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Literature summary extracted from

  • Ozawa, Y.; Siddiqui, M.A.; Takahashi, Y.; Urushiyama, A.; Ohmori, D.; Yamakura, F.; Arisaka, F.; Imai, T.
    Indolepyruvate ferredoxin oxidoreductase: An oxygen-sensitive iron-sulfur enzyme from the hyperthermophilic archaeon Thermococcus profundus (2012), J. Biosci. Bioeng., 114, 23-27.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.8 0.015
-
 CoA pH 9.0, 80°C Thermococcus profundus
1.2.7.8 0.018
-
 phenylpyruvate in 50 mM TAPS buffer (pH 9.0), at 80°C Thermococcus profundus
1.2.7.8 0.06
-
 oxidized ferredoxin in 50 mM TAPS buffer (pH 9.0), at 80°C Thermococcus profundus
1.2.7.8 0.108
-
 phenylpyruvate pH 9.0, 80°C Thermococcus profundus
1.2.7.8 0.13
-
 4-hydroxyphenylpyruvate pH 9.0, 80°C Thermococcus profundus
1.2.7.8 0.13
-
 4-hydroxyphenylpyruvate in 50 mM TAPS buffer (pH 9.0), at 80°C Thermococcus profundus
1.2.7.8 0.22
-
 Indolepyruvate pH 9.0, 80°C Thermococcus profundus
1.2.7.8 0.22
-
 (indol-3-yl)pyruvate in 50 mM TAPS buffer (pH 9.0), at 80°C Thermococcus profundus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.8 21000
-
 2 * 67000 + 2 * 21000, SDS-PAGE Thermococcus profundus
1.2.7.8 21000
-
 2 * 67000, alpha-subunit, plus 2 * 21000, beta-subunit, SDS-PAGE Thermococcus profundus
1.2.7.8 67000
-
 2 * 67000 + 2 * 21000, SDS-PAGE Thermococcus profundus
1.2.7.8 67000
-
 2 * 67000, alpha-subunit, plus 2 * 21000, beta-subunit, SDS-PAGE Thermococcus profundus
1.2.7.8 155000
-
 sedimentation equilibrium and sedimentation velocity experiments Thermococcus profundus
1.2.7.8 170000
-
 gel filtration Thermococcus profundus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.7.8 (indol-3-yl)pyruvate + CoA + oxidized ferredoxin Thermococcus profundus
-
 S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin + H+
-
 ?
1.2.7.8 (indol-3-yl)pyruvate + CoA + oxidized ferredoxin Thermococcus profundus DSM DT5432
-
 S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.8 Thermococcus profundus
-
-
-
1.2.7.8 Thermococcus profundus DSM DT5432
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.2.7.8 25°C, aerobic conditions, half-life 7 h Thermococcus profundus
1.2.7.8 the enzyme is oxygen-sensitive with a half-life of 7 h at 25°C under aerobic conditions Thermococcus profundus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.8 purification under extremely anaerobic conditions in presence of 2mM dithiothreitol and 2mM sodium dithionite to protect against oxygen attack, and also 1mM MgSO4,1mM thiamine diphosphate and 0.2M NaCl, additives which have been found to stabilize Thermococcus profundus
1.2.7.8 under extremely anaerobic conditions with all buffers containing 2 mM dithiothreitol and 2 mM dithiothreitol. Q Sepharose column chromatography, hydroxyapatite column chromatography, Blue Sepharose column chromatography, and Superdex 200 gel filtration Thermococcus profundus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.8 1.01
-
 cell-free extract, in 50 mM TAPS buffer (pH 9.0), at 80°C Thermococcus profundus
1.2.7.8 121
-
 pH 9.0, 80°C Thermococcus profundus
1.2.7.8 121
-
 after 120fold purification, in 50 mM TAPS buffer (pH 9.0), at 80°C Thermococcus profundus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.8 (indol-3-yl)pyruvate + CoA + oxidized ferredoxin
-
 Thermococcus profundus S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin + H+
-
 ?
1.2.7.8 (indol-3-yl)pyruvate + CoA + oxidized ferredoxin
-
 Thermococcus profundus DSM DT5432 S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin + H+
-
 ?
1.2.7.8 4-hydroxyphenylpyruvate + CoA + oxidized ferredoxin
-
 Thermococcus profundus ? + CO2 + reduced ferredoxin + H+
-
 ?
1.2.7.8 4-hydroxyphenylpyruvate + CoA + oxidized ferredoxin
-
 Thermococcus profundus DSM DT5432 ? + CO2 + reduced ferredoxin + H+
-
 ?
1.2.7.8 4-hydroxyphenylpyruvate + CoA + oxidized ferredoxin
-
 Thermococcus profundus 4-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin + H+
-
 ?
1.2.7.8 indolepyruvate + CoA + oxidized methylviologen
-
 Thermococcus profundus indoleacetyl-CoA + CO2 + reduced methylviologen
-
 ?
1.2.7.8 indolepyruvate + CoA + oxidized methylviologen
-
 Thermococcus profundus DSM DT5432 indoleacetyl-CoA + CO2 + reduced methylviologen
-
 ?
1.2.7.8 phenylpyruvate + CoA + oxidized ferredoxin
-
 Thermococcus profundus phenylacetyl-CoA + CO2 + reduced ferredoxin
-
 ?
1.2.7.8 phenylpyruvate + CoA + oxidized ferredoxin
-
 Thermococcus profundus DSM DT5432 phenylacetyl-CoA + CO2 + reduced ferredoxin
-
 ?
1.2.7.8 phenylpyruvate + CoA + oxidized methyl viologen
-
 Thermococcus profundus phenylacetyl-CoA + CO2 + reduced methyl viologen + H+
-
 ?
1.2.7.8 phenylpyruvate + CoA + oxidized methyl viologen
-
 Thermococcus profundus DSM DT5432 phenylacetyl-CoA + CO2 + reduced methyl viologen + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.7.8 heterotetramer 2 * 67000 + 2 * 21000, SDS-PAGE Thermococcus profundus
1.2.7.8 tetramer 2 * 67000, alpha-subunit, plus 2 * 21000, beta-subunit, SDS-PAGE Thermococcus profundus

Synonyms

EC Number Synonyms Comment Organism
1.2.7.8 IOR
-
 Thermococcus profundus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.8 105
-
-
 Thermococcus profundus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.2.7.8 20 30 negligible activity Thermococcus profundus
1.2.7.8 60 100 dramatic increase of activity Thermococcus profundus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.7.8 60 110 a slight decrease in activity is observed below 90°C for at least 4 h. The enzyme exhibits a half-life of 62 min at 100°C, 40 min at 103°C, 20 min at 105°C, 5 min at 108°C, and less than 5 min at 110°C Thermococcus profundus
1.2.7.8 100
-
 half-life 62 min Thermococcus profundus
1.2.7.8 105
-
 half-life 20 min Thermococcus profundus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.7.8 Ferredoxin Km-value 0.06 mM Thermococcus profundus
1.2.7.8 [4Fe-4S]-center
-
 Thermococcus profundus