EC Number | Cloned (Comment) | Organism |
---|---|---|
1.4.1.2 | three genes encoding the alpha-subunit, Slgdh-NAD;A1-3, and one additional gene encoding the beta-subunit of GDH, Slgdh-NAD;B1, Slgdh-NAD;A1-3 show conserved structures, whereas Slgdh-NAD;B1 includes a 5'-untranslated exon, DNA and amino acid sequence determination and analysis, quantitative real-time PCR expresssion analysis | Solanum lycopersicum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.4.1.2 | mitochondrion | four GDH deduced amino acid sequences possess the predicted mitochondrial target peptides, 18 amino acid residues at the N-terminus | Solanum lycopersicum | 5739 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.2 | L-glutamate + H2O + NAD+ | Solanum lycopersicum | - |
2-oxoglutarate + NH3 + NADH + H+ | - |
r | |
1.4.1.2 | L-glutamate + H2O + NAD+ | Solanum lycopersicum Micro-Tom | - |
2-oxoglutarate + NH3 + NADH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.2 | Solanum lycopersicum | - |
three genes encoding the alpha-subunit, Slgdh-NAD;A1-3, and one additional gene encoding the beta-subunit of GDH, Slgdh-NAD;B1 | - |
1.4.1.2 | Solanum lycopersicum Micro-Tom | - |
three genes encoding the alpha-subunit, Slgdh-NAD;A1-3, and one additional gene encoding the beta-subunit of GDH, Slgdh-NAD;B1 | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.4.1.2 | fruit | GDH activity increases during the fruit ripening along with the content of free glutamate, the most abundant amino acid of ripe fruit involved in conferring the genuine tomato flavour. Only the active homohexamer of GDH beta-subunits is detected in roots while heterohexamers of GDH alpha- and beta-subunits are found in fruits. alpha-Subunit Slgdh-NAD;A1-3 transcripts are detected in all tomato tissues examined, showing the highest levels in mature green fruits, contrasting with beta-subunit Slgdh-NAD;B1 transcripts which are detected mainly in roots or in mature fruits when treated with glutamate, NaCl or salicylic acid | Solanum lycopersicum | - |
1.4.1.2 | leaf | - |
Solanum lycopersicum | - |
1.4.1.2 | additional information | subunit distributin and expression patterns in tissues of tomato, overview. The level of GDH alpha- and beta-subunits in tomato plants is regulated differently in each tomato organ | Solanum lycopersicum | - |
1.4.1.2 | root | only the active homohexamer of GDH beta-subunits is detected in roots while heterohexamers of GDH alpha- and beta-subunits are found in fruits. alpha-Subunit Slgdh-NAD;A1-3 transcripts are detected in all tomato tissues examined, showing the highest levels in mature green fruits, contrasting with beta-subunit Slgdh-NAD;B1 transcripts which are detected mainly in roots or in mature fruits when treated with glutamate, NaCl or salicylic acid | Solanum lycopersicum | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.2 | L-glutamate + H2O + NAD+ | - |
Solanum lycopersicum | 2-oxoglutarate + NH3 + NADH + H+ | - |
r | |
1.4.1.2 | L-glutamate + H2O + NAD+ | - |
Solanum lycopersicum Micro-Tom | 2-oxoglutarate + NH3 + NADH + H+ | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.1.2 | NADH-GDH | - |
Solanum lycopersicum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.2 | NAD+ | - |
Solanum lycopersicum | |
1.4.1.2 | NADH | - |
Solanum lycopersicum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.4.1.2 | additional information | the level of GDH alpha- and beta-subunits in tomato plants is regulated differently in each tomato organ | Solanum lycopersicum |