out
-
?

Organism

EC Number Organism UniProt Comment Textmining
7.2.1.1 Vibrio cholerae serotype O1 A5F5X1 and A5F5X0 and A5F5Y7 and A5F5Y6 and A5F5Y3 and A5F5Y4 A5F5X1: subunit NqrA, A5F5X0: subunit NqrB, A5F5Y7: subunit NqrC, A5F5Y6: subunit NqrD; A5F5Y3: subunit NqrE, A5F5Y4: subunit NqrF. The enzyme consists of six subunits encoded by the NQR operon.
-
7.2.1.1 Vibrio cholerae serotype O1 ATCC 39541 A5F5X1 and A5F5X0 and A5F5Y7 and A5F5Y6 and A5F5Y3 and A5F5Y4 A5F5X1: subunit NqrA, A5F5X0: subunit NqrB, A5F5Y7: subunit NqrC, A5F5Y6: subunit NqrD; A5F5Y3: subunit NqrE, A5F5Y4: subunit NqrF. The enzyme consists of six subunits encoded by the NQR operon.
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.2.1.1 His6-tagged wild-type and mutant forms of the Na+-NQR from Vibrio cholerae mutant strain DELTAnqr by nickel affinity chromatography and gel filtration Vibrio cholerae serotype O1

Renatured (Commentary)

EC Number Renatured (Comment) Organism
7.2.1.1 reconstitution of purified recombinant enzyme with riboflavin, enzyme is mixed with 0.1 mM riboflavin from a1 mM riboflavin stock solution in N,N-dimethylformamide, 10 min, 25°C Vibrio cholerae serotype O1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.1.1 NADH + H+ + ubiquinone + n Na+/in
-
Vibrio cholerae serotype O1 NAD+ + ubiquinol + n Na+/out
-
?
7.2.1.1 NADH + H+ + ubiquinone + n Na+/in
-
Vibrio cholerae serotype O1 ATCC 39541 NAD+ + ubiquinol + n Na+/out
-
?
7.2.1.1 NADH + H+ + ubiquinone-1 + n Na+/in ubiquinone-1 acts as substrate for the oxidation of NADH by the holo-Na+-NQR coupled to Na+ translocation. Ubiquinoone-1 also accepts electrons in a non-physiological reaction catalyzed by isolated NqrF or subcomplexes containing the NqrF subunit. Both the coupled and the non-physiological modes of NADH oxidation with Q1 require FAD as cofactor Vibrio cholerae serotype O1 NAD+ + ubiquinol-1 + n Na+/out
-
?
7.2.1.1 NADH + H+ + ubiquinone-1 + n Na+/in ubiquinone-1 acts as substrate for the oxidation of NADH by the holo-Na+-NQR coupled to Na+ translocation. Ubiquinoone-1 also accepts electrons in a non-physiological reaction catalyzed by isolated NqrF or subcomplexes containing the NqrF subunit. Both the coupled and the non-physiological modes of NADH oxidation with Q1 require FAD as cofactor Vibrio cholerae serotype O1 ATCC 39541 NAD+ + ubiquinol-1 + n Na+/out
-
?
7.2.1.1 NADH + H+ + ubiquinone-8 + n Na+/in
-
Vibrio cholerae serotype O1 NAD+ + ubiquinol-8 + n Na+/out
-
?
7.2.1.1 NADH + H+ + ubiquinone-8 + n Na+/in
-
Vibrio cholerae serotype O1 ATCC 39541 NAD+ + ubiquinol-8 + n Na+/out
-
?

Synonyms

EC Number Synonyms Comment Organism
7.2.1.1 Na+-NQR
-
Vibrio cholerae serotype O1
7.2.1.1 Na+-translocating NADH:quinone oxidoreductase
-
Vibrio cholerae serotype O1
7.2.1.1 sodium ion-translocating NADH:quinone oxidoreductase
-
Vibrio cholerae serotype O1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2.1.1 7.5
-
assay at Vibrio cholerae serotype O1

Cofactor

EC Number Cofactor Comment Organism Structure
7.2.1.1 FAD noncovalently bound FAD Vibrio cholerae serotype O1
7.2.1.1 FMN covalently bound FMN Vibrio cholerae serotype O1
7.2.1.1 NADH
-
Vibrio cholerae serotype O1
7.2.1.1 riboflavin noncovalently bound riboflavin in purified Na+-NQR is not liberated from FMN but represents an intrinsic component of the Na+-NQR complex Vibrio cholerae serotype O1

General Information

EC Number General Information Comment Organism
7.2.1.1 physiological function the sodium ion-translocating NADH:quinone oxidoreductase from the human pathogen Vibrio cholerae is a respiratory membrane protein complex that couples the oxidation of NADHto the transport of Na+ across the bacterial membrane Vibrio cholerae serotype O1