EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.3.7.7 | additional information | the ternary DPOR enzyme holocomplex comprising subunits ChlN, ChlB, and ChlL is trapped as an octameric (ChlN/ChlB)2(ChlL2)2 complex after incubation with the nonhydrolyzable ATP analogues adenosine 5'-(gamma-thio)triphosphate, adenosine 5'-(beta,gamma-imido)triphosphate, or MgADP in combination with AlF4-, complex structure, overview. A mutant ChlL2 protein, with a deleted Leu153 in the switch II region also allows for the formation of a stable octameric complex | Prochlorococcus marinus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.3.7.7 | Fe2+ | the homodimeric ChlL2 subunit carries a [4Fe-4S] cluster | Prochlorococcus marinus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.7.7 | 35000 | - |
octameric (ChlN/ChlB)2(ChlL2)2 subunit complex, 4 * 35000, subunit ChlL, + 2 * 45000, subunit ChlN, 2 * 60000, subunit ChlB, SDS-PAGE, 4 * 32395,subunit ChlL, + 2 * 46199, subunit ChlN, 2 * 58729, subunit ChlB, sequence calculation | Prochlorococcus marinus |
1.3.7.7 | 45000 | - |
octameric (ChlN/ChlB)2(ChlL2)2 subunit complex, 4 * 35000, subunit ChlL, + 2 * 45000, subunit ChlN, 2 * 60000, subunit ChlB, SDS-PAGE, 4 * 32395,subunit ChlL, + 2 * 46199, subunit ChlN, 2 * 58729, subunit ChlB, sequence calculation | Prochlorococcus marinus |
1.3.7.7 | 46199 | - |
octameric (ChlN/ChlB)2(ChlL2)2 subunit complex, 4 * 35000, subunit ChlL, + 2 * 45000, subunit ChlN, 2 * 60000, subunit ChlB, SDS-PAGE, 4 * 32395,subunit ChlL, + 2 * 46199, subunit ChlN, 2 * 58729, subunit ChlB, sequence calculation | Prochlorococcus marinus |
1.3.7.7 | 58729 | - |
octameric (ChlN/ChlB)2(ChlL2)2 subunit complex, 4 * 35000, subunit ChlL, + 2 * 45000, subunit ChlN, 2 * 60000, subunit ChlB, SDS-PAGE, 4 * 32395,subunit ChlL, + 2 * 46199, subunit ChlN, 2 * 58729, subunit ChlB, sequence calculation | Prochlorococcus marinus |
1.3.7.7 | 60000 | - |
octameric (ChlN/ChlB)2(ChlL2)2 subunit complex, 4 * 35000, subunit ChlL, + 2 * 45000, subunit ChlN, 2 * 60000, subunit ChlB, SDS-PAGE, 4 * 32395,subunit ChlL, + 2 * 46199, subunit ChlN, 2 * 58729, subunit ChlB, sequence calculation | Prochlorococcus marinus |
1.3.7.7 | 360000 | - |
enzyme complex, gel filtration | Prochlorococcus marinus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.7.7 | additional information | Prochlorococcus marinus | proposed catalytic redox cycle of DPOR, overview | ? | - |
? | |
1.3.7.7 | protochlorophyllide a + reduced ferredoxin + 2 ATP + 2 H2O | Prochlorococcus marinus | - |
chlorophyllide a + oxidized ferredoxin + 2 ADP + 2 phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.7.7 | Prochlorococcus marinus | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.7.7 | additional information | proposed catalytic redox cycle of DPOR, overview | Prochlorococcus marinus | ? | - |
? | |
1.3.7.7 | protochlorophyllide a + reduced ferredoxin + 2 ATP + 2 H2O | - |
Prochlorococcus marinus | chlorophyllide a + oxidized ferredoxin + 2 ADP + 2 phosphate | - |
? | |
1.3.7.7 | protochlorophyllide a + reduced ferredoxin + 2 ATP + 2 H2O | the homodimeric ChlL2 subunit carrying a [4Fe-4S] cluster transfers electrons to the corresponding heterotetrameric catalytic subunit (ChlN/ChlB)2, which also possesses a redox active [4Fe-4S] cluster | Prochlorococcus marinus | chlorophyllide a + oxidized ferredoxin + 2 ADP + 2 phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.7.7 | octamer | octameric (ChlN/ChlB)2(ChlL2)2 subunit complex, 4 * 35000, subunit ChlL, + 2 * 45000, subunit ChlN, 2 * 60000, subunit ChlB, SDS-PAGE, 4 * 32395,subunit ChlL, + 2 * 46199, subunit ChlN, 2 * 58729, subunit ChlB, sequence calculation | Prochlorococcus marinus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.7.7 | dark operative protochlorophyllide oxidoreductase | - |
Prochlorococcus marinus |
1.3.7.7 | DPOR | - |
Prochlorococcus marinus |
1.3.7.7 | protochlorophyllide oxidoreductase | - |
Prochlorococcus marinus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.7.7 | 25 | - |
assay at | Prochlorococcus marinus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.7.7 | ATP | - |
Prochlorococcus marinus | |
1.3.7.7 | Ferredoxin | - |
Prochlorococcus marinus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.3.7.7 | additional information | transient protein-protein interaction of ChlL2 and (ChlN/ChlB)2 is essential for the ATP-dependent electron transfer processes catalyzed by DPOR. Efficient octameric (ChlN/ChlB)2(ChlL2)2 enzyme complex formation required the presence of protochlorophyllide. Complete ATP hydrolysis is a prerequisite for intersubunit electron transfer | Prochlorococcus marinus |