EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.11.45 | construction, expression, and secretion of truncated 13R-MnLOX, from mini DNA coding for the sequence Glu-Phe-His6-Leu-Gln-Thr39-Val-Leu-Pro to Val618, in Pichia pastoris | Gaeumannomyces graminis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.13.11.45 | F337I | site-directed mutagenesis, replacement of Phe337 with Ile changes the stereochemistry of the 13-hydroperoxy metabolites of 18:2n-6 and 18:3n-3 (from 100% R to 69-74% S) with little effect on regiospecificity. The abstraction of the pro-S hydrogen of 18:2n-6 is retained, suggesting antarafacial hydrogen abstraction and oxygenation. The mutant shows highly reduced activity compared to the wild-type enzyme | Gaeumannomyces graminis |
1.13.11.45 | G332A | site-directed mutagenesis, replacement of Gly332 with the larger hydrophobic residue selectively augments dehydration of (9Z,11E,13R,15Z)-13-hydroperoxyoctadeca-9,12,15-trienoic acid and increases the oxidation at C-13 of 18:1n-6 | Gaeumannomyces graminis |
1.13.11.45 | K52N | site-directed mutagenesis | Gaeumannomyces graminis |
1.13.11.45 | L176K | site-directed mutagenesis | Gaeumannomyces graminis |
1.13.11.45 | L336A | site-directed mutagenesis, replacement of Leu336 with the smaller hydrophobic residue shifts the oxygenation from C-13 toward C-9 with formation of 9S- and 9R-hydroperoxy metabolites of 18:2n-6 and 18:3n-3, the mutant shows highly reduced activity compared to the wild-type enzyme | Gaeumannomyces graminis |
1.13.11.45 | L336F | site-directed mutagenesis, replacement of Leu336 with the larger hydrophobic residue selectively augments dehydration of (9Z,11E,13R,15Z)-13-hydroperoxyoctadeca-9,12,15-trienoic acid and increases the oxidation at C-13 of 18:1n-6, the mutant shows highly reduced activity compared to the wild-type enzyme | Gaeumannomyces graminis |
1.13.11.45 | L336G | site-directed mutagenesis, replacement of Leu336 with the smaller hydrophobic residue shifts the oxygenation from C-13 toward C-9 with formation of 9S- and 9R-hydroperoxy metabolites of 18:2n-6 and 18:3n-3, the mutant shows highly reduced activity compared to the wild-type enzyme | Gaeumannomyces graminis |
1.13.11.45 | L336V | site-directed mutagenesis, replacement of Leu336 with the smaller hydrophobic residue shifts the oxygenation from C-13 toward C-9 with formation of 9S- and 9R-hydroperoxy metabolites of 18:2n-6 and 18:3n-3, the mutant shows highly reduced activity compared to the wild-type enzyme | Gaeumannomyces graminis |
1.13.11.45 | additional information | hydrophobic replacements of Leu336 can modify the hydroperoxide configurations at C-9 with little effect on the R configuration at C-13 of the 18:2n-6 and 18:3n-3 metabolites | Gaeumannomyces graminis |
1.13.11.45 | Y158C | site-directed mutagenesis | Gaeumannomyces graminis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.45 | 0.0005 | - |
(9Z,12Z,15Z)-octadeca-9,12,15-trienoic acid | pH 9.0, temperature not specified in the publication, wild-type enzyme | Gaeumannomyces graminis | |
1.13.11.45 | 0.0027 | - |
(9Z,12Z,15Z)-octadeca-9,12,15-trienoic acid | pH 9.0, temperature not specified in the publication, mutant L336V | Gaeumannomyces graminis | |
1.13.11.45 | 0.0036 | - |
(9Z,12Z,15Z)-octadeca-9,12,15-trienoic acid | pH 9.0, temperature not specified in the publication, mutants L336A and L336F | Gaeumannomyces graminis | |
1.13.11.45 | 0.0065 | - |
(9Z,12Z,15Z)-octadeca-9,12,15-trienoic acid | pH 9.0, temperature not specified in the publication, mutant L336V | Gaeumannomyces graminis | |
1.13.11.45 | 0.0073 | - |
(9Z,12Z,15Z)-octadeca-9,12,15-trienoic acid | pH 9.0, temperature not specified in the publication, mutant F337I | Gaeumannomyces graminis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.45 | Mn2+ | manganese-containing enzyme | Gaeumannomyces graminis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.45 | (9Z,11S,12Z)-11-hydroperoxyoctadeca-9,12-dienoate + O2 | Gaeumannomyces graminis | - |
(9Z,11E, 13R)-13-hydroperoxyoctadeca-9,11-dienoate | - |
? | |
1.13.11.45 | linoleate + O2 | Gaeumannomyces graminis | - |
(9Z,11S,12Z)-11-hydroperoxyoctadeca-9,12-dienoate | - |
? | |
1.13.11.45 | additional information | Gaeumannomyces graminis | 13R-MnLOX forms (11S)-hydroperoxyoctadecadienoic acid and (11R)-hydroperoxyoctadecatrienoic acid as intermediates during the linear phase of oxidation and the (13R)-hydroperoxides as main end products | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.45 | Gaeumannomyces graminis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.45 | recombinant mini 13R-MnLOX from Pichia pastoris by hydrophobic interaction chromatography and gel filtration | Gaeumannomyces graminis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.45 | (9Z,11S,12Z)-11-hydroperoxyoctadeca-9,12-dienoate + O2 | - |
Gaeumannomyces graminis | (9Z,11E, 13R)-13-hydroperoxyoctadeca-9,11-dienoate | - |
? | |
1.13.11.45 | (9Z,11S,12Z)-11-hydroperoxyoctadeca-9,12-dienoate + O2 | - |
Gaeumannomyces graminis | (9Z,11E,13R)-13-hydroperoxyoctadeca-9,11-dienoate | - |
? | |
1.13.11.45 | (9Z,12Z,15Z)-octadeca-9,12,15-trienoic acid + O2 | - |
Gaeumannomyces graminis | ? | - |
? | |
1.13.11.45 | linoleate + O2 | - |
Gaeumannomyces graminis | (9Z,11S,12Z)-11-hydroperoxyoctadeca-9,12-dienoate | - |
? | |
1.13.11.45 | additional information | 13R-MnLOX forms (11S)-hydroperoxyoctadecadienoic acid and (11R)-hydroperoxyoctadecatrienoic acid as intermediates during the linear phase of oxidation and the (13R)-hydroperoxides as main end products | Gaeumannomyces graminis | ? | - |
? | |
1.13.11.45 | additional information | oxidation of 18:1 n-6 is performed by 13R-MnLOX | Gaeumannomyces graminis | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.11.45 | 13R-MnLOX | - |
Gaeumannomyces graminis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.13.11.45 | 9 | - |
assay at | Gaeumannomyces graminis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.13.11.45 | evolution | overview of possible lipoxygenation positions of linoleic acid and partial alignment of 13R-MnLOX, sLOX-1, 8R-LOX, human 5-LOX, and rabbit 15-LOX covering an important region for regio- and stereospecificity. A few LOXs, including 9S-MnLOX, deviate from this rule of R/S stereospecificity | Gaeumannomyces graminis |
1.13.11.45 | additional information | pentamer motif His-Val-Leu-Phe-His in MnLOX, 13R-MnLOX3 catalyzes suprafacial hydrogen abstraction and oxygenation | Gaeumannomyces graminis |